A5074970290- Ferroelectric and Piezoelectric Materials
- Microwave Dielectric Ceramics Synthesis
- Cellular transport and secretion
- Dielectric materials and actuators
- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Hearing, Cochlea, Tinnitus, Genetics
- RNA Research and Splicing
- Particle accelerators and beam dynamics
- CRISPR and Genetic Engineering
- Microplastics and Plastic Pollution
- Silkworms and Sericulture Research
- RNA regulation and disease
- Ear Surgery and Otitis Media
- Silk-based biomaterials and applications
- Neutrino Physics Research
- Plant Reproductive Biology
- Hearing Loss and Rehabilitation
- Multiferroics and related materials
- RNA and protein synthesis mechanisms
- Brucella: diagnosis, epidemiology, treatment
- Genetics and Neurodevelopmental Disorders
- Axon Guidance and Neuronal Signaling
- Edible Oils Quality and Analysis
- Infant Development and Preterm Care
Hubei University of Chinese Medicine
2024-2025
Nanjing University
2023-2024
Collaborative Innovation Center of Advanced Microstructures
2023-2024
Ningxia Medical University
2024
Shandong University
2023
University of Otago
2020
Northwest University
2011-2019
State Key Laboratory For Conservation and Utilization of Subtropical Agro-Bioresources
2018
Guangxi University
2018
China Rehabilitation Research Center
2011-2017
Little is known about the dynamics and molecular components of plant prevacuolar compartments (PVCs). We have demonstrated recently that vacuolar sorting receptor (VSR) proteins are concentrated on PVCs. In this study, we generated transgenic Nicotiana tabacum (tobacco) BY-2 cell lines expressing two yellow fluorescent protein (YFP)-fusion reporters mark PVC Golgi organelles. Both exhibited typical punctate YFP signals corresponding to distinct organelles because reporter colocalized with...
In this study, male Sprague-Dawley (SD) rats were exposed to bisphenol S (BPS) at environmentally relevant concentrations investigate its reproductive toxicity and evaluate effects on the gut-blood-testicular axis. After 28 days of exposure BPS (0.05 20 mg/kg), results showed a reduction in weight gain induction rats, including decreased sperm parameters, lower viability, increased abnormal density mortality. These observations made by counting with hemocytometer under optical microscope....
Rab proteins are members of the Ras superfamily small GTP binding and play important roles in various intracellular trafficking steps. We investigated role Rha1, an Arabidopsis Rab5 homolog, protoplasts. In presence a dominant-negative mutant soluble vacuolar cargo such as sporamin:green fluorescent protein (Spo:GFP) aleurain like protein:GFP not delivered to central vacuole; instead, they accumulate diffuse or punctate staining pattern within cell. Spo:GFP at stains observed...
A microscopy-based screen of a large collection maize Mutator (Mu) transposon lines identified the supernumerary aleurone layers 1-1 (sal1-1) mutant line carrying up to seven cells in defective kernel endosperm compared with only single layer wild-type grains. Normal, well filled that is homozygous for sal1-1 allele contains two three cells. Cloning sal1 gene was accomplished by using Mu tagging, and identity cloned confirmed isolating an independent sal1-2 reverse genetics. Homozygous has...
Development of the aleurone layer maize grains requires activity <i>Defective kernel 1 (Dek1</i>) gene, encoding a predicted 240-kDa membrane-anchored protein with C terminus similar to animal calpain domain II&III. Three-dimensional modeling shows that DEK1 II contains conserved catalytic triad and II&III has structure m-calpain. Recombinant exhibits in caseinolytic assay absence calcium, although is enhanced by calcium. This sharp contrast calpains, which require Ca<sup>2</sup><sup>+</sup>...
Abstract A novel functional absorbent, polyvinyltetrazole fiber (PVT), was made from polyacrylonitrile through [3+2] azide‐nitrile cycloaddition reaction of cross‐linked with sodium azide. The obtained PVT showed strong adsorption ability to the investigated heavy‐metal ions, Cu(II), Cd(II), Ni(II), and Zn(II), maximum capacities 323, 278, 200, 175 mg g −1 , respectively. process described as an ion exchange mechanism. isotherms were better fitted for Langmuir model, while kinetics by...
