A5055357299- Genomics and Phylogenetic Studies
- Microbial Natural Products and Biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Biofuel production and bioconversion
- Diphtheria, Corynebacterium, and Tetanus
- Microbial Community Ecology and Physiology
- Mycobacterium research and diagnosis
- Enzyme Production and Characterization
- Marine Sponges and Natural Products
- Bacteriophages and microbial interactions
- Plant Disease Resistance and Genetics
- Bacterial Identification and Susceptibility Testing
- Microbial Metabolism and Applications
- Plant Pathogenic Bacteria Studies
- RNA modifications and cancer
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Enzyme Structure and Function
- Probiotics and Fermented Foods
- Fungal Biology and Applications
- CRISPR and Genetic Engineering
- Streptococcal Infections and Treatments
- Plant Pathogens and Fungal Diseases
Bielefeld University
2016-2025
University of Münster
2009-2024
University Hospital Münster
2012-2024
University of Mannheim
2022-2023
Friedrich-Alexander-Universität Erlangen-Nürnberg
2017-2022
Massachusetts Institute of Technology
2015-2021
Singapore-MIT Alliance for Research and Technology
2021
Helmholtz Institute for Pharmaceutical Research Saarland
2020
Bioengineering Center
2017
National Center for Genetic Engineering and Biotechnology
2017
Cultivated bacteria such as actinomycetes are a highly useful source of biomedically important natural products. However, 'talented' producers represent only minute fraction the entire, mostly uncultivated, prokaryotic diversity. The uncultured majority is generally perceived large, untapped resource new drug candidates, but so far it unknown whether taxa containing talented indeed exist. Here we report single-cell- and metagenomics-based discovery producers. Two phylotypes candidate genus...
Understanding Mycobacterium tuberculosis (Mtb) transmission is essential to guide efficient control strategies. Traditional strain typing lacks sufficient discriminatory power resolve large outbreaks. Here, we tested the potential of using next generation genome sequencing for identification outbreak-related chains.During long-term (1997 2010) prospective population-based molecular epidemiological surveillance comprising a total 2,301 patients, identified outbreak caused by an Mtb Haarlem...
The genus Sorangium synthesizes approximately half of the secondary metabolites isolated from myxobacteria, including anti-cancer metabolite epothilone. We report complete genome sequence model strain S. cellulosum So ce56, which produces several natural products and has morphological physiological properties typical genus. circular genome, comprising 13,033,779 base pairs, is largest bacterial sequenced to date. No global synteny with Myxococcus xanthus apparent, revealing an unanticipated...
The gram-negative plant-pathogenic bacterium Xanthomonas campestris pv. vesicatoria is the causative agent of bacterial spot disease in pepper and tomato plants, which leads to economically important yield losses. This pathosystem has become a well-established model for studying infection strategies. Here, we present whole-genome sequence pepper-pathogenic strain 85-10, comprises 5.17-Mb circular chromosome four plasmids. genome high G+C content (64.75%) signatures extensive plasticity....
Abstract Background The use of RNAseq to resolve the transcriptional organization an organism was established in recent years and also showed complexity dynamics bacterial transcriptomes. aim this study comprehensively investigate transcriptome industrially relevant amino acid producer model Corynebacterium glutamicum by order improve its genome annotation describe important features for transcription translation. Results data sets were obtained two methods, one that focuses on 5′-ends...
ABSTRACT Clavibacter michiganensis subsp. is a plant-pathogenic actinomycete that causes bacterial wilt and canker of tomato. The nucleotide sequence the genome strain NCPPB382 was determined. chromosome circular, consists 3.298 Mb, has high G+C content (72.6%). Annotation revealed 3,080 putative protein-encoding sequences; only 26 pseudogenes were detected. Two rrn operons, 45 tRNAs, three small stable RNA genes found. two circular plasmids, pCM1 (27.4 kbp) pCM2 (70.0 kbp), which carry...
ABSTRACT The activity of bacteriophages and phage-related mobile elements is a major source for genome rearrangements genetic instability their bacterial hosts. the industrial amino acid producer Corynebacterium glutamicum ATCC 13032 contains three prophages (CGP1, CGP2, CGP3) so far unknown functionality. Several phage genes are regularly expressed, large prophage CGP3 (∼190 kbp) has recently been shown to be induced under certain stress conditions. Here, we present construction MB001,...
