A5017034081- Light effects on plants
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Plant biochemistry and biosynthesis
- Antioxidant Activity and Oxidative Stress
- Plant Gene Expression Analysis
- Photoreceptor and optogenetics research
- Plant Reproductive Biology
- Ubiquitin and proteasome pathways
- Polyamine Metabolism and Applications
- Greenhouse Technology and Climate Control
- Artificial Immune Systems Applications
- Heat shock proteins research
- Agricultural Practices and Plant Genetics
- Receptor Mechanisms and Signaling
- Gene Regulatory Network Analysis
- Rice Cultivation and Yield Improvement
- Irrigation Practices and Water Management
- Plant responses to water stress
- Lipid metabolism and biosynthesis
- Cancer, Hypoxia, and Metabolism
Wageningen University & Research
2025
James Hutton Institute
2024
Lancaster University
2018-2022
University of Edinburgh
2014-2018
Institute of Structural and Molecular Biology
2014
Center for Research in Agricultural Genomics
2010-2013
Kyoto University
2007-2012
Universidad Nacional Autónoma de México
2012
Institut de Biologia Molecular de Barcelona
2010
Agricultural Research Service
2003-2006
Abstract The basic/helix-loop-helix (bHLH) proteins are a superfamily of transcription factors that bind as dimers to specific DNA target sites and have been well characterized in nonplant eukaryotes important regulatory components diverse biological processes. Based on evidence the bHLH protein PIF3 is direct phytochrome reaction partner photoreceptor's signaling network, we undertaken comprehensive computational analysis Arabidopsis genome sequence databases define scope features family....
The ability to interpret daily and seasonal alterations in light temperature signals is essential for plant survival. This particularly important during seedling establishment when the phytochrome photoreceptors activate photosynthetic pigment production photoautotrophic growth. Phytochromes accomplish this partly through suppression of interacting factors (PIFs), negative regulators chlorophyll carotenoid biosynthesis. While bZIP transcription factor long hypocotyl 5 (HY5), a potent PIF...
Carotenoids are key for plants to optimize carbon fixing using the energy of sunlight. They contribute light harvesting but also channel away from chlorophylls protect photosynthetic apparatus excess light. Phytochrome-mediated signals major cues regulating carotenoid biosynthesis in plants, we still lack fundamental knowledge on components this signaling pathway. Here show that phytochrome-interacting factor 1 (PIF1) and other transcription factors (PIF) family down-regulate accumulation...
Carotenoids are photosynthetic pigments essential for the protection against excess light. During deetiolation, their production is regulated by a dynamic repression-activation module formed PHYTOCHROME-INTERACTING FACTOR1 (PIF1) and LONG HYPOCOTYL5 (HY5). These transcription factors directly oppositely control expression of gene encoding PHYTOENE SYNTHASE (PSY), first main rate-determining enzyme carotenoid pathway. Antagonistic modules also regulate responses deetiolated plants to...
Abstract In previous time-resolved microarray-based expression profiling, we identified 32 genes encoding putative transcription factors, signaling components, and unknown proteins that are rapidly robustly induced by phytochrome (phy)-mediated light signals. Postulating they the most likely to be direct targets of phy function in primary regulatory circuitry, examined impact targeted mutations these on phy-induced seedling deetiolation process Arabidopsis thaliana. Using light-imposed...
Plastids provide plants with metabolic pathways that are unique among eukaryotes, including the methylerythritol 4-phosphate pathway for production of isoprenoids essential photosynthesis and plant growth. Here, we show first enzyme pathway, deoxyxylulose 5-phosphate synthase (DXS), interacts J-protein J20 in Arabidopsis thaliana. J-proteins typically act as adaptors substrate specificity to heat shock protein 70 (Hsp70), a molecular chaperone. Immunoprecipitation experiments showed DXS...
Stomata govern the gaseous exchange between leaf and external atmosphere, their function is essential for photosynthesis global carbon oxygen cycles. Rhythmic stomata movements in daily dark/light cycles prevent water loss at night allow CO2 uptake during day. How actors involved are transcriptionally regulated how this might contribute to rhythmicity largely unknown. Here, we show that morning opening depends on previous period. The transcription factors PHYTOCHROME-INTERACTING FACTORS...
