A5049796989- Plant Molecular Biology Research
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Light effects on plants
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Plant Gene Expression Analysis
- Plant tissue culture and regeneration
- Advanced Radiotherapy Techniques
- Polysaccharides and Plant Cell Walls
- Plant Virus Research Studies
- Plant Physiology and Cultivation Studies
- Flowering Plant Growth and Cultivation
- Plant Genetic and Mutation Studies
- Plant responses to elevated CO2
- Plant and animal studies
- Plant Growth Enhancement Techniques
- Glioma Diagnosis and Treatment
- Chromosomal and Genetic Variations
- Digital Radiography and Breast Imaging
- Radiation Therapy and Dosimetry
- Lung Cancer Diagnosis and Treatment
- Brain Metastases and Treatment
- Radiation Dose and Imaging
- Heat shock proteins research
Ajou University
2024-2025
Kyungpook National University
2019-2023
Academia Sinica
2015-2021
Rural Development Administration
2021
Southern Taiwan Science Park
2015-2021
UPMC Hillman Cancer Center
2016-2020
Max Planck Institute for Plant Breeding Research
2006-2020
National Cheng Kung University
2015-2018
Agricultural Biotechnology Research Center, Academia Sinica
2014-2017
University of Arizona
2009-2011
In plants, seasonal changes in day length are perceived leaves, which initiate long-distance signaling that induces flowering at the shoot apex. The identity of signal has yet to be determined. Arabidopsis, activation FLOWERING LOCUS T (FT) transcription leaf vascular tissue (phloem) flowering. We found FT messenger RNA is required only transiently leaf. addition, fusion proteins expressed specifically phloem cells move apex and long distances between grafted plants. Finally, we provide...
Summary We have produced 22 090 primary transgenic rice plants that carry a T‐DNA insertion, which has resulted in 18 358 fertile lines. Genomic DNA gel‐blot and PCR analyses shown approximately 65% of the population contains more than one copy inserted T‐DNA. Hygromycin resistance tests revealed contain an average 1.4 loci inserts. Therefore, it can be estimated 25 700 taggings been generated. The binary vector used insertion contained promoterless β‐glucuronidase (GUS) reporter gene with...
Rice contains several MADS box genes. It has been demonstrated previously that one of these genes, OsMADS1 (for Oryza sativa gene1), is expressed preferentially in flowers and causes early flowering when ectopically tobacco plants. In this study, we ectopic expression rice also results flowering. To further investigate the role during flower development, generated transgenic plants expressing altered genes contain missense mutations domain. There was no visible alteration vegetative stage....
The four-member SPA protein family of Arabidopsis acts in concert with the E3 ubiquitin ligase COP1 to suppress photomorphogenesis dark-grown seedlings. Here, we demonstrate that proteins are, moreover, essential for photoperiodic flowering. Mutations SPA1 cause phyA-independent early flowering under short day (SD) but not long (LD) conditions, and this phenotype is enhanced by additional loss SPA3 SPA4 function. These spa1 spa3 spa4 triple mutants flower at same time LD SD, indicating gene...
Abstract We recently identified multivesicular bodies (MVBs) as prevacuolar compartments (PVCs) in the secretory and endocytic pathways to lytic vacuole tobacco (Nicotiana tabacum) BY-2 cells. Secretory carrier membrane proteins (SCAMPs) are post-Golgi, integral mediating endocytosis animal To define pathway plants, we cloned rice (Oryza sativa) homolog of SCAMP1 generated transgenic cells expressing yellow fluorescent protein (YFP)–SCAMP1 or SCAMP1-YFP fusions. Confocal immunofluorescence...
Summary Flowering is controlled by a network of pathways that converge to regulate small number floral integrator genes. We studied the interactions in Arabidopsis between three these integrators, FLOWERING LOCUS T ( FT ), TWIN SISTER OF TSF ) and SUPPRESSOR OVEREXPRESSION CONSTANS 1 SOC1 as well their repression MADS box transcription factor SHORT VEGETATIVE PHASE (SVP). mobile signal transmitted from leaf meristem initiate flowering. Using mRNA null alleles, we show closely related are not...
