A5037424740- Plant Molecular Biology Research
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Polyamine Metabolism and Applications
- CRISPR and Genetic Engineering
- Plant tissue culture and regeneration
- Plant Stress Responses and Tolerance
- Polysaccharides and Plant Cell Walls
- Leaf Properties and Growth Measurement
- Light effects on plants
- Advanced biosensing and bioanalysis techniques
- Plant Gene Expression Analysis
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Legume Nitrogen Fixing Symbiosis
- Plant Virus Research Studies
- Algal biology and biofuel production
- Chromosomal and Genetic Variations
- Insect symbiosis and bacterial influences
- RNA regulation and disease
- Cellular transport and secretion
- Plant nutrient uptake and metabolism
- Machine Learning in Bioinformatics
- Plant Parasitism and Resistance
- Bacteriophages and microbial interactions
Instituto de Biología Molecular y Celular de Plantas
2011-2025
Universitat de València
2011-2025
Consejo Superior de Investigaciones Científicas
2002-2022
Universitat Politècnica de València
2008-2022
Hospital de Manises
2020
Laboratoire Physiologie Cellulaire & Végétale
2010-2015
Institut National de la Recherche Agronomique
2015
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2010-2011
Université Joseph Fourier
2010-2011
Centre National de la Recherche Scientifique
2010-2011
Plant development is modulated by the convergence of multiple environmental and endogenous signals, mechanisms that allow integration different signaling pathways currently being unveiled. A paradigmatic case concurrence brassinosteroid (BR) gibberellin (GA) in control cell expansion during photomorphogenesis, which supported physiological observations several plants but for no molecular mechanism has been proposed. In this work, we show these two occurs through physical interaction between...
Polyamine biosynthesis is an ancient metabolic pathway present in all organisms. Aminopropyltransferases are key enzymes that mediate the synthesis of spermidine, spermine, and thermospermine. The relatively high sequence similarity between aminopropyltransferases their with putrescine N-methyltransferases (PMT) raises question whether they share a common ancestor or have evolved by convergence. Here we show PMT phylogenetically interconnected, different activities been generated unusually...
Cell size and secondary cell wall patterning are crucial for the proper functioning of xylem vessel elements in vascular tissues plants. Through detailed anatomical characterization Arabidopsis thaliana hypocotyls, we observed that mutations putative spermine biosynthetic gene ACL5 severely affected specification: acl5 mutant were small mainly spiral type, normally predominant pitted vessels as well fibers completely missing. The cell-specific expression early developing elements, detected...
Abstract Despite great advances in sequencing technologies, generating functional information for nonmodel organisms remains a challenge. One solution lies an improved ability to predict genetic circuits based on primary DNA sequence combination with detailed knowledge of regulatory proteins that have been characterized model species. Here, we focus the LEAFY (LFY) transcription factor, conserved master regulator floral development. Starting biochemical and structural information, built...
Plasticity and robustness of signaling pathways partly rely on genetic redundancy, although the precise mechanism that provides functional specificity to different redundant elements in a given process is often unknown. In Arabidopsis, redundancy gibberellin has been largely attributed presence five members DELLA family transcriptional regulators. Here, we demonstrate two evolutionarily functionally divergent proteins, RGL2 RGA, can perform exchangeable functions when they are expressed...
The CRISPR/Cas12a editing system opens new possibilities for plant genome engineering. To obtain a comparative assessment of RNA-guided endonuclease (RGEN) types in plants, we adapted the to GoldenBraid (GB) modular cloning platform and compared efficiency Acidaminococcus (As) Lachnospiraceae (Lb) Cas12a variants with previously described GB-assembled Streptococcus pyogenes Cas9 (SpCas9) constructs eight Nicotiana benthamiana loci using transient expression. All three nucleases showed...
Significance DELLA proteins are plant-specific transcriptional regulators that act as signaling hubs at the interface between environment and networks control growth. The growth-promoting hormone gibberellin destabilizes DELLAs. Here we describe an alternative pathway to destabilize these proteins. We show DELLAs substrate of COP1, E3 ubiquitin ligase increases its activity promote growth in response shade or warmth. Our results not changes levels, mediates rapid destabilization environmental cues.
The conversion of putrescine to spermidine in the biosynthetic pathway plant polyamines is catalyzed by two closely related synthases, SPDS1 and SPDS2, Arabidopsis. In yeast two-hybrid system, SPDS2 was found interact with a novel protein, SPMS (spermine synthase), which homologous SPDS1. interacts both vitro. Unlike failed suppress speΔ3 deficiency synthase yeast. However, able complement speΔ4 spermine yeast, indicating that synthase. SPDS proteins showed no homodimerization but formed...