Abstract Sexual dimorphism is a widespread phenomenon in many higher animals. The genes and gene networks that underlie sex differences are poorly understood. Using microarray data we analyzed sex-related the global expression profiles of silkworm from larval to pupal stages. Sex-biased could be divided into three clusters. Cluster 1 contained 932 showed female-biased trend at first male-biased afterward. 2 included 283 genes. 3 was comprised 497 were expressed during late stage. found...
Raw camel milk is generally contaminated with varied microbiota, including antibiotic–resistant bacteria (ARB), that can act as a potential pathway for the spread of antibiotic resistance genes (ARGs). In this study, high–throughput quantitative PCR and 16S rRNA gene–based Illumine sequencing data were used to establish comprehensive understanding resistome its relationship bacterial community in Bactrian from Xinjiang. A total 136 ARGs up 1.33 × 108 ARG copies per gram identified, which...
Abstract To develop an easy and robust method for creating genetically stable easily detectable Arabidopsis mutants, we adopted the polycistronic tRNA–gRNA CRISPR/Cas9 (PTG/Cas9) system, a multiplex gene-editing tool in rice, with PTOX as reporter gene. The PTG/Cas9 system has great potential generating large deletions by PCR, which greatly simplifies laborious work of mutant screening. We constructed –PTG/Cas9 five gRNAs introduced it into . At T1 generation, 24.4% transgenic plants were...
Two-component regulatory system (TCS), a major type of cellular signal transduction system, is widely used by bacteria to adapt different conditions and colonize certain ecological niches in response environmental stimuli. TCSs are distinct functional diversity, genetic species specificity (pathovar specificity, even strain specificity) across bacterial groups. Although have been demonstrated be crucial the virulence Xanthomonas, only few researches reported about studies Xanthomonas oryzae...
Introduction.Pseudomonas syringae pv. actinidiae (Psa) has emerged as a major bacterial pathogen of kiwifruit cultivation throughout the world.Aim. We aim to introduce CRISPR-Cas9 system, commonly used genome editing tool, into Psa. The protocols may also be useful in other Pseudomonas species.Methodology. Using standard molecular biology techniques, we modified plasmid pCas9, which carries sequences from Streptococcus pyogenes, for use final plasmid, pJH1, was produced series steps and is...
The Bombyx mori sex determination gene Bmdsx is alternatively spliced in the male and female to produce male- female-specific proteins. In an effort better understand mechanism of alternative splicing regulation Bmdsx, we conducted a gel-shift assay followed by LC-MS/MS analysis identify putative proteins bound cis-element CE1+6 exon 4 Bmdsx. A protein named as BmHrp28 which homologous Drosophila Hrp48, member hnRNPA/B family, was identified expressed Escherichia coli for testing RNA-protein...
Introduction: Usher syndrome (USH) is the most common cause of hereditary deaf-blindness. Three clinical subtypes have been classified. USH type I severe subtype characterized by congenital severe-to-profound hearing loss, retinitis pigmentosa and vestibular dysfunction. Methods: One family was analyzed analysis included combination a custom capture/next-generation sequencing panel 180 known deafness gene, Sanger bioinformatics approaches. Results: Compound heterozygous mutations in MYO7A...
The electrocaloric effect (ECE) is a novel technology that offers high efficiency and environmental friendliness, making it suitable for solid-state refrigeration applications. Among the extensively studied ECE materials, lead scandium tantalate (PST) stands out its excellent performance. However, applications are restricted by narrow working temperature range. To overcome this limitation, we explore enhancement of through zirconium ion doping. We synthesized PbSc0.5–0.5xTa0.5–0.5xZrxO3...
Abstract Heaping is an unavoidable process before olive milling, and its duration significantly affects the quality. However, there limited research on quality changes of fruits a short-time scale. To gain better understanding molecular mechanisms underlying postharvest deterioration olives, this study piled olives at room temperature extracted oil 0, 8, 24, 48 72 h to analyze parameters. Gas/Liquid Chromatography-Mass Spectrometry (GC/LC–MS) techniques were employed investigate variations...