A5050838520- Plant Molecular Biology Research
- Insect-Plant Interactions and Control
- Food composition and properties
- Plant Parasitism and Resistance
- Photosynthetic Processes and Mechanisms
- Plant and animal studies
- Plant nutrient uptake and metabolism
- Polysaccharides and Plant Cell Walls
- Plant-Microbe Interactions and Immunity
- Plant Gene Expression Analysis
- Plant Stress Responses and Tolerance
- Plant Reproductive Biology
- Genomics and Phylogenetic Studies
- Insect and Pesticide Research
- Plant biochemistry and biosynthesis
- Soil Carbon and Nitrogen Dynamics
- Plant pathogens and resistance mechanisms
- Probiotics and Fermented Foods
- Plant Micronutrient Interactions and Effects
- Leaf Properties and Growth Measurement
- Animal Virus Infections Studies
- Molecular Biology Techniques and Applications
- Plant Pathogenic Bacteria Studies
- Agriculture and Biological Studies
- Heavy metals in environment
Tsinghua University
2007-2025
Center for Life Sciences
2022-2025
Beijing Forestry University
2015-2024
University of North Carolina at Chapel Hill
2019-2021
Hunan Agricultural University
2011
Institute of Molecular and Cell Biology
2010
Zhejiang University
2010
Anthocyanins are important plant pigments that fulfil many physiological and ecological functions. Anthocyanin biosynthesis is controlled by numerous regulatory factors at the transcriptional level. Jasmonates (JAs) has been shown to induce anthocyanin accumulation in several species, however, molecular mechanism for JA-regulated remains unknown. In this study, genetic, molecular, approaches were used reveal basis of pigmentation Arabidopsis. It was found F-box protein COI1 required...
Abstract Leaf senescence, as the last stage of leaf development, is regulated by diverse developmental and environmental factors. Jasmonates (JAs) have been shown to induce senescence in several plant species; however, molecular mechanism for JA-induced remains unknown. In this study, proteomic, genetic, physiological approaches were used reveal basis Arabidopsis (Arabidopsis thaliana). We identified 35 coronatine-insensitive 1 (COI1)-dependent JA-regulated proteins using two-dimensional...
The dual-affinity nitrate transceptor NITRATE TRANSPORTER1.1 (NRT1.1) has two modes of transport and signaling, governed by Thr-101 (T101) phosphorylation. NRT1.1 regulates lateral root (LR) development modulating nitrate-dependent basipetal auxin export nitrate-mediated signal transduction. Here, using the Arabidopsis (Arabidopsis thaliana) NRT1.1T101D phosphomimetic NRT1.1T101A nonphosphorylatable mutants, we found that phosphorylation state plays a key role in function during LR...
NITRATE TRANSPORTER 1.1 (NRT1.1) functions as a dual affinity nitrate transceptor regulated by phosphorylation at threonine residue 101 (T101). Previous studies have suggested that NRT1.1 is involved in stomatal opening and contributes to drought susceptibility. However, the precise mechanism of how status affects movement tolerance remains unclear. In this study, we observed seedlings expressing phosphorylated form (NRT1.1T101D, T101D) exhibited increased compared dephosphorylated...
The chloroplast, as the photosynthetic organelle of plants, plays a crucial role in plant development. Extensive studies have been conducted on chloroplast development; however, related regulatory mechanism still remains elusive. Here, we characterized mutant with defective chloroplasts Arabidopsis, termed pigment-defective mutant3 (pdm3), which exhibits distinct albino phenotype leaves, eventually leading to pdm3 seedling lethality under autotrophic growth conditions. Electron microscopy...
Cunninghamia lanceolata (Chinese fir) is a fast-growing and commercially important conifer of the Cupressaceae family. Due to unavailability complete genome sequences relatively poor genetic background information Chinese fir, it necessary identify analyze expression levels suitable housekeeping genes (HKGs) as internal reference for precise analysis. Based on results database analysis transcriptome sequencing, we have chosen five candidate HKGs (Actin, GAPDH, EF1a, 18S rRNA, UBQ) with...
Segregated pathways enable AtRGS1 endocytosis to transduce signal-specific responses in Arabidopsis .
Abstract Jasmonates (JAs), which include jasmonic acid and its cyclopentane derivatives are synthesized from the octadecanoid pathway widely distributed throughout plant kingdom. JAs modulate expression of numerous genes mediate responses to stress, wounding, insect attack, pathogen infection, UV damage. They also affect a variety processes in many developmental processes. The JA signal involves two important events: biosynthesis transduction signal. Several Arabidopsis mutants jasmonate...
Abstract Jasmonates (JAs) are a new class of plant hormone that regulate expression diverse genes to mediate various responses. The Arabidopsis F‐box protein COI1 is required for defense and male fertility in JA signal pathway. To further investigate the regulatory role fertility, we compared proteomics profiles wild type (WT) flowers with coi1‐1 mutant male‐sterile using two‐dimensional difference gel electrophoresis coupled matrix‐assisted laser desoption/ionization‐time‐of‐flight mass...
Abstract Enhanced autoimmunity often leads to impaired plant growth and development, the coordination of immunity in Populus remains elusive. In this study, we have identified transcription factors PagWRKY33a/b as key regulators immune response maintenance Populus. The disruption causes issues while conferring resistance anthracnose caused by Colletotrichum gloeosporioides. binds promoters N requirement gene 1.1 (NRG1.1) Gibberellic Acid-Stimulated Arabidopsis (GASA14) during infection,...
Arabidopsis heterotrimeric G proteins regulate diverse plant growth and defense processes by coupling to 7TM AtRGS1 proteins. Although protein mutants display alterations in response multiple hormones, the underlying mechanism which participate regulation of hormone responses remains elusive. Here, we show that genetic disruption Gα Gβ subunits results reduced sensitivity JA treatment. Furthermore, using confocal microscopy, VA-TIRFM, FRET-FLIM, provide evidence stimulation induces...
Abstract Background The plasma membrane (PM) proteins function in a highly dynamic state, including protein trafficking and homeostasis, to regulate various biological processes. dwell time colocalization of PM are considered be two important features determining endocytosis interactions, respectively. Dwell-time detected using traditional fluorescence microscope techniques often misestimated due bulk measurement. In particular, analyzing these at the single-molecule level with...
Jasmonates (JAs) induce leaf senescence in many plant species. The Arabidopsis F-box protein coronatine insensitive 1 (COI1) is required for various JA-regulated responses including fertility, defense and senescence. However, the molecular basis COI1-dependent JA-induced remains unknown. In our Plant Physiology paper, we identified a JA-repressed protein, Rubisco activase (RCA) Arabidopsis. Further genetic physiological analyses showed that JA repression of RCA correlated with senescence,...
Lead is a heavy metal known to be toxic both animals and plants. Nitric oxide (NO) was reported participate in plant responses different stresses. In this study, we analyzed the function of exogenous endogenous NO Pb-induced toxicity tobacco BY-2 cells, focusing on role generation reactive oxygen species (ROS) as well Pb2+ Ca2+ fluxes using non-invasive micro-test technology (NMT). Pb treatment induced cell death rapid ROS generation, while burst occurred earlier than accumulation. The...
The increasing number of novel approaches for large-scale, multi-dimensional imaging cells has created an unprecedented opportunity to analyze plant morphogenesis. However, complex image processing, including identifying specific and quantitating parameters, high running cost some analysis softwares remains challenging. Therefore, it is essential develop efficient method multicellularity in raw micrographs plants.Here, we developed a high-efficiency procedure characterize, segment, quantify...