A5029069646- Seed Germination and Physiology
- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant Genetic and Mutation Studies
- Soybean genetics and cultivation
- Photosynthetic Processes and Mechanisms
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Genetic Mapping and Diversity in Plants and Animals
- Plant Pathogens and Resistance
- Plant Gene Expression Analysis
- Plant Water Relations and Carbon Dynamics
- Plant Reproductive Biology
- Genomics and Phylogenetic Studies
- Genetics and Plant Breeding
- Bioinformatics and Genomic Networks
- Legume Nitrogen Fixing Symbiosis
- Growth and nutrition in plants
- GABA and Rice Research
- Plant Pathogens and Fungal Diseases
- Gene expression and cancer classification
- Horticultural and Viticultural Research
- Plant responses to water stress
- Agriculture, Plant Science, Crop Management
Wageningen University & Research
2015-2024
Graduate School Experimental Plant Sciences
2003-2021
Institut de recherche en horticulture et semences
2015
University of Groningen
2011
Institut des Sciences des Plantes de Montpellier
2010
Vienna Biocenter
1996-2001
University of Vienna
1996-2001
Leibniz Institute of Plant Biochemistry
1997
Yeast and animals use mitogen-activated protein (MAP) kinase cascades to mediate stress extracellular signals. We have tested whether MAP kinases are involved in mediating environmental responses plants. Using specific peptide antibodies that were raised against different alfalfa kinases, we found exclusive activation of p44MMK4 drought- cold-treated was transiently activated by these treatments correlated with a shift the electrophoretic mobility protein. Although transcript levels MMK4...
Over the past few decades seed physiology research has contributed to many important scientific discoveries and provided valuable tools for production of high quality seeds. An instrument this type is accurate quantification germination; however gathering cumulative germination data a very laborious task that often prohibitive execution large experiments. In paper we present germinator package: simple, highly cost-efficient flexible procedure high-throughput automatic scoring evaluation can...
Quantifying gene expression levels is an important research tool to understand biological systems. Reverse transcription-quantitative real-time PCR (RT-qPCR) the preferred method for targeted measurements because of its sensitivity and reproducibility. However, normalization, necessary correct sample input reverse transcriptase efficiency, a crucial step obtain reliable RT-qPCR results. Stably expressed genes (i.e. whose not affected by treatment or developmental stage under study) are...
Summary Plants are exposed to combinations of various biotic and abiotic stresses, but stress responses usually investigated for single stresses only. Here, we the genetic architecture underlying plant 11 several their by phenotyping 350 Arabidopsis thaliana accessions. A set 214 000 nucleotide polymorphisms ( SNP s) was screened marker‐trait associations in genome‐wide association GWA ) analyses using tailored multi‐trait mixed models. Stress that share phytohormonal signaling pathways also...
Abstract Microbiomes play a pivotal role in plant growth and health, but the genetic factors involved microbiome assembly remain largely elusive. Here, we map molecular features of rhizosphere as quantitative traits diverse hybrid population wild domesticated tomato. Gene content analysis prioritized tomato trait loci suggests basis for differential recruitment various rhizobacterial lineages, including Streptomyces -associated 6.31 Mbp region harboring domestication sweeps encoding, among...
Parsley cells recognize the fungal plant pathogen Phytophthora sojae through a plasma membrane receptor. A pathogen-derived oligopeptide elicitor binds to this receptor and thereby stimulates multicomponent defense response sequential activation of ion channels an oxidative burst. An elicitor-responsive mitogen-activated protein (MAP) kinase was identified that acts downstream but independently or upstream Upon receptor-mediated activation, MAP is translocated nucleus where it might interact...
Mechanical injury in plants induces responses that are involved not only healing but also defense against a potential pathogen. To understand the intracellular signaling mechanism of wounding, we have investigated involvement protein kinases. Using specific antibodies, showed wounding alfalfa leaves specifically transient activation p44MMK4 kinase, which belongs to family mitogen-activated Whereas MMK4 pathway is post-translational process and was blocked by [alpha]-amanitin cycloheximide,...