The Arabidopsis MADS box transcription factor APETALA1 (AP1) was identified as a substrate for farnesyltransferase and shown to be farnesylated efficiently both in vitro vivo. AP1 regulates the transition from inflorescence shoot floral meristems development of sepals petals. fused green fluorescent protein (GFP) retained activity directed expected terminal flower phenotype when ectopically expressed transgenic Arabidopsis. However, ap1mS, farnesyl cysteine–acceptor mutant AP1, well...
In plants, light receptors play a pivotal role in photoperiod sensing, enabling them to track seasonal progression. Photoperiod sensing arises from an interaction between the plant's endogenous circadian oscillator and external cues. Here, we characterize of phytochrome A (phyA) sensing. Our metaanalysis functional genomic datasets identified phyA as principal regulator morning-activated genes, specifically short photoperiods. We demonstrate that PHYA expression is under direct control...
Summary The plastidial methylerythritol phosphate ( MEP ) pathway is an essential route for plants as the source of precursors all isoprenoids, many which are medical and biotechnological importance. highly sensitive to environmental cues these compounds linked photosynthesis growth light one main regulatory factors. However, mechanisms coordinating with not fully understood. Here we demonstrate that by a differential direct transcriptional modulation, via key‐master integrators signal...
Abstract The red phytochrome and blue cryptochrome plant photoreceptors play essential roles in promoting genome-wide changes nuclear chloroplastic gene expression for photomorphogenesis, plastid development, greening. While their importance anterograde signalling has been long recognized, the molecular mechanisms involved remain under active investigation. More recently, intertwining of light cascades with retrograde signals optimization chloroplast functions acknowledged. Advances field...
Prenylation is necessary for association of the petunia calmodulin CaM53 with plasma membrane. To determine whether post-prenylation processing protein was also required membrane targeting, we studied subcellular localization a GFP-labelled reporter in yeast and plant cells. Blocking carboxyl-methylation prenylated proteins either by specific inhibitor or mutant cells resulted green fluorescence to what appears be endomembrane system, contrast observed control We show that prenyl-cysteine...
To promote photomorphogenesis, including plastid development and metabolism, the phytochrome (phy) cryptochrome (cry) photoreceptors orchestrate genome‐wide changes in gene expression response to Red (R)‐ Blue (B)‐light cues. While phys crys have a clear role modulating photosynthesis, their coordination of nuclear genome plastome, essential for functional chloroplasts, remains underexplored. Using publicly available datasets WT phyABCDE or cry1cry2 Arabidopsis seedlings, grown,...
Phytochrome regulates various physiological and developmental processes throughout the life cycle of plants. Among members phytochrome family, A (phyA) exclusively mediates far-red light high irradiance response (FR-HIR), which is elicited by continuous light. In FR-HIR, nuclear accumulation phyA, precedes responses, proposed to be required for response. contrast FR, red induces rapid degradation phyA suppress undesirable long-term photomorphogenic responses phyA. present study, we compared...
Phytochrome is a red (R)/far-red (FR) light–sensing photoreceptor that regulates various aspects of plant development. Among the members phytochrome family, A (phyA) exclusively mediates atypical responses, such as FR high irradiance response (FR-HIR), which elicited under prolonged FR. proteasome-based degradation pathway rapidly eliminates active Pfr (the FR-absorbing form phyA) R. To elucidate structural basis for phyA-specific properties, we systematically constructed 16 chimeric...
Summary Prenylation is necessary for association of the petunia calmodulin CaM53 with plasma membrane. To determine whether post‐prenylation processing protein was also required membrane targeting, we studied subcellular localization a GFP‐labelled reporter in yeast and plant cells. Blocking carboxyl‐methylation prenylated proteins either by specific inhibitor or mutant cells resulted green fluorescence to what appears be endomembrane system, contrast observed control We show that...
Societal Impact Statement Tomatoes are important to agriculture, human nutrition and cuisines globally. However, many commercial tomato varieties, including the saladette that dominates North American market, highly sensitive environmental changes impact yields critical biochemical pathways carotenoids isoprenoids influence nutritional content flavour. We highlight potential of agrodiversity, notably its genetic diversity, as an undervalued research tool for understanding regulation plant...