A5081016920- Nuclear Structure and Function
- Microtubule and mitosis dynamics
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Ubiquitin and proteasome pathways
- DNA Repair Mechanisms
- Pluripotent Stem Cells Research
- Cellular Mechanics and Interactions
- RNA Interference and Gene Delivery
- Coral and Marine Ecosystems Studies
- Cellular transport and secretion
- 14-3-3 protein interactions
- Skin and Cellular Biology Research
- Epigenetics and DNA Methylation
- Molecular Biology Techniques and Applications
- Plant Molecular Biology Research
- Aquaculture disease management and microbiota
- Genetics, Bioinformatics, and Biomedical Research
- Cancer Cells and Metastasis
- Virus-based gene therapy research
- Immune Cell Function and Interaction
- Developmental Biology and Gene Regulation
- IL-33, ST2, and ILC Pathways
- Cancer-related Molecular Pathways
- Angiogenesis and VEGF in Cancer
Zhongkai University of Agriculture and Engineering
2025
Carnegie Institution for Science
2015-2024
Department of Embryology
2013-2023
Johns Hopkins University
2001-2022
Zhangzhou Normal University
2020
China Telecom (China)
2018
China Telecom
2018
National Cancer Institute
2016
Soonchunhyang University
2015
University of California, Berkeley
2015
Pericentrin and γ-tubulin are integral centrosome proteins that play a role in microtubule nucleation organization. In this study, we examined the relationship between these cytoplasm at centrosome. extracts prepared from Xenopus eggs, were part of large complex as demonstrated by sucrose gradient sedimentation, gel filtration coimmunoprecipitation analysis. The pericentrin–γ-tubulin was distinct previously described ring (γ-TuRC) purified γ-TuRC fractions did not contain detectable...
The nuclear lamina is a key structural element of the metazoan nucleus. However, organization major proteins composing poorly defined. Using three-dimensional structured illumination microscopy and computational image analysis, we characterized supramolecular structures lamin A, C, B1, B2 in mouse embryo fibroblast nuclei. Each isoform forms distinct fiber meshwork, with comparable physical characteristics respect to mesh edge length, face area shape, connectivity form faces. Some...
The guanosine triphosphatase Ran stimulates assembly of microtubule asters and spindles in mitotic Xenopus egg extracts. A carboxyl-terminal region the nuclear-mitotic apparatus protein (NuMA), a nuclear required for organizing spindle poles, mimics Ran's ability to induce asters. This NuMA fragment also specifically interacted with transport factor, importin-β. We show that importin-β is an inhibitor aster extracts regulates interaction between NuMA. Importin-β therefore links regulation by...
γ-tubulin exists in two related complexes Drosophila embryo extracts (Moritz, M., Y. Zheng, B.M. Alberts, and K. Oegema. 1998. J. Cell Biol. 142:1– 12). Here, we report the purification characterization of both that name small complex (γTuSC; ∼280,000 D) γTuRC (∼2,200,000 D). In addition to γ-tubulin, γTuSC contains Dgrip84 Dgrip91, proteins homologous Spc97/98p protein family. The is a structural subunit γTuRC, larger containing about six additional polypeptides. Like isolated from Xenopus...
Mitotic spindle morphogenesis is a series of highly coordinated movements that lead to chromosome segregation and cytokinesis. We report the intermediate filament protein lamin B, component interphase nuclear lamina, functions in assembly. Lamin B assembled into matrix-like network mitosis through process depended on presence guanosine triphosphate-bound form small triphosphatase Ran. Depletion resulted defects Dominant negative mutant proteins disrupt assembly nuclei also disrupted mitosis....
Proper chromosome segregation requires the attachment of sister kinetochores to microtubules from opposite spindle poles form bi-oriented chromosomes on metaphase spindle. The passenger complex containing Survivin and kinase Aurora B regulates this process centromeres. We report that a de-ubiquitinating enzyme, hFAM, alignment by controlling both dynamic association with centromeres proper targeting is ubiquitinated in mitosis through Lys(48) Lys(63) ubiquitin linkages. de-ubiquitination...
