A5042094783- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Light effects on plants
- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Plant Reproductive Biology
- Circadian rhythm and melatonin
- Plant Stress Responses and Tolerance
- Plant Virus Research Studies
- Plant tissue culture and regeneration
- Plant-Microbe Interactions and Immunity
- Plant nutrient uptake and metabolism
- Genomics and Phylogenetic Studies
- Plant and Biological Electrophysiology Studies
- Cancer-related gene regulation
- Plant Gene Expression Analysis
- Plant Pathogenic Bacteria Studies
- Plant responses to water stress
- Plant Physiology and Cultivation Studies
- Plant pathogens and resistance mechanisms
- Molecular Biology Techniques and Applications
- Nutrition, Genetics, and Disease
- Toxin Mechanisms and Immunotoxins
- Plant Micronutrient Interactions and Effects
Bielefeld University
2015-2024
Center for Systems Biology Dresden
2013
James Hutton Institute
2012
University of Dundee
2012
University of British Columbia
2008
Hertfordshire Community NHS Trust
2008
École Polytechnique Fédérale de Lausanne
1993-2003
University of Freiburg
2003
ETH Zurich
1997-2003
Swiss Finance Institute
2002
The endogenous clock that drives circadian rhythms is thought to communicate temporal information within the cell via cycling downstream transcripts. A transcript encoding a glycine-rich RNA-binding protein, Atgrp7 , in Arabidopsis thaliana undergoes oscillations with peak levels evening. At GRP7 protein also cycles time delay so decline when accumulates high levels. After concentration has fallen trough levels, starts reaccumulate. Overexpression of transgenic plants severely depresses...
Summary The clock‐regulated RNA‐binding protein AtGRP7 is part of a negative feedback circuit through which the influences circadian oscillations its own transcript. Constitutive overexpression in transgenic plants leads to appearance low amount an alternatively spliced Atgrp7 transcript with premature stop codon. It generated by use 5′ cryptic splice site middle intron at expense fully mRNA, indicating role for selection. Accelerated decay this accounts steady state abundance. This...
The Arabidopsis RNA-binding protein AtGRP8 undergoes negative autoregulation at the post-transcriptional level. An elevated level promotes use of a cryptic 5' splice site to generate an alternatively spliced transcript, as_AtGRP8, retaining half intron with premature termination codon. In mutants defective in nonsense-mediated decay (NMD) abundance as_AtGRP8 but not its pre-mRNA is elevated, indicating that direct NMD target, thus limiting production functional protein. addition own...
In the chalcone synthase gene of Antirrhinum majus (snapdragon), 150 base pairs 5' flanking region contain cis-acting signals for UV light-induced expression. A nuclear factor, designated CG-1, specifically recognizes a hexameric motif with internal dyad symmetry, CACGTG, located within this light-responsive sequence. Binding CG-1 is influenced by C-methylation CpG dinucleotide in recognition factor found variety dicotyledonous plant species including Nicotiana tabacum, A. majus, Petunia...
The RNA binding protein AtGRP7 is part of a circadian slave oscillator in Arabidopsis thaliana that negatively autoregulates its own mRNA, and affects the levels other transcripts. Here, we identify novel role for as flowering-time gene. An atgrp7-1 T-DNA mutant flowers later than wild-type plants under both long short days, independent interference lines with reduced AtGRP7, closely related AtGRP8 protein, are also late flowering, particularly photoperiods. Consistent retention...
Alternative splicing (AS) of pre-mRNAs is an important regulatory mechanism shaping the transcriptome. In plants, only few RNA-binding proteins are known to affect AS. Here, we show that glycine-rich protein AtGRP7 influences AS in Arabidopsis thaliana. Using a high-resolution RT–PCR-based panel, found significant changes ratios isoforms for 59 288 analyzed events upon ectopic expression. particular, affected choice alternative 5′ splice sites preferentially. About half also influenced by...
The environmentally responsive signaling pathways that link global transcriptomic changes through alternative splicing (AS) to plant fitness remain unclear. Here, we found the interaction of extracellular rapid alkalinization FACTOR 1 (RALF1) peptide with its receptor FERONIA (FER) triggered a and massive RNA AS response by interacting phosphorylating glycine-rich binding protein7 (GRP7) elevate GRP7 nuclear accumulation in Arabidopsis thaliana. FER-dependent phosphorylation enhanced mRNA...
Functions for RNA-binding proteins in orchestrating plant development and environmental responses are well established. However, the lack of a genome-wide view their vivo binding targets landscapes represents gap understanding mode action proteins. Here, we adapt individual nucleotide resolution crosslinking immunoprecipitation (iCLIP) to determine repertoire circadian clock-regulated Arabidopsis thaliana glycine-rich protein AtGRP7.iCLIP identifies 858 transcripts with significantly...
Abstract N6-methyladenosine (m6A) emerges as an important modification in eukaryotic mRNAs. m6A has first been reported 1974, and its functional significance mammalian gene regulation importance for proper development have well established. An arsenal of writer, eraser, reader proteins accomplish deposition, removal, interpretation the mark, resulting dynamic function. This led to concept epitranscriptome, compendium RNA species with chemical nucleobases cell, analogy epigenome. While long...
Accurate and comprehensive annotation of transcript sequences is essential for quantification differential gene expression analysis. Single-molecule long-read sequencing technologies provide improved integrity structures including alternative splicing, transcription start polyadenylation sites. However, accuracy significantly affected by errors, mRNA degradation, or incomplete cDNA synthesis.We present a new Arabidopsis thaliana Reference Transcript Dataset 3 (AtRTD3). AtRTD3 contains over...
Specific recognition of N6 -methyladenosine (m 6 A) in mRNA by RNA-binding proteins containing a YT521-B homology (YTH) domain is important eukaryotic gene regulation. The Arabidopsis YTH protein ECT2 thought to bind at URU(m A)Y sites, yet RR(m A)CH the canonical m A consensus site all eukaryotes and functions require A-binding activity. Here, we apply iCLIP ( i ndividual nucleotide resolution c ross l inking mmuno p recipitation) HyperTRIBE t argets R NA-binding dentified b y e diting)...
Summary To investigate physiological processes generated by endogenous circadian rhythms on the molecular level, we have identified clock‐controlled genes In long‐day plant Sinapis aiba L. A cDNA library was differentially screened using probes representing transcripts expressed at either middle of light period or dark period. Two closely related groups transcripts, Sa grp1 and grp2, controlled a rhythm been isolated. RNA blot analysis verified that transcript levels oscillate in plants...
Summary The clock‐regulated RNA‐binding protein At GRP7 ( Arabidopsis thaliana glycine‐rich protein) influences circadian oscillations of its transcript by negative feedback at the post‐transcriptional level. Here we show that site‐specific mutation one conserved arginine to glutamine within RNA recognition motif impairs binding recombinant pre‐mRNA in vitro . This correlates with loss auto‐regulation vivo : transgenic plants constitutively overexpressing GRP7‐ox), a shift occurs an...