A5053500698- Plant Molecular Biology Research
- Leaf Properties and Growth Measurement
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Remote Sensing in Agriculture
- Plant Stress Responses and Tolerance
- Spectroscopy and Chemometric Analyses
- Photosynthetic Processes and Mechanisms
- Light effects on plants
- Smart Agriculture and AI
- Greenhouse Technology and Climate Control
- Genomics and Chromatin Dynamics
- Plant responses to water stress
- Image Processing Techniques and Applications
- Gene expression and cancer classification
- Plant tissue culture and regeneration
- Optical Coherence Tomography Applications
- Genetics and Plant Breeding
- Chromosomal and Genetic Variations
- Plant Gene Expression Analysis
- Plant Water Relations and Carbon Dynamics
- Microtubule and mitosis dynamics
- Cell Image Analysis Techniques
- Tree Root and Stability Studies
- Plant pathogens and resistance mechanisms
Ghent University
2015-2024
Vlaams Instituut voor Biotechnologie
2009-2024
VIB-UGent Center for Plant Systems Biology
2014-2023
Research International (United States)
2012
Ghent University Hospital
2012
University of Antwerp
2010-2011
Max Planck Institute for Developmental Biology
2010
Max Planck Institute of Molecular Plant Physiology
2010
University of Nottingham
2010
Centrum Wiskunde & Informatica
2010
Auxin transport, which is mediated by specialized influx and efflux carriers, plays a major role in many aspects of plant growth development. AUXIN1 (AUX1) has been demonstrated to encode high-affinity auxin carrier. In Arabidopsis thaliana, AUX1 belongs small multigene family comprising four highly conserved genes (i.e., LIKE [LAX] LAX1, LAX2, LAX3). We report that all members this AUX/LAX display uptake functions. Despite the conservation their biochemical function, AUX1, LAX3 have...
Abstract TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR1 (TCP) transcription factors control developmental processes in plants. The 24 TCP encoded the Arabidopsis (Arabidopsis thaliana) genome are divided into two classes, class I and II TCPs, which proposed to act antagonistically. We performed a detailed phenotypic analysis of tcp20 mutant, showing an increase leaf pavement cell sizes 10-d-old seedlings. Subsequently, glucocorticoid receptor induction assay was performed, aiming...
The final size of plant organs, such as leaves, is tightly controlled by environmental and genetic factors that must spatially temporally coordinate cell expansion cycle activity. However, this regulation organ growth still poorly understood. aim study to gain more insight into the control leaf in Arabidopsis (Arabidopsis thaliana) performing a comparative analysis transgenic lines produce enlarged leaves under standardized conditions. To end, we selected five genes belonging different...
Abstract Leaf growth is a complex developmental process that continuously fine-tuned by the environment. Various abiotic stresses, including mild drought stress, have been shown to inhibit leaf in Arabidopsis (Arabidopsis thaliana), but underlying mechanisms remain largely unknown. Here, we identify redundant transcription factors ETHYLENE RESPONSE FACTOR5 (ERF5) and ERF6 as master regulators adapt environmental changes. ERF5 gene expression induced very rapidly specifically actively growing...
Abstract The transcriptional coactivator ANGUSTIFOLIA3 (AN3) stimulates cell proliferation during Arabidopsis thaliana leaf development, but the molecular mechanism is largely unknown. Here, we show that inducible nuclear localization of AN3 initial growth results in differential expression important regulators, including GROWTH REGULATING FACTORs (GRFs). Chromatin purification further revealed presence at loci GRF5, GRF6, CYTOKININ RESPONSE FACTOR2, CONSTANS-LIKE5 (COL5), HECATE1 (HEC1),...
Abstract Although the response of plants exposed to severe drought stress has been studied extensively, little is known about how adapt their growth under mild conditions. Here, we analyzed leaf and rosette six Arabidopsis (Arabidopsis thaliana) accessions originating from different geographic regions when stress. The automated phenotyping platform WIWAM was used impose early during development, third emerges shoot apical meristem. Analysis growth-related phenotypes showed differences in...