Tremendous progress has been made in understanding the functions of γ-tubulin and, particular, its role microtubule nucleation since publication discovery 1989. The structure determined, and components complexes have identified. Significant ring complex led to a persuasive model for how these nucleate assembly. At same time, data accumulated that important but less well understood are not simply consequence function nucleation. These include roles regulation plus-end dynamics, gene...
Many corals harbour symbiotic dinoflagellate algae. The algae live inside coral cells in a specialized membrane compartment known as the symbiosome, which shares photosynthetically fixed carbon with host while provide inorganic to for photosynthesis
Significance Interactions between the cell nucleus and cytoskeleton regulate mechanics are facilitated by interplay nuclear lamina linker of nucleoskeleton (LINC) complexes. To date, specific contribution four lamin isoforms to nucleocytoskeletal connectivity whole-cell remains unknown. We discover that A- B-type lamins distinctively interact with LINC complexes bind F-actin vimentin filaments differentially modulate cortical stiffness, cytoplasmic contractility mouse embryonic fibroblasts...
Spindle assembly is subject to the regulatory controls of both cell-cycle machinery and Ran-signaling pathway. An important question how two pathways communicate with each other achieve coordinated regulation in mitosis. We show here that Cdc2 kinase phosphorylates serines located or near nuclear localization signal (NLS) human RCC1, nucleotide exchange factor for Ran. This phosphorylation necessary RCC1 generate RanGTP on mitotic chromosomes mammalian cells, which turn required spindle...
The genome-wide chromosome conformation capture (Hi-C) has revealed that the eukaryotic genome can be partitioned into A and B compartments have distinctive chromatin transcription features. Current Principle Component Analyses (PCA)-based method for A/B compartment prediction based on Hi-C data requires substantial CPU time memory. We report development of a method, CscoreTool, which enables fast memory-efficient determination at high resolution even in datasets with low sequencing...
Although studies suggest that perturbing mitotic progression leads to DNA damage and p53 activation, which in turn lead either cell apoptosis or senescence, it remains unclear how defects trigger activation. We show BuGZ Bub3, are two regulators localized the interphase nucleus, interact with splicing machinery required for pre-mRNA splicing. Similar inhibition of RNA by pladienolide B, depletion Bub3 led increased formation RNA–DNA hybrids (R-loops), activation both human tumor cells...
In mammalian cell nuclei, the nuclear lamina (NL) underlies envelope (NE) to maintain structure. The lamins, major structural components of NL, are involved in protection against NE rupture induced by mechanical stress. However, specific role lamins repair ruptures has not been fully determined. Our analyses using immunofluorescence and live-cell imaging revealed that nucleoplasmic pool lamin C rapidly accumulated at sites laser microirradiation mouse embryonic fibroblasts. accumulation...
Lamins, the major structural components of nuclear lamina (NL) found beneath envelope, are known to interact with most peripheral chromatin in metazoan cells. Although NL–chromatin associations correlate a repressive state, role lamins tethering NL and how such tether influences gene expression have remained challenging decipher. Studies suggest that proteins regulate context-dependent manner. Therefore understanding context states based on genomic features, including chromatin–NL...
The RanGTP gradient across the interphase nuclear envelope and on condensed mitotic chromosomes is essential for many cellular processes, including nucleocytoplasmic transport spindle assembly. Although chromosome-associated enzyme RCC1 responsible production, mechanism of generating maintaining in vivo remains unknown. Here, we report that regulator chromosome condensation (RCC1) rapidly associates dissociates with both living cells, this mobility regulated during cell cycle. Our kinetic...
Abstract To understand cell fate specification and maintenance during development, it is essential to visualize both lineage markers behaviors in real time using endogenous report fate. We have generated a reporter line which eGFP fused the locus of Cdx2 , transcription factor for trophectoderm specification, allowing us decisions preimplantation mouse embryo. used two‐photon laser scanning microscopy expression fusion protein show that undergoes phases upregulation. Additionally, we as late...
Previous work identified a γ-tubulin gene, γTub23C,inDrosophila(Zhenget al.,1991). We now report identification of second γTub37CD.Immunoblot analysis and immunolocalization show that γTub37CD γTub23C are differentially expressed during gametogenesis development. During oogenesis, was detected at centrosomes in the cytoplasm mitotic germ cells, but not cells following completion mitosis. Conversely, proliferating appeared to accumulate egg chamber Neither isoform anterior or posterior poles...