A5083849566- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant Virus Research Studies
- Plant Reproductive Biology
- Plant and Fungal Interactions Research
- Light effects on plants
- Plant Disease Resistance and Genetics
- Computational Drug Discovery Methods
- Plant nutrient uptake and metabolism
- Cancer, Hypoxia, and Metabolism
- RNA Interference and Gene Delivery
- Metabolomics and Mass Spectrometry Studies
- HER2/EGFR in Cancer Research
- Fibroblast Growth Factor Research
- Natural product bioactivities and synthesis
- Plant biochemistry and biosynthesis
- Chromosomal and Genetic Variations
- Plant Gene Expression Analysis
- Epigenetics and DNA Methylation
- Plant-Microbe Interactions and Immunity
- Plant Stress Responses and Tolerance
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- Cancer-related Molecular Pathways
- Lipid metabolism and biosynthesis
Martin Luther University Halle-Wittenberg
2010-2025
Czech Academy of Sciences, Institute of Computer Science
2024-2025
Luther University
2024
Functions for RNA-binding proteins in orchestrating plant development and environmental responses are well established. However, the lack of a genome-wide view their vivo binding targets landscapes represents gap understanding mode action proteins. Here, we adapt individual nucleotide resolution crosslinking immunoprecipitation (iCLIP) to determine repertoire circadian clock-regulated Arabidopsis thaliana glycine-rich protein AtGRP7.iCLIP identifies 858 transcripts with significantly...
Abstract The hnRNP-like glycine-rich RNA-binding protein AtGRP7 regulates pre-mRNA splicing in Arabidopsis. Here we used small RNA-seq to show that also affects the miRNA inventory. overexpression caused a significant reduction level of 30 miRNAs and an increase for 14 with minimum log2 fold change ±0.5. Overaccumulation several pri-miRNAs including pri-miR398b, pri-miR398c, pri-miR172b, pri-miR159a pri-miR390 at expense mature suggested pri-miRNA processing. Indeed, RNA immunoprecipitation...
Abstract RNA-mediated crop protection increasingly becomes a viable alternative to agrochemicals that threaten biodiversity and human health. Pathogen-derived double-stranded RNAs (dsRNAs) are processed into small interfering (siRNAs), which can then induce silencing of target RNAs, e.g. viral genomes. However, with currently used dsRNAs, largely consist undefined regions the is often ineffective: processing in plant generates siRNA pools contain only few functionally effective siRNAs...
Rapeseed (Brassica napus L.), one of the most important sources vegetable oil and protein-rich meals worldwide, is adapted to different geographical regions by modification flowering time. cultivars have day length vernalization requirements, which categorize them into winter, spring, semiwinter ecotypes. To gain a deeper insight genetic factors controlling floral transition in B. napus, we performed RNA sequencing (RNA-seq) doubled haploid line, Ningyou7, at developmental stages temperature...
In response to a viral infection, the plant's RNA silencing machinery processes RNAs into huge number of small interfering (siRNAs). However, very few these siRNAs actually interfere with replication. A reliable approach identify immunologically effective (esiRNAs) and define characteristics underlying their activity has not been available so far. Here, we develop novel screening that enables rapid functional identification antiviral esiRNAs. Tests on efficacy such identified esiRNAs model...
ABSTRACT Many viral suppressors (VSRs) counteract antiviral RNA silencing, a central component of the plant’s immune response by sequestration virus-derived small interfering RNAs (siRNAs). Here, we addressed how VSRs affect activities cellular microRNAs (miRNAs) during infection characterizing interactions two unrelated VSRs, Tombusvirus p19 and Cucumovirus 2b, with miRNA 162 (miR162), miR168, miR403. These miRNAs regulate expression important silencing factors Dicer-like protein 1 (DCL1)...
Abstract Auxin plays a pivotal role in virtually every aspect of plant morphogenesis. It simultaneously orchestrates diverse variety processes such as cell wall biogenesis, transition through the cycle, or metabolism wide range chemical substances. The coordination principles for complex orchestration are poorly understood at systems level. Here, we perform an RNA-seq experiment to study transcriptional response auxin treatment within gene groups different biological processes, molecular...
The transcription factor hypoxia-inducible 1 (HIF1) is the crucial regulator of genes that are involved in metabolism under hypoxic conditions, but information regarding transcriptional activity HIF1 normoxic limited. Different tumor cells were treated and conditions with various drugs affect cellular metabolism. HIF1α was silenced by siRNA normoxic/hypoxic cells, before RNA sequencing bioinformatics analyses performed while using breast cancer cell line MDA-MB-231 as a model. Differentially...
The epidermal growth factor receptor (EGFR) is a major regulator of proliferation in tumor cells. Elevated expression levels EGFR are associated with prognosis and clinical outcomes patients variety types. There at least four splice variants the mRNA encoding protein isoforms humans, named I through IV. isoform full-length protein, whereas II-IV shorter isoforms. Nevertheless, all bind (EGF). Although an essential target long-established successful therapeutics, exact function biomarker...
Abstract RNA-mediated crop protection increasingly becomes a viable alternative to agrochemicals that threaten biodiversity and human health. Pathogen-derived double-stranded dsRNAs are processed into small interfering RNAs (siRNAs), which can then induce silencing of target RNAs, e.g. viral genomes. However, with currently used dsRNAs, largely consist undefined regions the is often ineffective: processing generates siRNA pools contain only few functionally effective siRNAs (here called e...
Betulinic acid (BA) is a natural compound well known for its anti-inflammatory, anti-viral, anti-bacterial, anti-malarial effects and anti-tumor properties. Its enhanced cytotoxicity in tumor cells induction of cell death various cancer entities qualifies BA as an interesting candidate novel treatment concepts. Our analyses showed radiosensitization under hypoxic conditions human breast cells. So far, the underlying mechanisms are unknown. Therefore, we investigated BA-treated lines...
Abstract The historic developmental hourglass concept depicts the convergence of animal embryos to a common form during phylotypic period. Recently, it has been shown that transcriptomic is associated with this morphological pattern, consistent idea underlying selective constraints due intense molecular interactions body plan establishment. Although plants do not exhibit embryogenesis, nevertheless identified in model plant Arabidopsis thaliana . Here, we investigated whether patterns are...