Article7 March 2017Open Access Transparent process PSEUDO RESPONSE REGULATORs stabilize CONSTANS protein to promote flowering in response day length Ryosuke Hayama Department of Plant Developmental Biology, Max Planck Institute for Breeding Research, Cologne, Germany Search more papers by this author Liron Sarid-Krebs René Richter Virginia Fernández Seonghoe Jang George Coupland Corresponding Author [email protected] orcid.org/0000-0001-6988-4172 Information Hayama1,2, Sarid-Krebs1,...
Rice atypical HLH protein Oryza sativa BRASSINOSTEROID UPREGULATED 1-LIKE1 (OsBUL1) is preferentially expressed in the lamina joint where it controls cell elongation and positively affects leaf angles. OsBUL1 knockout mutant (osbul1) transgenic rice for double-stranded RNA interference (dsRNAi) of produced erect leaves with smaller grains, whereas overexpressors an activation tagging line exhibited increased inclination grain size. Moreover, expression was induced by brassinolide (BL) osbul1...
Abstract Background Floral transition initiates reproductive development of plants and occurs in response to environmental endogenous signals. In Arabidopsis thaliana , this process is accelerated by several cues, including exposure long days. The photoperiod-dependent promotion flowering involves the transcriptional induction FLOWERING LOCUS T ( FT ) phloem leaf. encodes a mobile protein that transported from leaves shoot apical meristem, where it forms part regulatory complex induces...
Hepatocellular carcinoma (HCC) is characterized by high recurrence and mortality, necessitating the identification of reliable biomarkers. In this study, we aimed to identify predictive gene signatures for HCC evaluate efficiency GULP PTB domain-containing engulfment adaptor 1 (GULP1) as a diagnostic marker therapeutic target HCC. We analyzed genomic datasets from The Cancer Genome Atlas Gene Expression Omnibus databases via least absolute shrinkage selection operator Cox regression 10-fold...
Flowering of many plant species depends on interactions between basic leucine zipper (bZIP) transcription factors and systemically transported florigen proteins. Members the genus Arabidopsis contain two these bZIPs, FD FDP, which we show have largely complementary expression patterns in shoot apices before during flowering. CRISPR-Cas9-induced null mutants for FDP flower slightly earlier than wild-type, whereas fd are late Identical G-box sequences enriched at binding sites, but only binds...
We have generated 47 DNA pools and 235 subpools from 21,049 T-DNA insertion lines of rice. 500–1,000 were adequate for screening a within 2-kb region. To examine the efficacy pools, we selected MADS-box genes, which play an important role in controlling various aspects plant development. A total 34 MIKC-type genes now been identified rice sequence databases. Our PCR insertions 12 resulted identification five four different genes. These will be valuable when isolating insertional mutants The...
Seasonal flowering involves responses to changes in day length. In Arabidopsis thaliana, the CONSTANS (CO) transcription factor promotes long days of spring and summer. Late short is due instability CO, which efficiently ubiquitinated dark by CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) E3 ligase complex. Here we show that CO also phosphorylated. Phosphorylated unphosphorylated forms are detected throughout diurnal cycle but their ratio varies, with relative abundance phosphorylated form being...
Plants contain a variety of the MADS box genes that encode regulatory proteins and play important roles in both formation flower meristem determination floral organ identity. We have characterized two flower-specific cDNAs from rice, designated OsMADS7 OsMADS8. The displayed structure typical plant gene, which consists domain, I region, K C-terminal region. These were classified as members AGL2 gene family based on sequence homology. 8 most homologous to OM1 FBP2, respectively. transcripts...
We show that the key flowering regulators encoded by Phalaenopsis aphrodite FLOWERING LOCUS T1 (PaFT1) and PaFD share high sequence homologies to these from long-day Arabidopsis short-day rice. Interestingly, PaFT1 is specifically up-regulated during inductive cooling treatment but not subjected control photoperiod in P. aphrodite. Phloem or shoot apex-specific expression of restores late ft mutants. Moreover, can suppress delayed caused SHORT VEGATATIVE PHASE (SVP) overexpression as well an...