A5035559189- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Legume Nitrogen Fixing Symbiosis
- Crystallography and molecular interactions
- Supramolecular Chemistry and Complexes
- Physics and Engineering Research Articles
- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Proteins in Food Systems
- German Literature and Culture Studies
- Molecular Sensors and Ion Detection
- Phytase and its Applications
- Plant Virus Research Studies
- Livestock and Poultry Management
- Animal Vocal Communication and Behavior
- RNA and protein synthesis mechanisms
- Inorganic and Organometallic Chemistry
- Transgenic Plants and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- CRISPR and Genetic Engineering
- Hemoglobin structure and function
- Porphyrin and Phthalocyanine Chemistry
- Supramolecular Self-Assembly in Materials
- Agricultural pest management studies
- Chemical Synthesis and Analysis
Bayer (Germany)
2017-2024
TU Bergakademie Freiberg
1995-2011
Genetikum
1991-2007
Martin Luther University Halle-Wittenberg
2005-2006
Université Claude Bernard Lyon 1
2004
Centre National de la Recherche Scientifique
2004
Museo De Arte Contemporaneo
1992-1997
RELX Group (United Kingdom)
1997
University of Cape Town
1994
Oxford University Press (United Kingdom)
1992
The field of synthetic biology promises to revolutionize biotechnology through the design organisms with novel phenotypes useful for medicine, agriculture and industry. However, a limiting factor is ability current methods assemble complex DNA molecules encoding multiple genetic elements in various predefined arrangements. We present here hierarchical modular cloning system that allows creation at will high efficiency any eukaryotic multigene construct, starting from libraries defined...
The gram-negative plant-pathogenic bacterium Xanthomonas campestris pv. vesicatoria is the causative agent of bacterial spot disease in pepper and tomato plants, which leads to economically important yield losses. This pathosystem has become a well-established model for studying infection strategies. Here, we present whole-genome sequence pepper-pathogenic strain 85-10, comprises 5.17-Mb circular chromosome four plasmids. genome high G+C content (64.75%) signatures extensive plasticity....
Recent progress in the field of synthetic biology has led to creation cells containing genomes. Although these first organisms contained copies natural genomes, future work will be directed toward engineering with modified genomes and novel phenotypes. Much work, however, remains done able routinely engineer biological functions. As a tool that useful for such purpose, we have recently developed modular cloning system (MoClo) allows high throughput assembly multiple genetic elements. We...
Generation of customized DNA binding domains targeting unique sequences in complex genomes is crucial for many biotechnological applications. The recently described domain the transcription activator-like effectors (TALEs) from Xanthomonas consists a series repeats arranged tandem, each repeat nucleotide target sequence. We present here strategy engineering TALE proteins with novel specificities based on 17.5 repeat-containing AvrBs3 as scaffold. For 17 full repeats, four module types were...
Summary A library of synthetic promoters containing the binding site a single designer transcription activator‐like effector (dTALE) was constructed. The contain constant sequence, consisting an 18‐base long dTALE‐binding and TATA box, flanked by degenerate sequences 49 bases downstream 19 upstream. Forty‐three these were sequenced tested in transient assays Nicotiana benthamiana using GUS reporter gene. strength expression ranged from around 5% to almost 100% viral 35S promoter activity. We...
The plant pathogenic bacterium Xanthomonas campestris pv. vesicatoria expresses a type III secretion system that is necessary for both pathogenicity in susceptible hosts and the induction of hypersensitive response resistant plants. This specialized protein transport encoded by 23-kb hrp (hypersensitive pathogenicity) gene cluster. Here we show X. produces filamentous structures, Hrp pili, at cell surface under hrp-inducing conditions. Analysis purified pili immunoelectron microscopy...
The Gram-negative plant pathogenic bacterium Xanthomonas campestris pv. vesicatoria translocates effector proteins via a specialized type III secretion (TTS) system into the host cell cytosol. efficient of many depends on global TTS chaperone HpaB. Here, we identified novel export control protein, HpaC, which significantly contributes to bacterial pathogenicity. Deletion hpaC leads severe reduction in and putative translocon HrpF XopA. By contrast, pilus protein HrpE is not affected. We...