A5083726443- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Antioxidant Activity and Oxidative Stress
- Plant Pathogens and Fungal Diseases
- Metal-Catalyzed Oxygenation Mechanisms
- Plant-Microbe Interactions and Immunity
- Mycotoxins in Agriculture and Food
- Plant responses to water stress
- Mitochondrial Function and Pathology
- Vitamin C and Antioxidants Research
- Plant Micronutrient Interactions and Effects
- Redox biology and oxidative stress
Palacký University Olomouc
2021-2023
Czech Academy of Sciences, Biology Centre
2021
The generation of reactive oxygen species (ROS) and their regulation by antioxidant enzymes such as IRON SUPEROXIDE DISMUTASE 1 (FSD1) are critical for managing plant responses to salt stress. However, the protein networks modulating ROS levels during stress remain incompletely understood. Our co-immunoprecipitation analysis identified FSD1 an interaction partner scaffolding RECEPTOR FOR ACTIVATED C KINASE 1A (RACK1A). Bimolecular fluorescence complementation analyses revealed that RACK1A...
Iron superoxide dismutase 1 (FSD1) was recently characterized as a plastidial, cytoplasmic, and nuclear enzyme with osmoprotective antioxidant functions. However, the current knowledge on its role in oxidative stress tolerance is ambiguous. Here, we of FSD1 response to methyl viologen (MV)-induced Arabidopsis thaliana. In accordance known regulation expression, abundance, activity, findings demonstrated that function depends availability Cu2+ growth media. fsd1 mutants showed lower capacity...
The roles of mitogen-activated protein kinases (MAPKs) in plant-fungal pathogenic interactions are poorly understood crops. Here, microscopic, phenotypic, proteomic, and biochemical analyses revealed that roots independent transcription activator-like effector nuclease (TALEN)-based knockout lines barley (Hordeum vulgare L.) MAPK 3 (HvMPK3 KO) were resistant against Fusarium graminearum infection. When co-cultured with the HvMPK3 KO lines, F. hyphae excluded to extracellular space, growth...
Abstract The photosynthesis-induced accumulation of reactive oxygen species in chloroplasts can lead to oxidative stress, triggering changes protein synthesis, degradation, and the assembly/disassembly complexes. Using shot-gun proteomics, we identified methyl viologen-induced abundance wild-type Arabidopsis stress-hypersensitive fsd1-1 fsd1-2 knockout mutants, which are deficient IRON SUPEROXIDE DISMUTASE 1 (FSD1). levels proteins that localized cytoplasm were modified all lines treated...
Abstract Iron superoxide dismutase 1 (FSD1) was recently characterized as a plastidial, cytoplasmic, and nuclear with osmoprotective antioxidative functions. However, its role in oxidative stress tolerance is not well understood. Here, we the of FSD1 response to methyl viologen (MV)-induced Arabidopsis thaliana . The findings demonstrated that function depends on availability Cu 2+ growth media. Prolonged MV exposure led decreased accumulation rate superoxide, higher levels hydrogen peroxide...
Changing environmental conditions cause rapid accumulation of reactive oxygen species, which can generate oxidative stress in plants.Modulation protein synthesis, degradation and assembly or disassembly complexes represent primary mechanisms plant responses to stress.Our objective was delineate early changes abundances associated with the response Arabidopsis methyl viologen (MV)-induced using shot-gun proteomics.Wild type (WT) plants have been compared stress-hypersensitive fsd1-1 fsd1-2...
Abstract The roles of mitogen-activated protein kinases (MAPKs) in plant-fungal pathogenic interactions are less understood crops. Here, microscopic, phenotyping, proteomic and biochemical analyses revealed that independent TALEN-based knockout lines Hordeum vulgare MITOGEN-ACTIVATED PROTEIN KINASE 3 ( HvMPK3 KO) were resistant against Fusarium graminearum infection. When co-cultured with roots the KO lines, F. hyphae excluded to extracellular space, growth pattern was considerably...