A5073944683- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Plant nutrient uptake and metabolism
- Plant-Microbe Interactions and Immunity
- Cellular transport and secretion
- Banana Cultivation and Research
- Seed Germination and Physiology
- Plant Pathogens and Fungal Diseases
- Plant responses to water stress
- Polysaccharides and Plant Cell Walls
- Plant Parasitism and Resistance
- Forest Insect Ecology and Management
- Legume Nitrogen Fixing Symbiosis
- Microtubule and mitosis dynamics
- Plant Micronutrient Interactions and Effects
- Transgenic Plants and Applications
- Metal-Catalyzed Oxygenation Mechanisms
- Lipid metabolism and biosynthesis
- Endoplasmic Reticulum Stress and Disease
- Mycotoxins in Agriculture and Food
- Advanced Proteomics Techniques and Applications
- Plant responses to elevated CO2
Palacký University Olomouc
2014-2024
Czech Academy of Sciences, Biology Centre
2016-2021
University of Bonn
2007-2010
Mississippi State University
2010
Slovak Academy of Sciences
2004-2007
Institute of Plant Genetics and Biotechnology of the Slovak Academy of Sciences
2005
Institute of Botany of the Slovak Academy of Sciences
2004
Summary The role of YODA MITOGEN ACTIVATED PROTEIN KINASE 4 (MAPKKK4) upstream 6 (MPK6) was studied during post‐embryonic root development Arabidopsis thaliana . Loss‐ and gain‐of‐function mutants ( yda1 ΔNyda1 ) were characterized in terms patterning, endogenous auxin content global proteomes. We surveyed morphological cellular phenotypes suggesting possible involvement auxin. Endogenous indole‐3‐acetic acid (IAA) levels up‐regulated both mutants. Proteomic analysis revealed up‐regulation...
Plant hormones cytokinins (CKs) are one of the major mediators physiological responses throughout plant life span. Therefore, a proper homeostasis is maintained by regulation their active levels. Besides degradation, CKs deactivated uridine diphosphate glycosyltransferases (UGTs). Physiologically, levels decline in senescing organs, providing signal to nutrients that shift reproductive tissues has begun. In this work, we show CK glucosides distribution Arabidopsis leaves during developmental...
Small Rab GTPases are important regulators of vesicular trafficking in plants. AtRabA1d, a member the RabA1 subfamily small GTPases, was previously found vesicle-rich apical dome growing root hairs suggesting role during tip growth; however, its specific intracellular localization and plants has not been well described. The transient expression 35S::GFP:RabA1d construct Allium porrum Nicotiana benthamiana revealed structures, which were further corroborated stable transformed Arabidopsis...
Abstract Background Hydroxyproline rich glycoproteins (HRGPs) are implicated to have a role in many aspects of plant growth and development but there is limited knowledge about their localization function during somatic embryogenesis higher plants. In this study, the hydroxyproline embryogenic cells (ECs) embryos banana were investigated by using immunobloting immunocytochemistry with monoclonal JIM11 JIM20 antibodies as well treatment 3,4-dehydro-L-proline (3,4-DHP, an inhibitor extensin...
Wortmannin is a widely used pharmaceutical compound which employed to define vesicular trafficking routes of particular proteins or cellular compounds. It targets phosphatidylinositol 3-kinase and 4-kinases in dose-dependent manner leading the inhibition protein vacuolar sorting endocytosis. Combined proteomics cell biological approaches have been this study explore effects wortmannin on Arabidopsis root cells, especially proteome endomembrane trafficking. On subcellular level, caused...
Superoxide dismutases (SODs) are enzymes detoxifying superoxide to hydrogen peroxide while temporal developmental expression and subcellular localisation linked their functions. Therefore, we aimed here reveal in vivo expression, subcellular, tissue- organ-specific of iron dismutase 1 (FSD1) Arabidopsis using light-sheet Airyscan confocal microscopy. FSD1-GFP temporarily accumulated at the site endosperm rupture during seed germination. In emerged roots, it showed highest abundance cells...
Abstract The forest canopy, as a biodiversity hotspot with many wildlife habitats, remains difficult site to access for researchers and managers. Here, we present novel, small, unmanned aerial vehicle, called the Druid Drone (DD), equipped multifunctional manipulator designed tree crown management research. Its use is demonstrated by studies control of widespread, obligatory hemiparasitic European mistletoe ( Viscum album L.). DD provides integrated pest canopy surveying, spraying sampling...
