A5100325315- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- China's Socioeconomic Reforms and Governance
- Hippo pathway signaling and YAP/TAZ
- Immunotherapy and Immune Responses
- Virus-based gene therapy research
- Nanoparticle-Based Drug Delivery
- Protein Tyrosine Phosphatases
- Nanoplatforms for cancer theranostics
- Cancer-related molecular mechanisms research
- TGF-β signaling in diseases
- Glioma Diagnosis and Treatment
- Cancer Cells and Metastasis
- RNA Research and Splicing
- RNA modifications and cancer
- Ethnobotanical and Medicinal Plants Studies
- China's Ethnic Minorities and Relations
- Cellular Mechanics and Interactions
- Mechanisms of cancer metastasis
- Ubiquitin and proteasome pathways
- Hong Kong and Taiwan Politics
- Monoclonal and Polyclonal Antibodies Research
- Microtubule and mitosis dynamics
- Higher Education and Teaching Methods
- Judicial and Constitutional Studies
Pudong New Area People's Hospital
2022-2025
Wuhan University
2007-2024
Tianjin University of Science and Technology
2005-2024
People's Hospital of Cangzhou
2024
Anhui Medical University
2023-2024
Guangzhou University
2024
Chinese Academy of Sciences
2009-2023
Kunming Institute of Botany
2020-2023
Fujian Normal University
2013-2022
China Agricultural University
2011-2021
Trastuzumab and lapatinib provide clinical benefit to women with human epidermal growth factor receptor 2 (HER)-positive breast cancer. However, not all patients whose tumors contain the HER2 alteration respond. Consequently, there is an urgent need identify new predictive factors for these agents. The aim of this study was investigate role tyrosine kinase signaling phosphoinositide 3-kinase (PI3K)/AKT pathway activation in conferring resistance trastuzumab lapatinib. To address question, we...
Triple negative breast cancer (TNBC), which constitutes 10%-20% of all cancers, is associated with aggressive progression, a high rate metastasis, and poor prognosis. The treatment patients TNBC remains great clinical challenge. Preclinical reports support the combination immunotherapy vaccines immune checkpoint blockades in non-immunogenic tumors. In this study, we constructed nanoparticles (NPs) to deliver an mRNA vaccine encoding tumor antigen MUC1 dendritic cells (DCs) lymph nodes...
Achievement of potent immunoresponses against self/tumor antigens and effective therapeutic outcome advanced tumors remain major challenges in cancer immunotherapy. The specificity efficiency two nanoparticle-based delivery systems, lipid-calcium-phosphate (LCP) nanoparticle (NP) liposome-protamine-hyaluronic acid (LPH) NP, provide us an opportunity to address both challenges. A mannose-modified LCP NP delivered tumor antigen (Trp 2 peptide) adjuvant (CpG oligonucleotide) the dendritic cells...
Abstract Over the past two decades, Chinese domestic security apparatus has expanded dramatically. “Stability maintenance” operations have become a top priority for local authorities. We argue that this trend goes back to early 1990s, when central Party authorities adopted new governance models differed dramatically from those of 1980s. They increased bureaucratic rank public chiefs within apparatus, reach political-legal into broader range issues, and altered cadre evaluation standards...
Background and Objective. Exosomes secreted from mesenchymal stem cells (MSC) have demonstrated cardioprotective effects. This study examined the role of exosomes derived MSC overexpressing CXCR4 for recovery cardiac functions after myocardial infarction (MI). Methods. In vitro , transduced with lentiviral (Exo CR4 ) encoding a silencing sequence or null vector were isolated characterized by transmission electron microscopy dynamic light scattering. Gene expression was then analyzed qPCR...
We reported a preclinical cancer vaccine that simultaneously introduced an mRNA antigen and immune checkpoint blocking siRNA into the antigen-presenting cells. This was achieved by formulating both nucleic acid-based immunotherapeutics lipid-coated calcium phosphate (LCP) nanoparticle (NP) as carrier to address delivery challenge. The PEGylated lipid NPs were functionalized with mannose targeting ligand facilitate preferential uptake dendritic cells (DCs) in lymph nodes after subcutaneous...
