A5101521124- Immunotherapy and Immune Responses
- Effects of Radiation Exposure
- Plant Molecular Biology Research
- T-cell and B-cell Immunology
- Clostridium difficile and Clostridium perfringens research
- Pancreatic function and diabetes
- Immune Cell Function and Interaction
- Radiation Dose and Imaging
- Advanced Radiotherapy Techniques
- DNA Repair Mechanisms
- Plant nutrient uptake and metabolism
- Gut microbiota and health
- Genomics and Chromatin Dynamics
- Pharmacology and Obesity Treatment
- Glioma Diagnosis and Treatment
- Radiation Therapy and Dosimetry
- Diabetes and associated disorders
- Mitochondrial Function and Pathology
- Carcinogens and Genotoxicity Assessment
- Diet and metabolism studies
- Advances in Oncology and Radiotherapy
- Brain Metastases and Treatment
- CAR-T cell therapy research
- Sarcoma Diagnosis and Treatment
- RNA Research and Splicing
Louisiana State University Health Sciences Center New Orleans
2024
New York University
2013-2021
University of California, Los Angeles
2018-2020
Seattle Children's Hospital
2017-2019
University of Washington
2018-2019
University of California, Irvine
2018
Columbia University
2017
Memorial Sloan Kettering Cancer Center
2015-2017
Duke University Hospital
2001-2016
Duke Medical Center
2001-2016
A prerequisite for strong adaptive antiviral immunity is the robust initial activation of innate immune system, which frequently mediated by TLR-activated plasmacytoid DCs (pDCs). Natural antitumor often comparatively weak, potentially due to lack TLR-mediated signals within tumor microenvironment. To assess whether pDCs are capable directly facilitating effective responses, mice bearing established subcutaneous B16 melanoma tumors were administered TLR9-activated into tumor. We found that...
Significance Our study exploits time—the relatively unexplored fourth dimension of gene regulatory networks (GRNs)—to learn the temporal transcriptional logic underlying dynamic nitrogen (N) signaling in plants. We introduce several conceptual innovations to analysis time-series data area predictive GRNs. resulting network now provides “transcriptional logic” for transcription factor perturbations aimed at improving N-use efficiency, an important issue global food production marginal soils...
Cardiomyopathy frequently complicates sepsis and is associated with increased mortality. Increased cardiac oxidative stress mitochondrial dysfunction have been observed during sepsis, but the mechanisms responsible for these abnormalities not determined. We hypothesized that NADPH oxidase 2 (NOX2) activation could be sepsis-induced cardiomyopathy. Treatment of isolated adult mouse cardiomyocytes low concentrations endotoxin lipopolysaccharide (LPS) total cellular reactive oxygen species...
Antibiotic resistance among enterococci and γ-proteobacteria is an increasing problem in healthcare settings. Dense colonization of the gut by antibiotic-resistant bacteria facilitates their spread between patients also leads to bloodstream other systemic infections. Antibiotic-mediated destruction intestinal microbiota consequent loss are critical factors leading persistence bacteria. The mechanisms underlying microbiota-mediated remain incompletely defined likely distinct for different...
Multivariate analysis reveals a hormone- and nutrient-regulated network that shapes root architecture.
Inferring phenotypic outcomes from genomic features is both a promise and challenge for systems biology. Using gene expression data to predict outcomes, functionally validating the genes with predictive powers are two challenges we address in this study. We applied an evolutionarily informed machine learning approach phenotypes based on transcriptome responses shared within across species. Specifically, exploited diversity nitrogen use efficiency conserved treatments Arabidopsis accessions...
Abstract Plasmacytoid dendritic cells (pDC) are capable of producing high levels type I IFNs upon viral stimulation, and play a central role in modulating innate adaptive immunity against infections. Whereas many studies have assessed myeloid (mDC) the induction antitumor immune responses, pDC has not been addressed. Moreover, interaction with other cell subsets evaluated. In this study, we analyzed capacity stimulating an Ag-specific T response. Immunization mice Ag-pulsed, activated...
Dendritic cells (DCs) have been well characterized for their ability to initiate cell-mediated immune responses by stimulating naive T cells. However, the use of DCs stimulate antigen-activated in vivo has not investigated. In this study, we determined whether DC vaccination could improve efficacy activated, adoptively transferred induce an enhanced antitumor response. Mice bearing B16 melanoma tumors expressing gp100 tumor antigen were treated with cultured, activated transgenic a T-cell...
Significance Species display a range of plastic phenotypes that presumably have evolved as result adaptation to heterogeneous environments. We asked whether the genetic mechanisms underlie across populations also determine response an individual plant environmental cues in Arabidopsis . Using integrative root phenotyping approach, genes natural variation architecture were shown control plasticity responses within individual. Together, our results uncover mechanism underlying phenotypic and...
