A5088027601- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- Ubiquitin and proteasome pathways
- Photosynthetic Processes and Mechanisms
- Protist diversity and phylogeny
- Cellular Mechanics and Interactions
- Genetics, Bioinformatics, and Biomedical Research
- Cellular transport and secretion
- Nuclear Structure and Function
- Chromosomal and Genetic Variations
- Mitochondrial Function and Pathology
- Micro and Nano Robotics
- Plant nutrient uptake and metabolism
- Cancer-related Molecular Pathways
- Genomic variations and chromosomal abnormalities
- Plant Molecular Biology Research
- Epigenetics and DNA Methylation
- 14-3-3 protein interactions
- Cardiomyopathy and Myosin Studies
- Connective tissue disorders research
- Reproductive Biology and Fertility
- Advanced Fluorescence Microscopy Techniques
- Polyamine Metabolism and Applications
- Cell Adhesion Molecules Research
- Hippo pathway signaling and YAP/TAZ
Indiana University School of Medicine
2019-2025
Indiana University Bloomington
2015-2025
Indiana University
2010-2021
Indiana University – Purdue University Indianapolis
2019
Indiana University Health
2019
Max Planck Society
2011
University of Pittsburgh
2007
Division of Undergraduate Education
2006
University of California, San Francisco
1996-1999
University of California, Davis
1999
In recent years the kinesin superfamily has become so large that several different naming schemes have emerged, leading to confusion and miscommunication. Here, we set forth a standardized nomenclature based on 14 family designations. The scheme unifies all previous phylogenies proposals, while allowing individual sequence names remain same, for expansion occur as new sequences are discovered.
Previous genetic and biochemical studies have led to the hypothesis that essential mitotic bipolar kinesin, KLP61F, cross-links slides microtubules (MTs) during spindle assembly function. Here, we tested this by immunofluorescence immunoelectron microscopy (immunoEM). We show Drosophila embryonic spindles at metaphase anaphase contain abundant bundles of MTs running between poles. These interpolar MT are parallel near poles antiparallel in midzone. observed KLP61F motors, phosphorylated a...
The complex behavior of chromosomes during mitosis is accomplished by precise binding and highly regulated polymerization dynamics kinetochore microtubules. Previous studies have implicated Kin Is, unique kinesins that depolymerize microtubules, in regulating chromosome positioning. We characterized the immunofluorescence localization centromere-bound MCAK found localized to inner kinetochores prophase but was predominantly centromeric metaphase. Interestingly, accumulated at leading...
Kinesin-14 family proteins are minus-end directed motors that cross-link microtubules and play key roles during spindle assembly. We showed previously the Xenopus XCTK2 is regulated by Ran via association of a bipartite NLS in tail with importin alpha/beta, which regulates its ability to formation. Here we show mutation nuclear localization signal (NLS) human HSET caused an accumulation cytoplasm, resulted strong microtubule bundling. overexpression HeLa cells longer spindles, similar what...
The dynamic activities of cellular microtubules (MTs) are tightly regulated by a balance between MT-stabilizing and -destabilizing proteins. Studies in Xenopus egg extracts have shown that the major MT destabilizer during interphase mitosis is kinesin-related protein XKCM1, which depolymerizes ends an ATP-dependent manner. Herein, we examine effects both overexpression inhibition XKCM1 on regulation dynamics vertebrate somatic cells. We found MT-destabilizing enzyme PtK2 cells modulates...
We used a peptide antibody to conserved sequence in the motor domain of kinesins screen Xenopus ovary cDNA expression library. Among clones isolated were two that encoded protein we named XCTK2 for COOH-terminal kinesin 2. contains an NH2-terminal globular domain, central α-helical stalk, and domain. is similar CTKs other organisms most homologous CHO2. Antibodies raised against recognize 75-kD egg extracts cosediments with microtubules. In tissue culture cells, anti-XCTK2 antibodies stain...
The small GTPase Ran is essential for spindle assembly. proposed to act through its nuclear import receptors importin alpha and/or beta control the sequestration of proteins necessary To date, molecular mechanisms by which pathway functions remain unclear. Using purified proteins, we have reconstituted Ran-regulated microtubule binding C-terminal kinesin XCTK2, a important We show that tail XCTK2 binds microtubules and this inhibited in presence (alpha/beta) restored addition Ran-GTP....
During mitosis, mitotic centromere-associated kinesin (MCAK) localizes to chromatin/kinetochores, a cytoplasmic pool, and spindle poles. Its localization activity in the chromatin region are regulated by Aurora B kinase; however, how cytoplasmic- pole-localized MCAK is currently not clear. In this study, we used Xenopus egg extracts form spindles absence of centrosomes found that tightly A. This regulation important focus microtubules at aster centers facilitate transition from asters...
Chromosome congression and segregation require the proper attachment of microtubules to two sister kinetochores. Disruption either Aurora B kinase or Kinesin-13 mitotic centromere-associated kinesin (MCAK) increases chromosome misalignment missegregation due improper kinetochore–microtubule attachments. MCAK localization activity are regulated by B, but how phosphorylation affects spindle assembly is unclear. Here, we show that binding arms also B-dependent arm centromere distinct two-site...