A5064852003- Plant Molecular Biology Research
- RNA modifications and cancer
- Plant Disease Resistance and Genetics
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Bryophyte Studies and Records
- Plant nutrient uptake and metabolism
- Genomics and Phylogenetic Studies
- Plant Reproductive Biology
- Plant Stress Responses and Tolerance
- Botany and Plant Ecology Studies
- Wheat and Barley Genetics and Pathology
- Rice Cultivation and Yield Improvement
- Photosynthetic Processes and Mechanisms
- Chromosomal and Genetic Variations
- Plant Genetic and Mutation Studies
- Plant-Microbe Interactions and Immunity
- Lichen and fungal ecology
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Cancer-related molecular mechanisms research
- Plant Gene Expression Analysis
- Plant Pathogens and Resistance
- MicroRNA in disease regulation
- Genomics and Chromatin Dynamics
Adam Mickiewicz University in Poznań
2016-2025
Institute of Molecular Biology and Biotechnology
2008-2023
James Hutton Institute
2013
University of Dundee
2009-2013
Institute of Molecular Biology
2013
Biomathematics and Statistics Scotland
2013
Max Planck Innovation
2009
Max Planck Society
2009
Laboratoire d'Enzymologie et Biochimie Structurales
1994
Centre National de la Recherche Scientifique
1994
MicroRNAs are the key post-transcriptional regulators of gene expression in development and stress responses. Thus, precisely quantifying level each particular microRNA is utmost importance when studying biology any organism. The mirEX 2.0 web portal ( http://www.combio.pl/mirex ) provides a comprehensive platform for exploration data based on quantitative Real Time PCR NGS sequencing experiments, covering various developmental stages, from wild-type to mutant plants. includes mature...
Arabidopsis microRNA expression regulation was studied in a wide array of abiotic stresses such as drought, heat, salinity, copper excess/deficiency, cadmium excess, and sulfur deficiency. A home-built RT-qPCR mirEX platform for the amplification 289 transcripts used to study their response stresses. Small RNA sequencing, Northern hybridization, TaqMan® assays were performed abundance mature microRNAs. broad on level primary miRNAs (pri-miRNAs) observed. However, stress at microRNAs rather...
We identified a human orthologue of tRNA:m5C methyltransferase from Saccharomyces cerevisiae, which has been previously shown to catalyse the specific modification C34 in intron-containing yeast |${\hbox{ pre-tRNA }}_{\left(\hbox{ CAA }\right)}^{\hbox{ Leu }}$|. Using transcripts intron-less and tRNA }}$| genes as substrates, we have that m5C34 is introduced only precursors when substrates were incubated HeLa extract. formation depends on nucleotide sequence surrounding wobble cytidine...
Heat stress is one of the major abiotic factors that can induce severe plant damage, leading to a decrease in crop productivity. Despite barley being cereal great economic importance, few data are available concerning its thermotolerance mechanisms. In this work microRNAs (miRNAs) involved heat response were investigated. The level selected mature miRNAs was examined by hybridization. Quantitative real-time PCR (RT-qPCR) used monitor changes expression profiles primary miRNA (pri-miRNA)...
Summary Developing new strategies for crop plants to respond drought is crucial their innovative breeding. The down‐regulation of nuclear cap‐binding proteins in A rabidopsis renders tolerant. CBP 80 gene the potato cultivar Desiree was silenced using artificial micro RNA s. Transgenic displayed a higher tolerance drought, ABA ‐hypersensitive stomatal closing, an increase leaf stomata and trichome density, compact cuticle structures with lower number microchannels. These findings were...
Arabidopsis thaliana HYL1 is a nuclear double-stranded RNA-binding protein involved in the maturation of pri-miRNAs. A quantitative real-time PCR platform for parallel quantification 176 pri-miRNAs was used to reveal strong accumulation 57 miRNA precursors hyl1 mutant that completely lacks protein. This approach enabled us first time pinpoint particular members MIRNA family genes require activity efficient their precursors. Moreover, gave opportunity carry out 3′ and 5′ RACE experiments...
In Arabidopsis thaliana, the METTL3 homolog, mRNA adenosine methylase (MTA) introduces N6-methyladenosine (m6A) into various coding and noncoding RNAs of plant transcriptome. Here, we show that an MTA-deficient mutant (mta) has decreased levels microRNAs (miRNAs) but accumulates primary miRNA transcripts (pri-miRNAs). Moreover, pri-miRNAs are methylated by MTA, RNA structure probing analysis reveals a decrease in secondary within stem-loop regions these mta plants. We demonstrate interaction...
Plant MIR genes are independent transcription units that encode long primary miRNA precursors, which usually contain introns. For two genes, MIR163 and MIR161 , we show introns crucial for the accumulation of proper levels mature miRNA. Removal intron in both cases led to a drop‐off level miRNAs. We demonstrate stimulating effects mostly reside 5′ss rather than on genuine splicing event. Our findings biologically significant as presence functional splice sites gene appears mandatory...
In most organisms, the maturation of nascent RNAs is coupled to transcription. Unlike in animals, RNA polymerase II (RNAPII) transcribes microRNA genes (MIRNAs) as long and structurally variable pri-miRNAs plants. Current evidence suggests that miRNA biogenesis complex assembly initiates early during transcription However, it unknown whether processing occurs co-transcriptionally. Here, we used native elongating transcript sequencing data imaging techniques demonstrate plant We found entire...