The combination of robust physiological models with "omics" studies holds promise for the discovery genes and pathways linked to how organisms deal drying. Here we used a transcriptomics approach in an vivo model re-establishment desiccation tolerance (DT) Arabidopsis thaliana seeds. We show that incubation sensitive (DS) germinated seeds polyethylene glycol (PEG) solution re-induces mechanisms necessary expression DT. Based on SNP-tile array gene profile, our data indicates DT, this system,...
Late embryogenesis abundant (LEA) proteins include eight multigene families that are expressed in response to water loss during seed maturation and vegetative tissues of desiccation tolerant species. To elucidate LEA evolution diversification, we performed a comprehensive synteny phylogenetic analyses the gene across 60 complete plant genomes. Our integrated comparative genomic approach revealed conservation diversification contributed family expansion functional plants. We provide examples...
Summary Plant growth is severely affected by hyper‐osmotic salt conditions. Although a number of salt‐induced genes have been isolated, the sensing and signal transduction stress little understood. We provide evidence that alfalfa cells two osmo‐sensing protein kinase pathways are able to distinguish between moderate extreme A 46 kDa was found be activated elevated concentrations (above 125 m NaCl). In contrast, at high 750 NaCl), 38 kinase, but not became activated. By biochemical...
Summary The heterotrimeric G‐protein pathway is a ubiquitous eukaryotic signalling module that known to regulate growth and differentiation in many plant pathogens. We previously identified Pigpa1 , gene encoding α subunit from the potato late blight pathogen Phytophthora infestans. P. infestans belongs class oomycetes, group of organisms which signal transduction processes have not yet been studied at molecular level. To elucidate function PiGPA1‐deficient mutants were obtained by...
Much of the pathogenic success Phytophthora infestans, potato and tomato late blight agent, relies on its ability to generate from mycelia large amounts sporangia, which release zoospores that encyst form infection structures. To better understand these stages, Affymetrix GeneChips based 15,650 unigenes were designed used profile life cycle. Approximately half P. infestans genes found exhibit significant differential expression between developmental transitions, with approximately (1)/(10)...
Summary During germination, orthodox seeds lose their desiccation tolerance ( DT ) and become sensitive to extreme drying. Yet, can be rescued, in a well‐defined developmental window, by the application of mild osmotic stress before dehydration. A role for abscisic acid ABA has been implicated this response re‐establishment. However, path from sensing an cue its signaling re‐establishment is still largely unknown. Analyses , sensitivity, content gene expression were performed...
The success of germination, growth and final yield every crop depends to a large extent on the quality seeds used grow crop. Seed is defined as viability vigor attribute seed that enables emergence establishment normal seedlings under wide range environments. We attempt dissect mechanisms involved in acquisition quality, through combined approach physiology genetics. To achieve this goal we explored genetic variation found RIL population Solanum lycopersicum (cv. Moneymaker) x...
The transition from a quiescent dry seed to an actively growing photoautotrophic seedling is complex and crucial trait for plant propagation. This study provides detailed description of global gene expression in seven successive developmental stages establishment Arabidopsis (Arabidopsis thaliana). Using the transcriptome signature these stages, we obtained coexpression network that highlights interactions between known regulators seed-to-seedling predicts functions uncharacterized genes...
Compared with major crops, growth and development of Ricinus communis is still poorly understood. A better understanding the biochemical physiological aspects germination seedling crucial for breeding high yielding varieties adapted to various growing environments. In this context, we analysed effect temperature on young R. seedlings measured primary secondary metabolites in roots cotyledons. Three genotypes, recommended small family farms as cash crop, were used study.Seedling biomass was...
Sweetpotato yield depends on a change in the developmental fate of adventitious roots into storage-roots. The mechanisms underlying this switch are still unclear. We examined hypothesis claiming that regulation root lignification determines storage-root formation. show application plant hormone gibberellin increased stem elongation and levels, while having inhibitory effects system parameters, decreasing lateral number length, significantly reducing diameter. Furthermore, enhanced xylem...
Abstract A complex phenotype such as seed germination is the result of several genetic and environmental cues requires concerted action many genes. The use well-structured recombinant inbred lines in combination with “omics” analysis can help to disentangle basis quantitative traits. This so-called genetical genomics approach effectively capture both epistatic interactions. However, understand how environment interacts genomic-encoded information, a better understanding perception processing...