Summary Brassinosteroid ( BR ) hormones control plant growth through acting on both cell expansion and division. Here, we examined the role of s in leaf using A rabidopsis ‐deficient mutant constitutive photomorphogenesis dwarfism cpd ). We show that reduced size blades is a result decrease number, as well venation length complexity. Kinematic analysis tissue‐specific marker gene expression revealed phenotype associated with prolonged division phase delayed differentiation. ‐leaf‐rescue...
In multicellular organisms, morphogenesis relies on a strict coordination in time and space of cell proliferation differentiation. contrast to animals, plant development displays continuous organ formation adaptive growth responses during their lifespan relying tight proliferation. How developmental signals interact with the cell-cycle machinery is largely unknown. Here, we characterize A2-type cyclins, small gene family mitotic show how they contribute fine-tuning local development....
The phytohormone cytokinin has been shown to affect many aspects of plant development ranging from the regulation shoot apical meristem leaf senescence. However, some studies have reported contradictory effects on physiology. Therefore treatments cause both chlorosis and increased greening lead decrease or increase in cell size. To elucidate this multifaceted role development, we employed a system temporal controls over pool investigated consequences modulated levels third Arabidopsis. We...
Plant growth and crop yield are negatively affected by a reduction in water availability. However, clear understanding of how is regulated under nonlethal drought conditions lacking. Recent advances genomics, phenomics, transcriptomics allow in-depth analysis natural variation. In this study, we conducted detailed screening leaf responses to mild worldwide collection Arabidopsis thaliana accessions. The genetic architecture the upon was investigated subjecting different phenotypes...
SHORT-ROOT (SHR) and SCARECROW (SCR) are required for stem cell maintenance in the Arabidopsis (Arabidopsis thaliana) root meristem, ensuring its indeterminate growth. Mutation of SHR SCR genes results disorganization quiescent center loss activity, resulting cessation This paper reports on role development leaves, which, contrast to root, have a determinate growth pattern lack persistent niche. Our demonstrate that inhibition leaf shr scr mutants is not secondary effect compromised but...
The anaphase-promoting complex/cyclosome (APC/C) is a large multiprotein E3 ubiquitin ligase involved in ubiquitin-dependent proteolysis of key cell cycle regulatory proteins, including the destruction mitotic cyclins at metaphase-to-anaphase transition. Despite its importance, role APC/C plant cells and regulation activity during division remain poorly understood. Here, we describe identification plant-specific negative regulator complex, designated SAMBA. In Arabidopsis thaliana, SAMBA...
Most living organisms developed systems to efficiently time environmental changes. The plant-clock acts in coordination with external signals generate output responses determining seasonal growth and flowering time. Here, we show that two Arabidopsis thaliana transcription factors, FAR1 RELATED SEQUENCE 7 (FRS7) FRS12, act as negative regulators of these processes. These proteins accumulate particularly short-day conditions interact form a complex. Loss-of-function FRS7 FRS12 results early...
Abstract To efficiently capture sunlight for photosynthesis, leaves typically develop into a flat and thin structure. This development is driven by cell division expansion, but the individual contribution of these processes currently unknown, mainly because experimental difficulties to disentangle them in developing organ, due their tight interconnection. circumvent this problem, we built mathematic model that describes possible patterns expansion rates epidermal cells. was used fit data on...
The study of transgenic Arabidopsis lines with altered vascular patterns has revealed key players in the venation process, but details vascularization process are still unclear, partly because most have only been assessed qualitatively. Therefore, quantitative analyses required to identify subtle perturbations pattern and test dynamic modeling hypotheses using biological measurements. We developed an online framework, designated Leaf Image Analysis Interface (LIMANI), which automatically...
Endoreduplication is the process where a cell replicates its genome without mitosis and cytokinesis, often followed by differentiation. This alternative cycle results in various levels of endoploidy, reaching 4× or higher one haploid set chromosomes. found animals widespread plants, it plays major role cellular differentiation plant development. Here, we show that variation endoreduplication between Arabidopsis thaliana accessions Columbia-0 Kashmir controlled two quantitative trait loci,...