Clostridium difficile infection (CDI) is a frequent complication in recipients of allogeneic hematopoietic stem cell transplantation (allo-HSCT), who receive intensive treatments that significantly disrupt the intestinal microbiota. In this study, we examined microbiota composition allo-HSCT to identify bacterial colonizers confer protection against CDI after engraftment.Feces collected from adult at engraftment were analyzed; 16S ribosomal RNA genes sequenced and analyzed each sample....
Long-term immunity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) requires the identification of T-cell epitopes affecting host immunogenicity. In this computational study, we explored CD8 + epitope diversity estimated in 27 most common HLA-A and HLA-B alleles, representing United States population. Analysis 16 SARS-CoV-2 variants [B.1, Alpha (B.1.1.7), five Delta (AY.100, AY.25, AY.3, AY.3.1, AY.44), nine Omicron (BA.1, BA.1.1, BA.2, BA.4, BA.5, BQ.1, BQ.1.1, XBB.1,...
The Medtronic MiniMed 670G system with SmartGuard™ (Medtronic, Northridge, CA) is a commercial hybrid closed-loop (HCL) approved for use in 2018 children >7 years. Studies of this HCL subjects years old show improvement glycemic control, but no study has described its younger children. This retrospective analysis patients type 1 diabetes (T1D) <7 age who used the at Seattle Children's Hospital 3 months. We compared 2-week data from Carelink™ while manual mode (MM) suspend before low active...
Background: Pulmonary tuberculosis (PTB) is associated with many forms of chronic lung disease including the development airflow obstruction (AFO). However, nature, evolution and mechanisms responsible for AFO after PTB are poorly understood. The aim this study was to examine progression changes in physiology patients treated PTB. Methods: Immunocompetent, previously healthy, adult receiving ambulatory treatment a first episode were prospectively followed up serial quantitative computed...
Abstract Purpose: The identification of tumor antigens recognized by cytotoxic and T helper lymphocytes has led to the development specific cancer vaccines. Immunization with antigen-pulsed dendritic cells proved effective at eliciting elevated levels antigen–specific in patient blood, but objective clinical responses remain rare, suggesting that vaccine-induced are not trafficking optimally site(s) burden. Accumulating evidence from animal models suggests route immunization can have a...
Objectives/Goals: The primary objective of this study is to investigate the relationship between human leukocyte antigen (HLA) alleles COVID-19 clinical severity, specifically: hospitalization, mortality, pneumonia by COVID-19, post-acute sequelae SARS-CoV-2 infection (PASC), and lab values. Methods/Study Population: We are conducting a retrospective cohort utilizing All Us controlled tier dataset. base population was defined as any patients with diagnosis code (ICD-10: U07.1 or SNOMED:...
Recurrent viral diseases often occur after the viruses evade hosts' immune system, by inducing exhaustion of antiviral T cells. In present study, we found that functionally exhausted herpes simplex virus type 1 (HSV-1) -specific CD8+ cells, with elevated expression lymphocyte activation gene-3 (LAG-3), an checkpoint receptor promotes cell exhaustion, were frequent in symptomatic (SYMP) patients a history numerous episodes recurrent corneal herpetic disease. Similarly, following UV-B induced...
To examine the effectiveness and safety of single-isocenter multitarget stereotactic radiosurgery using a volume-adapted dosing strategy in patients with 4 to 10 brain metastases.Adult metastases were eligible for this prospective trial. The primary endpoint was overall survival. Secondary endpoints local recurrence, distant failure, neurologic death, rate adverse events. Exploratory objectives neurocognition, quality life, dosimetric data, salvage rate, radionecrosis. Dose prescribed single...
The objective of this retrospective study was to determine the potential benefits chemotherapy in esophageal cancer patients treated with chemoradiation followed by surgery.At our institution, 145 completed trimodality therapy from 1993 2009. Neoadjuvant treatment predominantly consisted 5-fluorouracil and cisplatin a concurrent median radiation dose 50.4 Gy. Sixty-two received postoperatively. majority (49/62) 3 cycles docetaxel.Within entire cohort, 5-year overall survival (OS) benefit...
The relationship between added sugar and arterial stiffness in youth with type 1 diabetes (T1D) has not been well-described. We used data from the SEARCH for Diabetes Youth Study (SEARCH), an ongoing observational cohort study, to determine association individuals diagnosed T1D <20 years of age (n = 1539; mean duration 7.9 ± 1.9 years). Added intake was assessed by a food frequency questionnaire, measures included pulse wave velocity (PWV) augmentation index. Separate multivariate linear...
Mutations in LMNA encoding lamin A/C and EMD emerin cause cardiomyopathy muscular dystrophy. Lmna null mice develop these disorders have a lifespan of 7-8 weeks. Emd show no overt pathology normal skeletal muscle but with regeneration defects. We generated germline deletions both to determine the effects combined loss encoded proteins. Mice without are born at expected Mendelian ratio, grossly birth shorter lifespans than those lacking only A/C. However, there major differences between...