A5065956430- Sarcoma Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Cancer-related Molecular Pathways
- Cancer Research and Treatments
- RNA Interference and Gene Delivery
- Cell death mechanisms and regulation
- Enzyme function and inhibition
- Bone and Dental Protein Studies
- Natural product bioactivities and synthesis
- Cancer-related molecular mechanisms research
- Virus-based gene therapy research
- Molecular Biology Techniques and Applications
- Cancer Cells and Metastasis
- Glioma Diagnosis and Treatment
- Plant biochemistry and biosynthesis
- Cancer Genomics and Diagnostics
- DNA Repair Mechanisms
- Neuroblastoma Research and Treatments
- Epigenetics and DNA Methylation
- Protease and Inhibitor Mechanisms
- Tumors and Oncological Cases
- ATP Synthase and ATPases Research
- Neurofibromatosis and Schwannoma Cases
- Colorectal and Anal Carcinomas
- Heat shock proteins research
Martin Luther University Halle-Wittenberg
2015-2024
University Hospital in Halle
2014-2021
Luther University
2013
Wittenberg University
1997-2005
Hypoxia induces activation of the HIF-1 pathway and is an essential characteristic malignant gliomas. has been linked to tumor progression, therapy resistance poor prognosis. However, little known about impact HIF-1α inhibition on radioresistance glioma.In this study, we investigated effects cell survival radiosensitivity in U251MG U343MG glioma cells, using two different strategies. was achieved by siRNA targeting or via chetomin, a disruptor interactions between p300. The monitored...
Survivin, a recently identified inhibitor of apoptosis protein (IAP), is expressed in diverse embryonic tissues and various human cancers. We have investigated the quantitative expression survivin mRNA by sensitive TaqMan-based RT-PCR assay tissue samples from 94 patients with soft sarcomas (STS). Survivin transcript levels were measured normalized to GAPDH transcripts. By using multivariate Cox regression analysis, we found an inverse correlation between level (ratio >2 zmol survivin/amol...
The product of the HDMX (or MDM4) gene is structurally related to MDM2 oncoprotein and also capable interacting with tumor suppressor protein p53. aim our study was determine amplification status expression mRNA (particularly that HDMX-S splice variant) in soft-tissue sarcomas (STS). Patients STS were evaluated for (n = 66) 57) within their tumors. DNA, total RNA isolated from frozen tissue. We determined variant transcript predominant a subset (14%) samples its correlated decreased patient...
We investigated 31 malignant peripheral nerve sheath tumors (MPNSTs) from 23 patients by means of comparative genomic hybridization (CGH) in order to study quantitative aberrations these tumors. Twenty-one the revealed changes, with a mean value 11 per sample (range 2–29). The minimal common regions most frequent gains were 8q23-q24.1 (12 cases), 5p14 (11 and 6p22-pter, 7p15-p21, 7q32-q35, 8q21.1-q22, 8q24.2-qter, 17q22-qter (10 cases each). Seventeen high-level amplifications detected eight...
Expression of microRNAs can affect age tumor onset and prognosis cancer patients. However, nothing is known about the effects on altered disease-specific survival soft-tissue sarcoma (STS) The levels miR-210, also as hypoxia-regulated microRNA, were analyzed by quantitative real-time (RT)-PCR in tumors 78 STS patients stratified according to their microRNA with low, intermediate high expression association patients' was using multivariate Cox's regression hazard analyses. A significant...
Hypoxia mediates resistance to radio(chemo)therapy (RT) by stimulating the synthesis of hypoxia-related genes, such as osteopontin (OPN) and stress proteins, including major stress-inducible heat shock protein 70 (Hsp70). Apart from its intracellular localization, Hsp70 is also present on plasma membrane viable tumor cells that actively release it in lipid vesicles with biophysical characteristics exosomes. Exosomal contributes radioresistance while derived dying can serve a stimulator...
In contrast to normal cells, tumor cells of multiple entities overexpress the Heat shock protein 70 (Hsp70) not only in cytosol, but also present it on their plasma membrane a tumor-specific manner. Furthermore, Hsp70-positive actively release Hsp70 small extracellular vesicles with biophysical characteristics exosomes. Due conformational changes lipid environment, most commercially available antibodies fail detect membrane-bound and vesicular Hsp70. To fill this gap assess role circulation...
1,5-Diazacyclooctane was prepared by a simple synthetic sequence and coupled to pentacyclic triterpenoic acids oleanolic acid, ursolic betulinic platanic asiatic acid; these amides were activated with oxalyl chloride reacted rhodamine B or 101 yield conjugates. The conjugates screened in SRB assays various human breast cancer cell lines (MDA-MB-231, HS578T, MCF-7, T47D) found exert cytotoxic activity even at low concentration. Therefore, for an acid conjugate (28), IC50 = 0.60 nM determined...
The MDM2 gene encodes a 90-kDa oncoprotein that is overexpressed in several human carcinomas, osteosarcomas, gliomas and soft tissue sarcomas (STSs). This overexpression the result of mechanisms, for example, enhanced transcription or translation, amplification alternative splicing. We found 19 67 (28.4%) STS specimens contained an amplified gene. was more likely to be present grade 1 tumors than 2 3 (58% vs. 15% tumors, p = 0.001, chi(2) test). Furthermore, patients with had survival...
The tumor-associated glycoprotein osteopontin (OPN) is discussed as a plasma surrogate marker of tumor hypoxia and an indicator the presence pleural mesothelioma in asbestos-exposed individuals. clinical introduction OPN measurements requires availability reliable enzyme-linked immunosorbence assay (ELISA). We compared previously described currently available ELISA systems on 88 archival samples obtained from patients with head neck or cervix cancer between 20 days before 171 after start...
This study investigates the prognostic impact of expression hypoxia-inducible factor 1alpha (Hif1alpha) and carbonic anhydrase IX (CAIX) detected by immunohistochemistry in oral squamous cell carcinoma (OSCC).Statistical analysis immunohistochemical results with clinical parameters including survival outcomes was performed for 80 OSCC patients.Patients a low both proteins survived on average 54.8 months, whereas those an increased Hif1alpha their tumors combined CAIX only 37.6 months (P =...
The urokinase plasminogen activator (uPA) system is one of the best-investigated protease systems, both under physiological and pathological conditions, including various types cancer. However, effects co-expression members uPA in soft-tissue sarcoma (STS) patients at protein level tumour tissue serum have not been investigated yet. We examined 82 STS for levels uPA, PAI-1and uPAR by ELISA. A significant correlation between high antigen PAI-1 or tissue, serum, with poor outcome was found...
Betulinic acid (BA) is a novel antineoplastic agent under evaluation for tumor therapy. Because of the selective cytotoxic effects BA in cells (including gliomas), combination this with conservative therapies (such as radiotherapy and chemotherapy) may be useful. Previously, irradiation hypoxic conditions had never been studied. In study, 3 to 30 μM on cytotoxicity, migration, protein expression PARP, survivin HIF-1α, well radiosensitivity normoxic were analyzed human malignant glioma cell...