A5089463162- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Light effects on plants
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Plant Stress Responses and Tolerance
- Plant Parasitism and Resistance
- Leaf Properties and Growth Measurement
- RNA modifications and cancer
- RNA Research and Splicing
- Plant Gene Expression Analysis
- Polymer-Based Agricultural Enhancements
- Antioxidant Activity and Oxidative Stress
- Plant Physiology and Cultivation Studies
- Greenhouse Technology and Climate Control
- Plant-Microbe Interactions and Immunity
- Circadian rhythm and melatonin
- 14-3-3 protein interactions
- Plant biochemistry and biosynthesis
- Plant Micronutrient Interactions and Effects
- Genetic Mapping and Diversity in Plants and Animals
- RNA and protein synthesis mechanisms
- Insect-Plant Interactions and Control
Bielefeld University
2008-2018
Wageningen University & Research
2011-2014
Centre for BioSystems Genomics
2013
University of Dundee
2012
James Hutton Institute
2012
Research International (United States)
2012
MRC Laboratory of Molecular Biology
2011
Abstract TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR1 (TCP) transcription factors control developmental processes in plants. The 24 TCP encoded the Arabidopsis (Arabidopsis thaliana) genome are divided into two classes, class I and II TCPs, which proposed to act antagonistically. We performed a detailed phenotypic analysis of tcp20 mutant, showing an increase leaf pavement cell sizes 10-d-old seedlings. Subsequently, glucocorticoid receptor induction assay was performed, aiming...
Analyses of the functions TEOSINTE-LIKE1, CYCLOIDEA, and PROLIFERATING CELL FACTOR1 (TCP) transcription factors have been hampered by functional redundancy between its individual members. In general, putative functionally redundant genes are predicted based on sequence similarity confirmed genetic analysis. TCP family, however, identification is impeded relatively low overall similarity. a search for pairs that control Arabidopsis leaf development, this work performed an integrative...
The RNA binding protein AtGRP7 is part of a circadian slave oscillator in Arabidopsis thaliana that negatively autoregulates its own mRNA, and affects the levels other transcripts. Here, we identify novel role for as flowering-time gene. An atgrp7-1 T-DNA mutant flowers later than wild-type plants under both long short days, independent interference lines with reduced AtGRP7, closely related AtGRP8 protein, are also late flowering, particularly photoperiods. Consistent retention...
Alternative splicing (AS) of pre-mRNAs is an important regulatory mechanism shaping the transcriptome. In plants, only few RNA-binding proteins are known to affect AS. Here, we show that glycine-rich protein AtGRP7 influences AS in Arabidopsis thaliana. Using a high-resolution RT–PCR-based panel, found significant changes ratios isoforms for 59 288 analyzed events upon ectopic expression. particular, affected choice alternative 5′ splice sites preferentially. About half also influenced by...
Plants show a suite of inducible defense responses against bacterial pathogens. Here we investigate in detail the effect circadian clock on these reactions Arabidopsis thaliana. The magnitude immune elicited by flg22, virulent and avirulent Pseudomonas syringae strains depends time day inoculation. oxidative burst is stronger when flg22 infiltrated morning wild-type plants but not arrhythmic mutant lux arrhythmo/phytoclock1 (pcl1), thus controlled endogenous clock. Similarly, bacteria are...
Alternative splicing (AS) gives rise to multiple mRNA isoforms from the same gene, providing possibilities regulate gene expression beyond level of transcription. In a recent paper in Nucleic Acids Research we used high resolution RT-PCR based panel study changes AS patterns plants with altered levels an hnRNP-like RNA-binding protein Arabidopsis thaliana. Furthermore, detected significant between different ecotypes. Here investigated how small ambient temperature affect AS. We found for 12...
The analysis of circadian leaf movement rhythms is a simple yet effective method to study effects treatments or gene mutations on the clock plants. Currently, movements are analysed using time lapse photography and subsequent bioinformatics analyses movements. Programs that used for this purpose either able perform one function (i.e. tip detection rhythm analysis) their limited specific computational environments. We developed tool—PALMA—that works in command line combines image extraction...
Understanding the effect of cis-regulatory elements (CRE) and clusters CREs, which are called modules (CRM), in eukaryotic gene expression is a challenge computational biology. We developed two programs that allow simple, fast reliable analysis candidate CREs CRMs may affect specific determine positional features between individual within CRM. The first program, "Exploration Distinctive CRMs" (EDCC), correlates with patterns. For pairs EDCC also determines preferences single relation to each...