A5059070281- Plant Molecular Biology Research
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- Plant nutrient uptake and metabolism
- Polysaccharides and Plant Cell Walls
- Plant Stress Responses and Tolerance
- Microtubule and mitosis dynamics
- Plant-Microbe Interactions and Immunity
- Cellular transport and secretion
- Advanced Fluorescence Microscopy Techniques
- Legume Nitrogen Fixing Symbiosis
- Plant Parasitism and Resistance
- Transgenic Plants and Applications
- Seed Germination and Physiology
- Plant Pathogens and Fungal Diseases
- Plant Genetic and Mutation Studies
- Plant and animal studies
- Plant Gene Expression Analysis
- Fungal and yeast genetics research
- Chromosomal and Genetic Variations
- Plant and Biological Electrophysiology Studies
- Banana Cultivation and Research
- Heat shock proteins research
- Mitochondrial Function and Pathology
Palacký University Olomouc
2015-2024
Czech Academy of Sciences, Biology Centre
2011-2022
Natural (Czechia)
2016
Institute of Molecular and Translational Medicine
2016
Centre of the Region Haná for Biotechnical and Agricultural Research
2014
Institute of Plant Genetics and Biotechnology of the Slovak Academy of Sciences
2002-2013
Slovak Academy of Sciences
2002-2013
University of Bonn
1999-2011
Mississippi State University
2010
Norwich Research Park
2009
Eukaryotic cells typically deviate from spherical shapes due to complex interactions between elements of their cytoskeleton and the extracellular matrix (ECM). Communication ECM is one most characteristic features cellular mechanics allows respond
Abstract Brefeldin A (BFA) inhibits exocytosis but allows endocytosis, making it a valuable agent to identify molecules that recycle at cell peripheries. In plants, formation of large intracellular compartments in response BFA treatment is unique feature some, not all, cells. Here, we have analyzed assembly and distribution development- tissue-specific contexts growing maize (Zea mays) root apices. Surprisingly, these formed only meristematic cells the body. On other hand, were absent from...
Summary Myosins are a large superfamily of motor proteins which, in association with actin, involved intra‐ cellular motile processes. In addition to the conventional myosins muscle contractility, there is, animal cells, wide range unconventional implicated membrane‐associated processes, such as vesicle transport and membrane dynamics. plant however, very little is known about myosins. We have raised an antibody recombinant tail region Arabidopsis thaliana myosin 1 (a class VIII myosin) used...
Endocytosis is essential for the maintenance of protein and lipid compositions in plasma membrane acquisition materials from extracellular space. Clathrin-dependent -independent endocytic processes are well established yeast animals; however, pathways involved cargo internalization intracellular trafficking remain to be fully elucidated plants. Here, we used transgenic green fluorescent protein–flotillin1 (GFP - Flot1) Arabidopsis thaliana plants combination with confocal microscopy analysis...
The yeast open reading frames YOL027 and YPR125 their orthologs in various eukaryotes encode proteins with a single predicted trans-membrane domain ranging molecular mass from 45 to 85 kDa. Hemizygous deletion of human homolog LETM1 is likely contribute the Wolf-Hirschhorn syndrome phenotype. We show here that cells, these genes integral inner mitochondrial membrane. Deletion gene (yol027Δ mutation) results dysfunction. This mutant phenotype complemented by expression yeast, indicating...
Abstract A double homozygous recessive mutant in the Arabidopsis thaliana homologs of nucleus- and phragmoplast-localized kinase 2 (ANP2) 3 (ANP3) genes a mitogen-activated protein 4 (MPK4) gene exhibit deficiencies overall microtubule (MT) organization, which result abnormal cell growth patterns, such as branching root hairs swelling diffusely growing epidermal cells. Genetic, pharmacological, molecular, cytological, biochemical analyses show that major underlying mechanism for these...
The polarization of sterol-enriched lipid microdomains has been linked to morphogenesis and cell movement in diverse types. Recent biochemical evidence confirmed the presence plant cells; however, direct for a functional link between these growth is still lacking. Here, we reported involvement NADPH oxidase (NOX)-dependent reactive oxygen species (ROS) signaling Picea meyeri pollen tube growth. Staining with di-4-ANEPPDHQ or filipin revealed that were polarized growing tip tube. Sterol...
The dual-affinity nitrate transceptor NITRATE TRANSPORTER1.1 (NRT1.1) has two modes of transport and signaling, governed by Thr-101 (T101) phosphorylation. NRT1.1 regulates lateral root (LR) development modulating nitrate-dependent basipetal auxin export nitrate-mediated signal transduction. Here, using the Arabidopsis (Arabidopsis thaliana) NRT1.1T101D phosphomimetic NRT1.1T101A nonphosphorylatable mutants, we found that phosphorylation state plays a key role in function during LR...
Summary The role of YODA MITOGEN ACTIVATED PROTEIN KINASE 4 (MAPKKK4) upstream 6 (MPK6) was studied during post‐embryonic root development Arabidopsis thaliana . Loss‐ and gain‐of‐function mutants ( yda1 ΔNyda1 ) were characterized in terms patterning, endogenous auxin content global proteomes. We surveyed morphological cellular phenotypes suggesting possible involvement auxin. Endogenous indole‐3‐acetic acid (IAA) levels up‐regulated both mutants. Proteomic analysis revealed up‐regulation...
The carnivorous sundew plant (Drosera capensis) captures prey using sticky tentacles. We investigated the tentacle and trap reactions in response to electrical jasmonate signalling evoked by different stimuli reveal how sundews recognize digestible captured their traps. measured signals, phytohormone concentration, enzyme activities Chla fluorescence mechanical stimulation, wounding or insect feeding local systemic Seven new proteins digestive fluid were identified mass spectrometry....