Accurate and comprehensive annotation of transcript sequences is essential for quantification differential gene expression analysis. Single-molecule long-read sequencing technologies provide improved integrity structures including alternative splicing, transcription start polyadenylation sites. However, accuracy significantly affected by errors, mRNA degradation, or incomplete cDNA synthesis.We present a new Arabidopsis thaliana Reference Transcript Dataset 3 (AtRTD3). AtRTD3 contains over...
Interferons (IFNs) induce gene expression by phosphorylating latent transcription factors belonging to the signal transducer and activator of (STAT) family, mediated janus kinases (Jaks). STAT dimers directly activate genes containing IFNgamma activation site (GAS) DNA element, with different proteins displaying slightly intrinsic binding specificities. The combinatorial association STATs additional adaptor protein interferon regulatory factor (IRF)9 expands range enhancer elements that can...
The nuclear cap-binding protein complex (CBC) participates in 5' splice site selection of introns that are proximal to the mRNA cap. However, it is not known whether CBC has a role alternative splicing. Using an RT-PCR splicing panel, we analysed 435 events Arabidopsis thaliana genes, encoding mainly transcription factors, factors and stress-related proteins. Splicing profiles were determined wild type plants, cbp20 cbp80(abh1) single mutants cbp20/80 double mutant. included 3' selection,...
How alternative splicing (AS) is regulated in plants has not yet been elucidated. Previously, we have shown that the nuclear cap-binding protein complex (AtCBC) involved AS Arabidopsis thaliana. Here show both subunits of AtCBC (AtCBP20 and AtCBP80) interact with SERRATE (AtSE), a microRNA biogenesis pathway. Moreover, using high-resolution reverse transcriptase-polymerase chain reaction system found AtSE influences similar way to (CBC), preferentially affecting selection 5′ splice site...
Plants as sessile organisms have developed prompt response mechanisms to react rapid environmental changes. In addition the transcriptional regulation of gene expression, microRNAs (miRNAs) are key posttranscriptional regulators plant stress response. We show here that expression levels many miRNAs were regulated under salt conditions. This occurred at and levels. During salinity stress, miRNA161 miRNA173 increased, while pri-miRNA161 pri-miRNA173 was down-regulated. Under conditions,...
Summary Liverworts are the most basal group of extant land plants. Nonetheless, molecular biology liverworts is poorly understood. Gene expression has been studied in only one species, Marchantia polymorpha . In particular, no microRNA (miRNA) sequences from have reported. Here, Illumina‐based next‐generation sequencing was employed to identify small RNAs, and analyze transcriptome degradome Pellia endiviifolia Three hundred eleven conserved miRNA plant families were identified, 42 new...
Several genes encoding transcription factors (TFs) were indicated to have a key role in the induction of somatic embryogenesis (SE), which is triggered cells plants. In order further explore genetic regulatory network that involved embryogenic transition induced plant cells, micro-RNA (miRNAs) molecules, products MIRNA (MIR) and common regulators TF transcripts, analysed an culture Arabidopsis thaliana. total, expression 190 114 families was monitored during SE levels primary (pri-miRNAs)...
Arabidopsis, miR402 that is encoded within the first intron of a protein-coding gene At1g77230, induced by heat stress. Its upregulation correlates with splicing inhibition and intronic proximal polyA site selection. It suggests not processed from an intron, but rather shorter transcript after selection this intron. Recently, introns active 5΄ splice sites (5΄ss') have been shown to stimulate accumulation miRNAs exons intron-containing MIR genes. In contrast, we observed opposite effect on...
Phosphorus (P) in plants is taken from soil as an inorganic phosphate (Pi) and one of the most important macroelements growth development. Plants actively react to Pi starvation by induced expression transporters, MIR399, MIR827, miR399 molecular sponge - IPS1 genes decreased ubiquitin-conjugating enzyme E2 (PHOSPHATE2 PHO2) sensing transport SPX-MFS genes. The PHO2 protein involved degradation transporters PHT1;1 (from roots) PHO1 roots shoots). leads accumulation shoots. In contrast, pho1...
Abstract The physiological responses of potato ( Solanum tuberosum L) cultivars to soil drought at the tuberization phase and their impact on agronomically important traits were investigated in potted plants under semi‐controlled conditions. Genotype‐dependent water deficiency evaluated two pairs (tolerant/sensitive) Polish cultivars. Each pair had a similar genetic background but was extremely different terms tolerance basis loss tuber yield field results clearly indicate mechanisms ability...
Abstract SERRATE/ARS2 is a conserved RNA effector protein involved in transcription, processing and export of different types RNAs. In Arabidopsis, the best-studied function SERRATE (SE) to promote miRNA processing. Here, we report that SE interacts with nuclear exosome targeting (NEXT) complex, comprising helicase HEN2, binding RBM7 one two zinc-knuckle proteins ZCCHC8A/ZCCHC8B. The identification common targets HEN2 by RNA-seq supports idea cooperates NEXT for surveillance exosome. Among...
Drought is one of the main climate threats limiting crop production. Potato four most important food species worldwide and sensitive to water shortage. The CBP80 gene was shown affect Arabidopsis potato responses drought by regulating level microRNA159 and, consequently, levels MYB33 MYB101 transcription factors (TFs). Here, we show that three MYB TFs, MYB33, MYB65, MYB101, are involved in plant Their downregulation causes stomatal hyposensitivity abscisic acid (ABA), leading reduced...
MicroRNAs (miRNAs) are major regulators of gene expression during plant development under normal and stress conditions. In this study, we analyzed the 150 conserved miRNAs drought applied to barley ready flower. The dynamics was also observed after rewatering. Target messenger RNA (mRNAs) were experimentally identified for all but two miRNAs, 41 targets not reported before. Drought induced accelerated flowering coordinated by a pair differently expressed originating from single precursor:...