A5074458530- Cellular transport and secretion
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Legume Nitrogen Fixing Symbiosis
- Lipid Membrane Structure and Behavior
- Plant Molecular Biology Research
- Endoplasmic Reticulum Stress and Disease
- Plant nutrient uptake and metabolism
- Polysaccharides and Plant Cell Walls
- Plant tissue culture and regeneration
- Plant and Biological Electrophysiology Studies
- Calcium signaling and nucleotide metabolism
- Transgenic Plants and Applications
- Plant Parasitism and Resistance
- Photoreceptor and optogenetics research
- Pancreatic function and diabetes
- Crystallization and Solubility Studies
- Archaeology and Natural History
- X-ray Diffraction in Crystallography
- Autophagy in Disease and Therapy
- ATP Synthase and ATPases Research
- Glycosylation and Glycoproteins Research
- Toxin Mechanisms and Immunotoxins
- Plant Stress Responses and Tolerance
- Plant Virus Research Studies
Heidelberg University
2014-2024
University of Glasgow
2023
University of California, Santa Cruz
2023
University of Idaho
2023
Stephen F. Austin State University
2015
Heidelberg University
2006-2013
Sandia National Laboratories California
2012
Pohang University of Science and Technology
2012
BOKU University
2011
Chinese University of Hong Kong
2007-2008
Abstract Plants constantly adjust their repertoire of plasma membrane proteins that mediates transduction environmental and developmental signals as well transport ions, nutrients, hormones. The importance regulated secretory endocytic trafficking is becoming increasingly clear; however, our knowledge the compartments molecular machinery involved still fragmentary. We used immunogold electron microscopy confocal laser scanning to trace route cargo molecules, including BRASSINOSTEROID...
Little is known about the dynamics and molecular components of plant prevacuolar compartments (PVCs). We have demonstrated recently that vacuolar sorting receptor (VSR) proteins are concentrated on PVCs. In this study, we generated transgenic Nicotiana tabacum (tobacco) BY-2 cell lines expressing two yellow fluorescent protein (YFP)-fusion reporters mark PVC Golgi organelles. Both exhibited typical punctate YFP signals corresponding to distinct organelles because reporter colocalized with...
Abstract The exocyst protein complex mediates vesicle fusion with the plasma membrane. By expressing an (X)FP-tagged Arabidopsis thaliana homolog of Exo70 in suspension-cultured and tobacco (Nicotiana tabacum) BY-2 cells, using antibodies specific for Exo70, we detected a compartment, which term EXPO (for positive organelles). Standard markers Golgi apparatus, trans-Golgi network/early endosome, multivesicular body/late endosome plants do not colocalize EXPO. Inhibitors secretory endocytic...
Abstract The plant trans-Golgi network/early endosome (TGN/EE) is a major hub for secretory and endocytic trafficking with complex molecular mechanisms controlling sorting transport of cargo. Vacuolar from the TGN/EE to multivesicular bodies/late endosomes (MVBs/LEs) assumed occur via clathrin-coated vesicles, although direct proof their participation missing. Here, we present evidence that post-TGN toward lytic vacuoles occurs independently clathrin MVBs/LEs are derived through maturation....
Brefeldin A (BFA) causes a block in the secretory system of eukaryotic cells by inhibiting vesicle formation at Golgi apparatus. Although this toxin has been used many studies, its effects on plant are still shrouded controversy. We have reinvestigated early responses to BFA with novel tools, namely, tobacco Bright Yellow 2 (BY-2) suspension-cultured expressing an vivo green fluorescent protein–Golgi marker, electron microscopy high-pressure frozen/freeze-substituted cells, and antisera...
Abstract We recently identified multivesicular bodies (MVBs) as prevacuolar compartments (PVCs) in the secretory and endocytic pathways to lytic vacuole tobacco (Nicotiana tabacum) BY-2 cells. Secretory carrier membrane proteins (SCAMPs) are post-Golgi, integral mediating endocytosis animal To define pathway plants, we cloned rice (Oryza sativa) homolog of SCAMP1 generated transgenic cells expressing yellow fluorescent protein (YFP)–SCAMP1 or SCAMP1-YFP fusions. Confocal immunofluorescence...
Abstract Brefeldin A (BFA) inhibits exocytosis but allows endocytosis, making it a valuable agent to identify molecules that recycle at cell peripheries. In plants, formation of large intracellular compartments in response BFA treatment is unique feature some, not all, cells. Here, we have analyzed assembly and distribution development- tissue-specific contexts growing maize (Zea mays) root apices. Surprisingly, these formed only meristematic cells the body. On other hand, were absent from...
Many aspects of plant development, including patterning and tropisms, are largely dependent on the asymmetric distribution signaling molecule auxin. Auxin transport inhibitors (ATIs), which interfere with directional auxin transport, have been essential tools in formulating this concept. However, despite use ATIs research for many decades, mechanism ATI action has remained elusive. Using real-time live-cell microscopy, we show here that prominent such as 2,3,5-triiodobenzoic acid (TIBA)...
Grapevine fanleaf virus (GFLV) is one of a large class plant viruses whose cell-to-cell transport involves the passage virions through tubules composed virus-encoded movement protein (MP). The are embedded within modified plasmodesmata, but mechanism targeting MP to these sites unknown. To study intracellular GFLV trafficking, green fluorescent protein-MP fusion (GFP:MP) was expressed in transgenic tobacco BY-2 suspension cells under control an inducible promoter. We show that GFP:MP...
Coat protein (COP)–coated vesicles have been shown to mediate transport through early steps of the secretory pathway in yeast and mammalian cells. Here, we attempt elucidate their role vesicular trafficking plant cells, using a combined biochemical ultrastructural approach. Immunogold labeling cryosections revealed that COPI proteins are localized microvesicles surrounding or budding from Golgi apparatus. COPI-coated buds primarily reside on cis-face stack. In addition, Arf1p show...
COPII-coated vesicles, first identified in yeast and later characterized mammalian cells, mediate protein export from the endoplasmic reticulum (ER) to Golgi apparatus within secretory pathway. In these organisms, mechanism of vesicle formation is well understood, but process soluble cargo sorting has yet be resolved. plants, functional complements COPII-dependent traffic machinery were almost a decade ago, selectivity ER been subject considerable debate. To study we have developed an vivo...
Abstract Receptors for acid hydrolases destined the lytic compartment in yeast and mammalian cells are retrieved from intermediate, endosomal organelles with help of a pentameric protein complex called retromer. We cloned Arabidopsis thaliana homologs three proteins (Vps35, Vps29, Vps26) constituting larger subunit retromer prepared antisera against them. With these antibodies, we demonstrated presence retromer-like salt extracts microsomes. This is associated membranes that coequilibrate...
Developing pea cotyledons contain functionally different vacuoles, a protein storage vacuole and lytic vacuole. Lumenal as well membrane proteins of the exit Golgi apparatus in dense vesicles rather than clathrin-coated (CCVs). Although sorting receptor for vacuolar hydrolases BP-80 is present CCVs, it not detectable vesicles. To localize these events Golgi, we have compared distribution α-TIP, vacuole, with across stack. Analysis immunogold labeling from cryosections high pressure frozen...
Abstract Despite the ubiquitous presence of COPI, COPII, and clathrin vesicle budding machineries in all eukaryotes, organization secretory pathway plants differs significantly from that yeast mammalian cells. Mobile Golgi stacks lack both transitional endoplasmic reticulum (ER) a distinct ER-to-Golgi intermediate compartment are most prominent distinguishing morphological features early plants. Although formation COPI vesicles at periphery cisternae has been demonstrated plants, exit ER...