The apical hook develops in the upper part of hypocotyl when seeds buried soil germinate, and serves to protect cotyledons shoot meristem from possible damage caused by pushing through soil. curvature is formed differential cell growth that occurs at two opposite sides hypocotyl, it established a gradient auxin activity refined coordinated action ethylene. Here we show gibberellins (GAs) promote development transcriptional regulation several genes ethylene pathways Arabidopsis. level GA...
The role of the tetraamine spermine in plant defense against pathogens was investigated by using Arabidopsis (Arabidopsis thaliana)-Pseudomonas viridiflava pathosystem. effects perturbations levels on susceptibility to bacterial infection were evaluated transgenic plants (35S::spermine synthase [SPMS]) that overexpressed SPMS gene and accumulated spermine, as well spms mutants with low levels. former exhibited higher resistance P. than wild-type plants, while latter more susceptible....
Efficient elimination of the editing machinery remains a challenge in plant biotechnology after genome to minimize probability off-target mutations, but it is also important deliver end users with edited plants free foreign DNA. Using modular cloning system Golden Braid we have included fluorescence-dependent transgene monitoring module tool box. We tested this approach Solanum lycopersicum, Oryza sativa and Arabidopsis thaliana. demonstrate that DsRED fluorescence visualization works...
The demand for saffron is expected to rise in the coming years due its nutraceutical and medicinal properties. To cope with this, it will be necessary develop a mechanised production of saffron. Upgrading methods requires accurate control flowering time this species. Nevertheless, little known about Crocus sativus L. aim study gain insight into floral induction regulatory networks operating A transcriptomic analysis was performed from main buds different stages development. identification...
SUMMARY Legumes have unique features, such as compound inflorescences and a complex floral ontogeny. Thus, the study of regulatory genes in these species during inflorescence development is essential to understand their role evolutionary origin developmental novelties. The SUPERMAN ( SUP ) gene encodes C2H2 zinc‐finger transcriptional repressor that regulates organ number third fourth whorls Arabidopsis thaliana . In this work, we present functional characterization Medicago truncatula MtSUP...
p53 AND DNA DAMAGE-REGULATED GENE1 (PDRG1) is part of the prefoldin-like complex (PFDLc) in plants and animals. Whether PDRG1 acts primarily as a subunit PFDLc or an independent not known any eukaryote. Here, we show that impairment activity Arabidopsis thaliana leads to embryonic lethality, case for other proteins UXT AtURI. The subunits are main interactors vivo, interactomes PDRG1, UXT, AtURI strong overlaps, including nuclear RNA polymerases various complexes spliceosome. Our results...
Transcriptional networks are central to any biological process and changes affecting transcription factors or their binding sites in the genome a key factor driving evolution. As more organisms being sequenced, tools needed easily predict (TFBS) presence affinity from mere inspection of genomic sequences. Although many TFBS discovery algorithms exist, for using DNA models they generate relatively scarce use is limited among biologist community by lack flexible user-friendly tools. We have...
High temperature is a general stress factor that causes decrease in crop yield. It has been shown auxin application reduces the male sterility caused by exposure to higher temperatures. However, widespread of hormone with vast effects on plant physiology may be discouraged many cases. Therefore, generation new varieties locally enhance reproductive organs represent an alternative strategy. We have explored possibility increasing indole-3-acetic acid (IAA) ovaries reducing IAA...
De-etiolation is the first developmental process under light control allowing heterotrophic seedling to become autotrophic. The phytohormones cytokinins (CKs) largely contribute this process. Reversible phosphorylation a key event of cell signaling, proteins active or generating binding site for specific protein interaction. 14-3-3 regulate variety plant responses. expression, hormonal regulation, and proteomic network 14-3-3s were addressed in tomato (Solanum lycopersicum L.) during blue...
The development of high nitrogen use efficiency (NUE) cultivars under low N inputs is required for sustainable agriculture. To this end, in study, we analyzed the impact long-term suboptimal conditions on metabolome and transcriptome tomato to identify specific molecular processes regulators at organ scale. Physiological metabolic analysis revealed responses maintain glutamate, asparagine, sucrose synthesis leaves partition sustain growth, while assimilated C surplus stored roots....
Guide RNA design for CRISPR genome editing of gene families is a challenging task as usually good candidate sgRNAs are tagged with low scores precisely because they match several locations in the genome, thus time-consuming manual evaluation targets required. To address this issues, I have developed ARES-GT, Python local command line tool compatible any operative system. ARES-GT allows selection that multiple input query sequences, addition specifically each sequence. It also contemplates...
Coat protein I (COPI) is necessary for intra-Golgi transport and retrograde from the Golgi apparatus back to endoplasmic reticulum. The key component of COPI coat coatomer complex, which composed seven subunits (α/β/β’/γ/δ/ε/ζ) recruited en bloc cytosol onto membranes. In mammals yeast, α- β’-COP WD40 domains mediate cargo-selective interactions with dilysine motifs present in canonical cargoes vesicles. contrast three isoforms (β’1-3-COP) have been identified Arabidopsis. To understand role...