A5008907987- Plant Molecular Biology Research
- Plant Reproductive Biology
- Chromosomal and Genetic Variations
- Plant Gene Expression Analysis
- Light effects on plants
- Genetic Mapping and Diversity in Plants and Animals
- Plant nutrient uptake and metabolism
- Plant Virus Research Studies
- Plant tissue culture and regeneration
- Genomics and Phylogenetic Studies
- Single-cell and spatial transcriptomics
- Plant Stress Responses and Tolerance
- Plant Disease Resistance and Genetics
- Plant and Biological Electrophysiology Studies
- Photosynthetic Processes and Mechanisms
- Plant and Fungal Interactions Research
- Plant Genetic and Mutation Studies
- RNA Research and Splicing
- Cell Image Analysis Techniques
- Molecular Biology Techniques and Applications
- Lipid metabolism and biosynthesis
- Leaf Properties and Growth Measurement
- Plant responses to water stress
- Genetics and Plant Breeding
- GABA and Rice Research
University of Tübingen
2016-2025
Cold Spring Harbor Laboratory
2012-2023
Institute of Plant Molecular Biology
2020
Truman State University
2012
Iowa State University
2004-2012
Cornell University
2004-2012
University of Georgia
2012
École Normale Supérieure de Lyon
2011
Université Claude Bernard Lyon 1
2011
Centre National de la Recherche Scientifique
2011
Abstract Background Long non-coding RNAs (lncRNAs) are transcripts that 200 bp or longer, do not encode proteins, and potentially play important roles in eukaryotic gene regulation. However, the number, characteristics expression inheritance pattern of lncRNAs maize still largely unknown. Results By exploiting available public EST databases, whole genome sequence annotation RNA-seq datasets from 30 different experiments, we identified 20,163 putative lncRNAs. Of these lncRNAs, more than 90%...
MicroRNAs and trans -acting siRNAs (ta-siRNAs) have important regulatory roles in development. Unlike other developmentally molecules, small RNAs are not known to act as mobile signals during Here, we show that low-abundant, conserved ta-siRNAs, termed tasiR-ARFs, move intercellularly from their defined source of biogenesis on the upper (adaxial) side leaves lower (abaxial) create a gradient patterns abaxial determinant AUXIN RESPONSE FACTOR3. Our observations ramifications for function...
Abstract KNOTTED1-like homeobox (KNOX) genes promote stem cell activity and must be repressed to form determinate lateral organs. Stable KNOX gene silencing during organogenesis is known involve the predicted DNA binding proteins ASYMMETRIC LEAVES1 (AS1) AS2 as well chromatin-remodeling factor HIRA. However, mechanism of unknown. Here, we show that AS1 a repressor complex binds directly regulatory motifs CWGTTD KMKTTGAHW present at two sites in promoters BREVIPEDICELLUS (BP) KNAT2. The act...
The leaf vasculature plays a key role in solute translocation. Veins consist of at least seven distinct cell types, with specific roles transport, metabolism, and signaling. Little is known about vascular cells, particular the phloem parenchyma (PP). PP effluxes sucrose into apoplasm as basis for loading, yet has been characterized only microscopically. Here, we enriched cells from Arabidopsis leaves to generate single-cell transcriptome atlas vasculature. We identified 19 clusters,...
Small RNAs are important regulators of gene expression. In maize, adaxial/abaxial (dorsoventral) leaf polarity is established by an abaxial gradient microRNA166 (miR166), which spatially restricts the expression domain class III homeodomain leucine zipper (HD-ZIPIII) transcription factors that specify adaxial/upper fate. Here, we show leafbladeless1 encodes a key component in trans -acting small interfering RNA (ta-siRNA) biogenesis pathway acts on adaxial side developing leaves and...
Dorsoventral (adaxial/abaxial) polarity of the maize leaf is established in meristem and maintained throughout organ development to coordinate proper outgrowth patterning leaf. rolled leaf1 (rld1) leafbladeless1 (lbl1) are required for specification adaxial/upper surface. rld1 encodes a class III homeodomain-leucine zipper (HD-ZIPIII) protein whose adaxial expression spatially defined by miRNA166-directed transcript cleavage on abaxial side. The semi-dominant Rld1-Original (Rld1-O) mutation,...
