A5019725092- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Genomics and Phylogenetic Studies
- Metabolomics and Mass Spectrometry Studies
- Microbial Metabolic Engineering and Bioproduction
- Polysaccharides and Plant Cell Walls
- Agronomic Practices and Intercropping Systems
- Biofuel production and bioconversion
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Chromatography
- Cell Image Analysis Techniques
- Nematode management and characterization studies
- Advanced Proteomics Techniques and Applications
- Polysaccharides Composition and Applications
- Bacterial Genetics and Biotechnology
- Plant Molecular Biology Research
- Coastal wetland ecosystem dynamics
- Plant Reproductive Biology
- Plant Disease Resistance and Genetics
- Bacteriophages and microbial interactions
- Plant biochemistry and biosynthesis
Bielefeld University
2016-2025
Faculty (United Kingdom)
2016
Institut für biologische Forschung
2014
Leibniz University Hannover
2004-2012
Proteome Sciences (United Kingdom)
2011
Mississippi State University
2010
University of Bonn
2010
Palacký University Olomouc
2010
Consejo Superior de Investigaciones Científicas
2007
Universidad Nacional de La Plata
1992-2007
The genes MYB11, MYB12 and MYB111 share significant structural similarity form subgroup 7 of the Arabidopsis thaliana R2R3-MYB gene family. To determine regulatory potential these three transcription factors, we used a combination genetic, functional genomics metabolite analysis approaches. show high degree display very similar target specificity for several flavonoid biosynthesis, including CHALCONE SYNTHASE, ISOMERASE, FLAVANONE 3-HYDROXYLASE FLAVONOL SYNTHASE1. Seedlings triple mutant...
Innate immunity is based on the recognition of pathogen-associated molecular patterns (PAMPs). Here, we show that elongation factor Tu (EF-Tu), most abundant bacterial protein, acts as a PAMP in Arabidopsis thaliana and other Brassicaceae. EF-Tu highly conserved all bacteria known to be N-acetylated Escherichia coli. plants specifically recognize N terminus an peptide comprising first 18 amino acids, termed elf18, fully active inducer defense responses. The shorter peptide, elf12, acetyl...
The flavonol branch of flavonoid biosynthesis is under transcriptional control the R2R3-MYBs production glycoside1 (PFG1/MYB12, PFG2/MYB11 and PFG3/MYB111) in Arabidopsis thaliana. Here, we investigated influence specific PFG transcription factors on distribution various organs. A combination genetic metabolite analysis was used to identify factor gene-metabolite correlations metabolic pathway. Flavonol glycoside accumulation patterns have been analysed wild-type multiple R2R3-MYB mutants an...
Summary Interspecies signalling through the action of diffusible signal molecules can influence behaviour organisms growing in polymicrobial communities. Stenotrophomonas maltophilia and Pseudomonas aeruginosa occur ubiquitously environment be found together diverse niches including rhizosphere plants cystic fibrosis lung. In mixed species biofilms, S. substantially influenced architecture P. structures, which developed as extended filaments. This effect depended upon synthesis factor (DSF)...
Metagenomics, or the sequencing and analysis of collective genomes (metagenomes) microorganisms isolated from an environment, promises direct access to "unculturable majority". This emerging field offers potential lay solid basis on our understanding entire living world. However, taxonomic classification is essential task in metagenomics data sets that it still far being solved. We present a novel strategy predict origin environmental genomic fragments. The proposed classifier combines idea...
The polarization of sterol-enriched lipid microdomains has been linked to morphogenesis and cell movement in diverse types. Recent biochemical evidence confirmed the presence plant cells; however, direct for a functional link between these growth is still lacking. Here, we reported involvement NADPH oxidase (NOX)-dependent reactive oxygen species (ROS) signaling Picea meyeri pollen tube growth. Staining with di-4-ANEPPDHQ or filipin revealed that were polarized growing tip tube. Sterol...
Burkholderia cenocepacia is an opportunistic respiratory pathogen that can cause severe infections in immune-compromised individuals and associated with poor prognosis for patients suffering from cystic fibrosis. The second messenger cyclic diguanosine monophosphate (c-di-GMP) has been shown to control a wide range of functions bacteria, but little known about these regulatory mechanisms B. cenocepacia. Here we investigated the role c-di-GMP plays regulation biofilm formation virulence...