CD47 is a "self marker" that usually overexpressed on the surface of cancer cells to enable them escape immunosurveillance. Recognition by its receptor, signal regulatory protein α (SIRPα), which expressed in macrophages, inhibits phagocytic destruction macrophages. In this study, we have first shown clinical isolates human melanoma significantly upregulate CD47, possibly as mechanism defend themselves against We then exploited RNA interference (RNAi) technology test hypothesis knocking down...
Failure of clinical trials nonviral vector-mediated gene therapy arises primarily from either an insufficient transgene expression level or immunostimulation concerns caused by the genetic information carrier (e.g., bacteria-generated, double-stranded DNA (dsDNA)). Neither these issues could be addressed through engineering-sophisticated delivery vehicles. Therefore, we propose a systemic chemically modified messenger RNA (mRNA) as alternative to plasmid (pDNA) in cancer therapy. Modified...
The tumor microenvironment plays an important role in the tumor's progression and metastasis. Therefore, successful alteration of this delicate setting against favor can open a window for therapeutic efficacy. We have developed modality to bring about treatment-induced alterations by employing synergistic effects between two drugs. Co-delivery rapamycin (RAPA), mTOR inhibitor that may offer notable therapy through antiangiogenic activity, alongside cisplatin foster significant potency as...
Abstract Metformin, a widely implemented anti-diabetic drug, exhibits potent anticancer efficacies. Herein polymeric construction of PolyMetformin (PolyMet) is successfully synthesized through conjugation linear polyethylenimine (PEI) with dicyandiamide. The delocalization cationic charges in the biguanide groups PolyMet reduces toxicity PEI both vitro and vivo . Furthermore, polycationic properties permits capture siRNA into core-membrane structured lipid-polycation-hyaluronic acid (LPH)...
Previously, we have reported a lipid-based Trp2 peptide vaccine for immunotherapy against melanoma. The suppressive immune microenvironment in the tumor is major hurdle an effective therapy. We hypothesized that curcumin (CUR) would remodel to improve activity. Curcumin–polyethylene glycol conjugate (CUR–PEG), amphiphilic CUR-based micelle, was delivered intravenously (i.v.) tumor. Indeed, B16F10 tumor–bearing mice, combination of CUR–PEG and treatment resulted synergistic antitumor effect...
Cerebral ischemia (CI) results from inadequate blood flow to the brain. The difficulty of delivering therapeutic molecules lesions resulting CI hinders effective treatment this disease. inflammatory response following offers a unique opportunity for drug delivery ischemic brain and targeted cells because recruitment leukocytes stroke core penumbra. In present study, neutrophils monocytes were explored as cell carriers after selectively carrying cRGD liposomes, which effectively transmigrated...
Non-small cell lung cancer (NSCLC) is the leading cause of cancer-related death. To explore potential small interfering RNA (siRNA) therapy for NSCLC, we have developed anisamide-targeted LCP to efficiently deliver siRNA into cytoplasm sigma receptor-expressing NSCLC cells. Targeted demonstrated a 9-fold higher delivery efficiency compared nontargeted in A549 cells vitro. simultaneously target multiple oncogenic mechanisms, coformulated three sequences targeting HDM2, c-myc and VEGF...
MicroRNAs have been appreciated in various cellular functions, including the regulation of angiogenesis. Mesenchymal-stem-cells (MSCs) transplanted to MI heart improve cardiac function through paracrine-mediated However, whether microRNAs regulate MSC induced angiogenesis remains be clarified. Using microRNA microarray analysis, we identified a expression profile hypoxia-treated MSCs and observed that among all dysregulated microRNAs, microRNA-377 was decreased most significantly. We also...
Multidrug resistance (MDR) is a major barrier to the chemotherapy treatment of many cancers. However, some nonionic surfactants, for example, Brij, have been shown restore sensitivity MDR cells such drugs. The aim this study was explore reversal effect Brij on tumor and elucidate its potential mechanism. Our data indicate that structure surfactants plays an important role in overcoming cancer, is, modified hydrophilic-lipophilic balance (MHLB, ratio number (n) hydrophilic repeating units...
Abstract This article presents a large-scale, systematic study of politically connected firms in China. It was conducted by compiling database all the publicly traded China 1993, 2002 and 2012 that codes biographies hundreds thousands board members. I find there has been significant increase percentage are with national government last 20 years. casts doubt on popular argument businesses have primarily relied “local protectionism.” interpret this as result firms' need to connect powerful...