A5102883846- Ion channel regulation and function
- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Neuropeptides and Animal Physiology
- Nicotinic Acetylcholine Receptors Study
- Gut microbiota and health
- Pharmacological Receptor Mechanisms and Effects
- Wildlife Ecology and Conservation
- Pharmacogenetics and Drug Metabolism
- Plant Molecular Biology Research
- Pain Mechanisms and Treatments
- Immunodeficiency and Autoimmune Disorders
- Avian ecology and behavior
- Aluminum toxicity and tolerance in plants and animals
- Toxic Organic Pollutants Impact
- Adenosine and Purinergic Signaling
- Peroxisome Proliferator-Activated Receptors
- Hormonal Regulation and Hypertension
- SARS-CoV-2 and COVID-19 Research
- Plant Stress Responses and Tolerance
- Diabetes and associated disorders
- Tryptophan and brain disorders
- Hematopoietic Stem Cell Transplantation
- Neurobiology and Insect Physiology Research
- Respiratory viral infections research
Palacký University Olomouc
2014-2025
University of South Florida
2022-2023
Sydney Adventist Hospital
2020
Regional Centre of Advanced Technologies and Materials
2018
Slovak Academy of Sciences
2005-2010
Institute of Chemistry of the Slovak Academy of Sciences
2006-2009
Institute of Botany of the Slovak Academy of Sciences
2006-2009
Leipzig University
1998-2006
Comenius University Bratislava
2002
University of Freiburg
1984-1999
The extent to which tryptophan (Trp) metabolism alterations explain or influence the outcome of inflammatory bowel diseases (IBDs) is still unclear. However, several Trp end-products are essential intestinal homeostasis. Here, we investigated role metabolites from kynurenine pathway.Targeted quantitative metabolomics was performed in two large human IBD cohorts (1069 patients with IBD). Dextran sodium sulphate-induced colitis experiments mice were used evaluate effects identified...
The extent of aluminium internalization during the recovery from stress in living roots Arabidopsis thaliana was studied by non-invasive vivo microscopy real time. Aluminium exposure caused rapid depolarization plasma membrane. depends on developmental state root cells; it much more extensive cells distal than proximal portion transition zone. Also full membrane potential after removal external slower zone its part. Using morin, a vital marker dye for aluminium, and FM4-64,...
Aryl hydrocarbon receptor (AhR) is a critical player in the crosstalk between gut microbiota and its host. However, factors regulating AhR within gut, which complex metabolomic environment, are poorly understood. This study investigates effect of combination metabolites on activation mechanism AhR. activity was evaluated using both luciferase reporter system mRNA levels target genes human cell lines colonic explants. studied by radioligand-binding assay, nuclear translocation...
A relationship between aluminum (Al) toxicity, endocytosis, endosomes and vesicle recycling in the root transition zone has recently been demonstrated. Here importance of filamentous actin (F-actin)-based trafficking for Al tolerance investigating maize varieties differing their sensitivities. More was internalized into tip cells Al-sensitive variety 16x36 than Al-tolerant Cateto. The cytoskeleton were primary targets toxicity tips sensitive variety. Visualization boron-cross-linked...
1. Voltage‐dependent currents of untreated (proliferating) and lipopolysaccharide (LPS)‐treated rat microglial cells in culture were recorded using the whole‐cell patch‐clamp technique. 2. Membrane potentials showed prominent peaks at ‐35 mV ‐70 mV. LPS‐treated alternated between two values. This may be due to a negative slope region I‐V relation resulting zero current potentials. 3. From holding potential mV, hyperpolarizing steps evoked an inwardly rectifying both proliferating cells,...
Gastrointestinal microbes respond to biochemical metabolites that coordinate their behaviors. Here, we demonstrate bacterial indole functions as a multifactorial mitigator of Klebsiella grimontii and oxytoca pathogenicity. These closely related produce the enterotoxins tilimycin tilivalline; cytotoxin-producing strains are causative agent antibiotic-associated hemorrhagic colitis have been associated with necrotizing enterocolitis premature infants. We carbohydrates induce cytotoxin...
1. The perivascular nerves of isolated tail arteries from Wistar rats were stimulated with field pulses (1 Hz, 2 pulses, every min). omega-Conotoxin 10 nmol l-1 depressed neurogenically mediated contractions, but did not influence the contractions to noradrenaline 0.1-0.3 mumol l-1. 2. inhibitory effect omega-conotoxin was concentration-dependent (IC50 = 3.8 l-1). It reach a steady-state during 30 min incubation and could be reversed upon subsequent washout for another 60 min. 3. A gradual...
Plants employ acentrosomal mechanisms to organize cortical microtubule arrays essential for cell growth and differentiation. Using structured illumination microscopy (SIM) adopted the optimal documentation of Arabidopsis (Arabidopsis thaliana) hypocotyl epidermal cells, dynamic microtubules labeled with green fluorescent protein fused microtubule-binding domain mammalian microtubule-associated MAP4 protein-fused alpha tubulin6 were comparatively recorded in wild-type plants mitogen-activated...
Previous studies on immune responses following COVID-19 vaccination in patients with common variable immunodeficiency (CVID) were inconclusive respect to the ability of produce vaccine-specific IgG antibodies, while milder forms primary antibody deficiency such as immunoglobulin isotype or selective have not been studied at all. In this study we examined antigen-specific activation CXCR5-positive and CXCR5-negative CD4 + memory cells also isotype-specific functional CVID compared other...
Implantable Cardiac Monitors (ICMs) are used for long-term monitoring of arrhythmias. BIOMONITOR III is a novel ICM with miniaturized profile, long sensing vector due to flexible antenna, simplified implantation dedicated insertion tool pocket formation and placement in single step, daily automatic Home Monitoring (HM) function.In 47 patients undergoing any indication, 16 investigators at 10 Australian sites assessed handling characteristics the tool, R-wave amplitudes, noise burden, P-wave...
1 Effects of clonidine and α-adrenoceptor antagonists were studied on sympathetic neuroeffector transmission in the mouse vas deferens. The amplitude excitatory junction potentials (e.j.ps) was taken as a measure transmitter release per impulse. 2 At concentration 0.5 μm, prazosin abolished depolarizations evoked by iontophoretically applied noradrenaline, but changed neither spontaneous nor nerve stimulation-evoked e.j.ps. 3 Yohimbine 0.1 rauwolscine μm corynanthine did not change e.j.p....
Targeting the aryl hydrocarbon receptor (AhR) is an emerging therapeutic strategy for multiple diseases (e.g., inflammatory bowel disease). Thermosporothrix hazakensis microbial metabolite 2-(1′H-indole-3′-carbonyl)-thiazole-4-carboxylic acid methyl ester (ITE) a putative AhR endogenous ligand. To improve chemical stability, we synthesized series of ITE mimics. Using in vitro assays, identified 2-(1H-indole-3-carbonyl)-N-methyl thiazole-4-carboxamide (ITE-CONHCH3) as highly potent (EC50 =...
Aryl hydrocarbon receptor (AHR) plays pivotal roles in intestinal physiology and pathophysiology. Intestinal AHR is activated by numerous dietary, endogenous, microbial ligands. Whereas the effects of individual compounds on are mostly known, real physiological mixtures occurring intestine have not been studied. Using reporter gene assays RT-PCR, we evaluated combinatorial (3520 combinations) 11 catabolites tryptophan (MICTs) AHR. We robustly (n = 30) determined potencies relative efficacies...