The generation of reactive oxygen species (ROS) and their regulation by antioxidant enzymes such as IRON SUPEROXIDE DISMUTASE 1 (FSD1) are critical for managing plant responses to salt stress. However, the protein networks modulating ROS levels during stress remain incompletely understood. Our co-immunoprecipitation analysis identified FSD1 an interaction partner scaffolding RECEPTOR FOR ACTIVATED C KINASE 1A (RACK1A). Bimolecular fluorescence complementation analyses revealed that RACK1A...
LY294002 is a synthetic quercetin-like compound, which, unlike wortmannin, more specific inhibitor of phosphatidylinositol 3-kinase (PI3K). It inhibits endocytosis and vacuolar transport. We report here on the proteome-wide effects Arabidopsis roots focusing proteins involved in vesicular trafficking stress response. At subcellular level, caused swelling clustering late endosomes leading to inhibition proteome this compound changes abundances categorized 10 functional classes. Among...
Recent studies suggest that plant pectin methylesterases (PMEs) are directly involved in defence besides their roles development. However, the molecular mechanisms of PME action on pectins not well understood. In order to understand how PMEs modify during banana (Musa spp.)-Fusarium interaction, expression and enzyme activities two cultivars, highly resistant or susceptible Fusarium, were compared with each other. Furthermore, spatial distribution effect methylesterification 10 individual...
Dual-specificity mitogen-activated protein kinases (MAPKKs) are the immediate upstream activators of MAPKs. They simultaneously phosphorylate TXY motif within activation loop MAPKs, allowing them to interact with and regulate multiple substrates. Often, MAPKs triggers their nuclear translocation. However, spatiotemporal dynamics physiological consequences particularly in plants, still poorly understood. Here, we studied localization Medicago sativa stress-induced MAPKK (SIMKK)–SIMK module...
Abstract Banana Fusarium wilt caused by oxysporum f. sp. cubense (Foc ) is soil-borne disease of banana ( Musa spp.) causing significant economic losses. Extensins and arabinogalactan proteins (AGPs) are cell wall components important for pathogen defence. Their significance Foc resistance in was not reported so far. In this study, two cultivars differing sensitivity were used to monitor the changes transcript levels, abundance distribution extensins AGPs after wounding inoculation. mainly...
Microtubule organization and dynamics are critical for key developmental processes such as cell division, elongation, morphogenesis. severing is an essential regulator of microtubules exclusively executed by KATANIN 1 in Arabidopsis. In this study, we comparatively studied the proteome-wide effects two mutants. Thus, shotgun proteomic analysis roots aerial parts single nucleotide mutant fra2 T-DNA insertion ktn1-2 was carried out. We have detected 42 proteins differentially abundant both...
Iron superoxide dismutase 1 (FSD1) was recently characterized as a plastidial, cytoplasmic, and nuclear enzyme with osmoprotective antioxidant functions. However, the current knowledge on its role in oxidative stress tolerance is ambiguous. Here, we of FSD1 response to methyl viologen (MV)-induced Arabidopsis thaliana. In accordance known regulation expression, abundance, activity, findings demonstrated that function depends availability Cu2+ growth media. fsd1 mutants showed lower capacity...
The growing importance of vesicular trafficking and cytoskeleton dynamic reorganization during plant development requires the exploitation novel experimental approaches. Several genetic cell biological studies have used diverse pharmaceutical drugs that inhibit secretion to study these phenomena. Here, proteomic biology approaches were applied effects brefeldin A (BFA), an inhibitor vesicle recycling secretion, in Arabidopsis roots. main aim this was obtain overview proteins affected by BFA,...
Banana Fusarium wilt caused by oxysporum f. sp. cubense (Foc) is one of the most destructive soil-borne diseases. In this study, young tissue-cultured plantlets banana (Musa spp. AAA) cultivars differing in Foc susceptibility were used to reveal their differential responses pathogen using digital gene expression (DGE). Data evaluated various bioinformatic tools (Venn diagrams, ontology (GO) annotation and Kyoto encyclopedia genes genomes (KEGG) pathway analyses) immunofluorescence labelling...