Abstract Plant shoots are characterized by indeterminate growth resulting from the action of a population stem cells in shoot apical meristem (SAM). Indeterminacy within SAM is specified part class I knox homeobox genes. The myb domain proteins rough sheath2 (RS2) and ASYMMETRIC LEAVES1 (AS1) maize (Zea mays) Arabidopsis thaliana, respectively, required to establish determinacy during leaf development. These cellular memory system that response cell–derived signal keeps genes an off state...
Polycomb-repressive complexes (PRCs) ensure the correct spatiotemporal expression of numerous key developmental regulators. Despite their pivotal role, how PRCs are recruited to specific targets remains largely unsolved, particularly in plants. Here we show that Arabidopsis ASYMMETRIC LEAVES complex physically interacts with PRC2 and recruits this homeobox genes BREVIPEDICELLUS KNAT2 stably silence these stem cell regulators differentiating leaves. The recruitment mechanism resembles...
MicroRNAs (miRNAs) and trans-acting siRNAs (ta-siRNAs) are essential to the establishment of adaxial–abaxial (dorsoventral) leaf polarity. Tas3-derived ta-siRNAs define adaxial side by restricting expression domain miRNA miR166, which in turn demarcates abaxial leaves determinants. To investigate regulatory mechanisms that allow for precise spatiotemporal accumulation these polarizing small RNAs, we used laser-microdissection coupled RT-PCR determine profiles their precursor transcripts...
The complex genomes of many economically important crops present tremendous challenges to understand the genetic control quantitative traits with great importance in crop production, adaptation, and evolution. Advances genomic technology need be integrated strategic design novel perspectives break new ground. Complementary individual-gene–targeted research, which remains challenging, a global assessment distribution trait-associated SNPs (TASs) discovered from genome scans can provide...
Abstract The maize shoot apical meristem (SAM) comprises a small pool of stem cells that generate all above-ground organs. Although mutational studies have identified genetic networks regulating SAM function, little is known about morphological variation in natural populations. Here we report the use high-throughput image processing to capture rich size within diverse inbred panel. We demonstrate correlations between seedling and agronomically important adult traits such as flowering time,...
Abstract Mobile small RNAs serve as local positional signals in development and coordinate stress responses across the plant. Despite its central importance, an understanding of how cell-to-cell movement is governed lacking. Here, we show that miRNA mobility precisely regulated through a gating mechanism polarised at defined cell–cell interfaces. This generates directional between neighbouring cells limits long-distance shoot-to-root trafficking, underpins domain-autonomous behaviours within...
ABSTRACT The maize leafbladeless1 (lbl1) mutant displays a variety of leaf and plant phenotypes. most extreme manifestation in the is formation radially symmetric, abaxialized leaves due to complete loss adaxial cell types. Less severe phenotypes, resulting from partial identity, include ectopic laminae at boundary between abaxialized, sectors on surface bifurcation leaves. Ectopic bifurcations arise early development result an altered patterning along proximodistal axis, or duplication...
All above-ground plant organs are derived from shoot apical meristems (SAMs). Global analyses of gene expression were conducted on maize (Zea mays L.) SAMs to identify genes preferentially expressed in the SAM. The collected 14-day-old B73 seedlings via laser capture microdissection (LCM). RNA samples extracted LCM-collected and hybridized microarrays spotted with 37 660 cDNAs. Approximately 30% (10 816) these cDNAs prepared as part this study manually dissected apices. Over 5000 sequence...
Abstract Transcription factors of the plant-specific homeodomain leucine zipper IV (HD-ZIP IV) family have been found from moss to higher plants, and several members associated with epidermis-related expression and/or function. In maize (Zea mays), four five characterized HD-ZIP are expressed specifically in epidermis, one contributes trichome development, target genes another involved cuticle biosynthesis. Assessing phylogeny, synteny, gene structure, expression, regulation entire maize, 12...
Plant small RNAs are 3' methylated by the methyltransferase HUA1 ENHANCER1 (HEN1). In plant hen1 mutants, modifications of RNAs, including oligo-uridylation (tailing), associated with accelerated degradation microRNAs (miRNAs). By sequencing wild type and mutants from Arabidopsis thaliana, rice (Oryza sativa), maize (Zea mays), we found truncation prior to tailing is widespread in these mutants. Moreover, patterns miRNA differ substantially among families but conserved across species. The...