White lupin (Lupinus albus L.) is highly adapted to phosphorus-diminished soils. P-deficient white plants modify their root architecture and physiology acquire sparingly available soil phosphorus. We employed gas chromatography–mass spectrometry (GC MS) for metabolic profiling of lupins, investigate biochemical pathways involved in the P-acquiring strategy. After 14 days P-deficiency, showed reduced levels fructose, glucose sucrose shoots. Phosphorylated metabolites such as...
Outer membrane vesicles (OMVs) are released from the outer of many Gram-negative bacteria. These extracellular compartments known to transport compounds involved in cell-cell signalling as well virulence associated proteins, e.g. cytolysine enterotoxic E. coli.We have demonstrated that Xanthomonas campestris pv. (Xcc) releases OMVs into culture supernatant during growth. A proteome study identified 31 different proteins associate with OMV fraction which half virulence-associated. comparison...
In some European community countries up to 8% of the agricultural area is managed organically. The aim was obtain a metabolite profile for wheat (Triticum aestivum L.) grains grown under comparable organic and conventional conditions. These conditions cannot be found in plant material originating from different farms or products purchased supermarkets. Wheat long-term biodynamic, bioorganic, farming system harvest 2003 Switzerland were analyzed. presented data show that using high throughput...
Abstract In the establishment of symbiosis between Medicago truncatula and nitrogen-fixing bacterium Sinorhizobium meliloti, lipopolysaccharide (LPS) microsymbiont plays an important role as a signal molecule. It has been shown in cell cultures that LPS is able to suppress elicitor-induced oxidative burst. To investigate effect S. meliloti on defense-associated gene expression, microarray experiment was performed. For evaluation M. datasets, software tool MapMan, which initially developed...
Abstract Motivation: The recent advances in metabolomics have created the potential to measure levels of hundreds metabolites which are end products cellular regulatory processes. automation sample acquisition and subsequent analysis high-throughput instruments that capable measuring is posing a challenge on necessary systematic storage computational processing experimental datasets. Whereas multitude specialized software systems for individual preprocessing methods exists, there clearly...
Abstract RAC/ROP proteins (ρ-related GTPases of plants) are plant-specific small G that function as molecular switches within elementary signal transduction pathways, including the regulation reactive oxygen species (ROS) generation during early microbial infection via activation NADPH oxidase homologs plants termed RBOH (for respiratory burst homolog). We investigated role Medicago truncatula Jemalong A17 GTPase MtROP9, orthologous to sativa Rac1, an RNA interference silencing approach....
Abstract Motivation: The research area metabolomics achieved tremendous popularity and development in the last couple of years. Owing to its unique interdisciplinarity, it requires combine knowledge from various scientific disciplines. Advances high-throughput technology consequently growing quality quantity data put new demands on applied analytical computational methods. Exploration finally generated analyzed datasets furthermore relies powerful tools for mining visualization. Results: To...
Plant growth-promoting rhizobacteria (PGPR) are known to improve plant growth and used as biofertilizers, thanks their numerous benefits agriculture such phosphorus solubilization phytohormone production. In this paper, four rhizospheric bacteria (Phyllobacterium sp., Bacillus Agrobacterium Rhizobium sp.) isolated from surface-sterilized root nodules of Acacia cyanophylla were tested for ability solubilize inorganic phosphate produce indole-3-acetic acid (IAA) under laboratory conditions....
Summary A 2.6kb Clal‐Bam HI DNA fragment of megaplasmid 2 Rhizobium meliloti 2011 was found to carry genes involved in exopolysaccharide synthesis and infection alfalfa nodules. The analysis the nucleotide sequence this revealed existence two open reading frames (ORFs) running opposite directions. Plasmid integration mutagenesis showed that these ORFs are organized as monocistronic transcription units. One represents a new exo gene designated exoZ , which is in, but not essential for,...
Summary The membrane topology of the Rhizobium meliloti 2011 ExoP protein involved in polymerization and export succinoglycan was analysed by translational fusions lacZ phoA reporter genes to exoP gene. Based on this analysis, could be divided into an N‐terminal domain mainly located periplasmic space a C‐terminal cytoplasm. Whereas is characterized potential nucleotide‐binding motif, contains sequence motif‘PX 2 pX 4 SPKX 11 GXMXG 1 ′, which also present proteins determination O‐antigen...