A5005749982- Plant Molecular Biology Research
- Plant Reproductive Biology
- Polysaccharides and Plant Cell Walls
- Photosynthetic Processes and Mechanisms
- Plant and Biological Electrophysiology Studies
- Heavy metals in environment
- Plant Stress Responses and Tolerance
- Lichen and fungal ecology
- Photoreceptor and optogenetics research
- Botany and Plant Ecology Studies
- Cellular transport and secretion
- Radioactive contamination and transfer
- Aluminum toxicity and tolerance in plants and animals
- Mycorrhizal Fungi and Plant Interactions
- Plant and animal studies
- Plant Parasitism and Resistance
- Radioactivity and Radon Measurements
- Plant-Microbe Interactions and Immunity
- Silicon Effects in Agriculture
- Microtubule and mitosis dynamics
- Marine Invertebrate Physiology and Ecology
- Metal complexes synthesis and properties
- Protist diversity and phylogeny
- Plant nutrient uptake and metabolism
- Plant Micronutrient Interactions and Effects
University of Vienna
2012-2021
ABSTRACT Structural observations provide persuasive evidence for the existence of a cortical network endoplasmic reticulum (ER) in large number plant and animal cells. The plants generally possesses polygonal pattern which smooth, tubular elements are joined by intervening lamellar segments. individual ER often positioned extremely close to plasma membrane (PM), may form appositional contacts, but fusion does not occur. arises at cytokinesis establishes continuity between daughter cells...
The extent of aluminium internalization during the recovery from stress in living roots Arabidopsis thaliana was studied by non-invasive vivo microscopy real time. Aluminium exposure caused rapid depolarization plasma membrane. depends on developmental state root cells; it much more extensive cells distal than proximal portion transition zone. Also full membrane potential after removal external slower zone its part. Using morin, a vital marker dye for aluminium, and FM4-64,...
NanoSIMS combined with fluorescence microscopy reveals subcellular structures in cancer cells where 15 N-labeled cisplatin is accumulated, implications for Pt–N bond integrity.
Tomato is a globally important crop grown and consumed worldwide. Its reproductive activity highly sensitive to environmental fluctuations, for instance temperature drought. Here, pollen development one of the most decisive processes. The present study aims identification cell-specific proteins during developmental stages tomato. We have setup protocol stage-specific isolation including microsporocytes (pollen mother cells), tetrads, microspores, polarized mature pollen. Proteins were...
Triapine (3-aminopyridine-2-carboxaldehyde thiosemicarbazone), which entered several phase I and II clinical trials as an antitumor chemotherapeutic agent, was found to possess intrinsic fluorescence properties (lambda(ex) = 360 nm), enabled us monitor the uptake intracellular distribution in living human cancer cells by microscopy.
Abstract Structural sterols are abundant in the plasma membrane of root apex cells Arabidopsis thaliana. They specifically accumulate trichoblasts during prebulging and bulge stages show a polar accumulation tip hair elongation but distributed evenly mature hairs. Thus, structural may serve as marker for initiation growth. In addition, they predict branching events mutants with were detected using sterol complexing fluorochrome filipin. Application filipin caused rapid,...
Phosphatidic acid (PA) is an important intermediate in membrane lipid metabolism that acts as a key component of signaling networks, regulating the spatio-temporal dynamics endomembrane system and cytoskeleton. Using tobacco pollen tubes model, we addressed effects PA by probing functions three most relevant enzymes regulate production degradation PA, namely, phospholipases D (PLD), diacylglycerol kinases (DGKs), phosphate phosphatases (LPPs). Phylogenetic analysis indicated highly dynamic...
Date palm (Phoenix dactylifera) can accumulate as much 1% silicon (Si), but not is known about the mechanisms inherent to this process. Here, we investigated in detail uptake, accumulation and distribution of Si date palms, phylogeny transporter genes plants. We characterized PdNIP2 following heterologous expression Xenopus oocytes used qPCR determine relative genes. Silicon was by light microscopy, scanning electron microscopy coupled with X-ray microanalysis Raman microspectroscopy. proved...
Abstract: A new ELF (enzyme labelled fluorescence) assay was applied to detect phosphatase activity in glandular structures of 47 carnivorous plant species, especially Lentibulariaceae, order understand their digestive activities. We address the following questions: (1) Are phosphatases produced by plants and/or inhabitants traps? (2) Which type hairs/glands is involved production phosphatases? (3) Is this a common feature among or it restricted evolutionarily advanced species? Our results...
Carnivorous plants trap, digest and absorb animals in order to supplement their mineral nutrition. Nutrients absorbed by the plant include different nitrogen species, phosphate, potassium, trace elements small organic compounds. Uptake is usually thought be performed via specific channels, but this study provides evidence that endocytosis involved as well. Traps of carnivorous Nepenthes coccinea, ventrata, Cephalotus follicularis, Drosophyllum lusitanicum, Drosera capensis, Dionaea...
Abstract Hypoxia in solid tumors remains a challenge for conventional cancer therapeutics. As source resistance, metastasis development and drug bioprocessing, it influences treatment results disease outcome. Bioreductive platinum(iv) prodrugs might be advantageous over metal-based therapeutics, as biotransformation reductive milieu, such under hypoxia, is required activation. This study deals with two-step screening of experimental different rates reduction lipophilicity the aim identifying...
The role of endophytic fungi isolated from different populations European Ni hyperaccumulators was investigated in regard to the microorganisms' ability enhance hyperaccumulation Noccaea caerulescens. Effects particular species on adaptation N. caerulescens excess were tested by co-cultivation with single strains fungi. Seven these had a positive effect plant biomass production, whereas two inhibited growth; production inoculated plants significantly compared non-inoculated control....
The morphology and the movement of organelles, in particular endoplasmic reticulum (ER), have been studied using video-enhanced microscopy living pollen tubes Lilium longiflorum Thunb. patterns cytoplasmic streaming grains generally correspond with descriptions by Iwanami (1956). We found, however, that organelles move as single independent elements reflecting highly individual characteristics. apical zone tube, so-called ‘clear’ zone, is populated numerous intensively vibrating small size,...
Thlaspi caerulescens is an important component of specific vegetation on mine wastes in Central Slovakia including those containing high soil concentrations Zn, Pb and Cd Banska Stiavnica Cu Spania Dolina. The population density T. varied both metalliferous non-metalliferous soils. Selective distribution metals was observed caerulescens: remained the roots while Zn were hyperaccumulated leaves. In leaves plants growing soils maximum 19410 mg/kg 80 dry matter 16670 127 matter....
A major question in pollen tube growth planta remains: do the walls form a barrier to interaction with environment? Using cryo-FESEM, we directly assessed 3D construction and porosity of tobacco walls. Fractured mature primary showed 40-50 nm spaced lattice continuous fibers interconnected by short rods wall. These observations agree TEM sectioned In secondary callose wall, for which no structure is visible using TEM, cryo-FESEM also revealed 50 consisting longer fibers, approximately 10-15...
Dual-specificity mitogen-activated protein kinases (MAPKKs) are the immediate upstream activators of MAPKs. They simultaneously phosphorylate TXY motif within activation loop MAPKs, allowing them to interact with and regulate multiple substrates. Often, MAPKs triggers their nuclear translocation. However, spatiotemporal dynamics physiological consequences particularly in plants, still poorly understood. Here, we studied localization Medicago sativa stress-induced MAPKK (SIMKK)–SIMK module...