A5058834831- Plant Molecular Biology Research
- RNA Research and Splicing
- RNA modifications and cancer
- Plant Reproductive Biology
- Plant Virus Research Studies
- Photosynthetic Processes and Mechanisms
- RNA and protein synthesis mechanisms
- Microtubule and mitosis dynamics
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Peanut Plant Research Studies
- Plant and Fungal Interactions Research
- Agricultural pest management studies
- Plant-Microbe Interactions and Immunity
- Plant Disease Resistance and Genetics
- Plant tissue culture and regeneration
- Plant Gene Expression Analysis
- Genomics and Phylogenetic Studies
- Plant Disease Management Techniques
- Plant Genetic and Mutation Studies
- Legume Nitrogen Fixing Symbiosis
- Chromosomal and Genetic Variations
- Light effects on plants
- Biochemical and Structural Characterization
- Crystallization and Solubility Studies
Colorado State University
2016-2025
Hebrew University of Jerusalem
2023
Chettinad Health City
2022
University of Florida
2019
Institut des Sciences des Plantes de Montpellier
2012
Max Perutz Labs
2011
Medical University of Vienna
2011
International Crops Research Institute for the Semi-Arid Tropics
1991-2010
Universität Hamburg
2006
University of Hyderabad
1994-2006
In recent years the kinesin superfamily has become so large that several different naming schemes have emerged, leading to confusion and miscommunication. Here, we set forth a standardized nomenclature based on 14 family designations. The scheme unifies all previous phylogenies proposals, while allowing individual sequence names remain same, for expansion occur as new sequences are discovered.
Abstract Alternative splicing and alternative polyadenylation (APA) of pre-mRNAs greatly contribute to transcriptome diversity, coding capacity a genome gene regulatory mechanisms in eukaryotes. Second-generation sequencing technologies have been extensively used analyse transcriptomes. However, major limitation short-read data is that it difficult accurately predict full-length splice isoforms. Here we sequenced the sorghum using Pacific Biosciences single-molecule real-time long-read...
Summary Precursor mRNAs with introns can undergo alternative splicing (AS) to produce structurally and functionally different proteins from the same gene. Here, we show that pre‐mRNAs of Arabidopsis genes encode serine/arginine‐rich (SR) proteins, a conserved family regulators in eukaryotes, are extensively alternatively spliced. Remarkably about 95 transcripts produced only 15 genes, thereby increasing complexity SR gene transcriptome by six‐fold. The AS some is controlled developmental...
Abstract There are no comprehensive methods to identify N 6 -methyladenosine (m A) at single-base resolution for every single transcript, which is necessary the estimation of m A abundance. We develop a new pipeline called Nanom6A identification and quantification modification using Nanopore direct RNA sequencing based on an XGBoost model. validate our method methylated immunoprecipitation (MeRIP-Seq) A-sensitive RNA-endoribonuclease–facilitated (m6A-REF-seq), confirming high accuracy. Using...
In plants, calcium (Ca2+) has emerged as an important messenger mediating the action of many hormonal and environmental signals, including biotic abiotic stresses. Many different signals raise cytosolic concentration ([Ca2+]cyt), which in turn is thought to regulate cellular developmental processes via Ca2+-binding proteins. Three out four classes proteins plants contain EF-hand motif(s). This motif a conserved helix-loop-helix structure that can bind single Ca2+ ion. To identify all...
Analysis of the recently completed Arabidopsis genome sequence indicates that approximately 31% predicted genes could not be assigned to functional categories, as they do show any similarity with proteins known function from other organisms. Calmodulin (CaM), a ubiquitous and multifunctional Ca(2+) sensor, interacts wide variety cellular modulates their activity/function in regulating diverse processes. However, primary amino acid CaM-binding domain different (CBPs) is conserved. One way...
Kinesins constitute a superfamily of microtubule motor proteins that are found in eukaryotic organisms. Members the kinesin perform many diverse cellular functions such as transport vesicles and organelles, spindle formation elongation, chromosome segregation, dynamics morphogenesis. Only few kinesins have been characterized plants including Arabidopsis thaliana. Because which involved, number, types characteristics present genome would provide valuable information for researchers.Here we...
Growing interest in alternative splicing plants and the extensive sequencing of new plant genomes necessitate more precise definition classification genes coding for factors. SR proteins are a family RNA binding proteins, which function as essential factors constitutive splicing. We propose unified nomenclature taking into account newly revised mammalian number plant-specific properties proteins. identify six subfamilies Arabidopsis thaliana rice (Oryza sativa), three specific. The proposed...
We propose a method for predicting splice graphs that enhances curated gene models using evidence from RNA-Seq and EST alignments. Results obtained experiments in Arabidopsis thaliana show predictions made by our SpliceGrapher are more consistent with current than TAU Cufflinks. Furthermore, analysis of plant human data indicates the machine learning approach used is useful discriminating between real spurious sites, can improve reliability detection alternative splicing. available download...
Abiotic and biotic stresses limit crop productivity. Exposure to a non-lethal stress, referred as priming, can allow plants survive subsequent otherwise lethal conditions; the priming effect persists even after prolonged stress-free period. However, molecular mechanisms underlying are not fully understood. Here, we investigated basis of heat-shock memory role in Arabidopsis thaliana. Comprehensive analysis transcriptome-wide changes gene expression alternative splicing primed non-primed...
Recently we reported that CRLK1, a novel calcium/calmodulin-regulated receptor-like kinase plays an important role in regulating plant cold tolerance. Calcium/calmodulin binds to CRLK1 and upregulates its activity. Gene knockout complementation studies revealed is positive regulator of response chilling freezing temperatures. Here show MEKK1, member MAP family, interacts with both vitro planta. The triggered activation wild-type plants was abolished crlk1 mutants. Similarly, the induced...
Drought is a major limiting factor of crop yields. In response to drought, plants reprogram their gene expression, which ultimately regulates multitude biochemical and physiological processes. The timing this reprogramming the nature drought-regulated genes in different genotypes are thought confer differential tolerance drought stress. Sorghum highly drought-tolerant has been increasingly used as model cereal identify that tolerance. Also, there considerable natural variation resistance...