Abstract The prevalence of germ line mutations in non‐ BRCA 1/2 genes associated with hereditary breast cancer ( BC ) is low, and the role some these predisposition pathogenesis conflicting. In this study, 5589 consecutive index patients negative for pathogenic 2189 female controls were screened eight ATM , CDH 1 CHEK 2 NBN PALB RAD 51C 51D, TP 53 ). All met inclusion criteria German Consortium Hereditary Breast Ovarian Cancer testing. highest mutation was observed gene (2.5%), followed by...
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For synthetic biology applications, a robust structural basis is required, which can be constructed either from scratch or in top-down approach starting any existing organism. In this study, we initiated the construction of chassis organism Corynebacterium glutamicum ATCC 13032, aiming for relevant gene set to maintain its fast growth on defined medium. We evaluated each native essentiality considering expression levels, phylogenetic conservation, and knockout data. Based classification,...
Abstract Marine actinobacteria are drawing more and attention as a promising source of new natural products. Here we report isolation, genome sequencing metabolic profiling strain Streptomyces sp. MP131-18 isolated from marine sediment sample collected in the Trondheim Fjord, Norway. The 16S rRNA multilocus phylogenetic analysis showed that belongs to genus . isolate was sequenced, 36 gene clusters involved biosynthesis 18 different types secondary metabolites were predicted using antiSMASH...
Abstract The Hypoxylaceae (Xylariales, Ascomycota) is a diverse family of mainly saprotrophic fungi, which commonly occur in angiosperm-dominated forests around the world. Despite their importance forest and plant ecology as well prolific source secondary metabolites enzymes, genome sequences related taxa are scarce usually derived from environmental isolates. To address this lack knowledge thirteen taxonomically well-defined representatives one member closely Xylariaceae were sequenced...
Bacterial specialized metabolites are increasingly recognized as important factors in animal–microbiome interactions: for example, by providing the host with chemical defenses. Even chemically rich animals, such compounds have been found to originate from individual members of more diverse microbiomes. Here, we identified a remarkable case moderately complex microbiome sponge Mycale hentscheli which multiple symbionts jointly generate diversity. In addition bacterial pathways three distinct...
Abstract Non-obstructive azoospermia, the absence of sperm in ejaculate due to disturbed spermatogenesis, represents most severe form male infertility. De novo microdeletions Y-chromosomal AZFa region are one few well-established genetic causes for NOA and routinely analysed diagnostic workup affected men. So far, it is unclear which three genes located chromosomal indispensible germ cell maturation. Here we present four different likely pathogenic loss-of-function variants gene DDX3Y...
piRNAs are crucial for transposon silencing, germ cell maturation, and fertility in male mice. Here, we report on the genetic landscape of piRNA dysfunction humans present 39 infertile men carrying biallelic variants 14 different pathway genes, including PIWIL1, GTSF1, GPAT2, MAEL, TDRD1, DDX4. In some affected men, testicular phenotypes differ from those respective knockout mice range complete loss to production a few morphologically abnormal sperm. A reduced number pachytene was detected...
Abstract Background The maintenance of internal pH in bacterial cells is challenged by natural stress conditions, during host infection or biotechnological production processes. Comprehensive transcriptomic and proteomic analyses has been conducted several model systems, yet questions remain as to the mechanisms homeostasis. Results Here we present comprehensive analysis homeostasis C. glutamicum , a bacterium industrial importance. At values between 6 9 effective at 7.5 ± 0.5 units was...
Growing evidence suggests that a novel member of the Chlamydiales order, Waddlia chondrophila, is potential agent miscarriage in humans and abortion ruminants. Due to lack genetic tools manipulate chlamydia, genomic analysis proving be most incisive tool stimulating investigations into biology these obligate intracellular bacteria. 454/Roche Solexa/Illumina technologies were thus used sequence assemble de novo full genome first representative Waddliaceae family, W. chondrophila. The bacteria...
Abstract Glioblastoma multiforme (GBM) is an extremely malignant brain tumor. To identify new genomic alterations in GBM, DNA of tumor tissue/explants from 55 individuals and 6 GBM cell lines were examined using single nucleotide polymorphism microarray (SNP-Chip). Further gene expression analysis relied on additional 56 samples. SNP-Chip results validated several techniques, including quantitative PCR (Q-PCR), sequencing, a combination Q-PCR detection microsatellite markers for loss...