A5060553658- Gut microbiota and health
- Streptococcal Infections and Treatments
- Helicobacter pylori-related gastroenterology studies
- Clostridium difficile and Clostridium perfringens research
- IL-33, ST2, and ILC Pathways
- Biochemical and Structural Characterization
- Infective Endocarditis Diagnosis and Management
- Probiotics and Fermented Foods
- Antimicrobial Resistance in Staphylococcus
- Inflammatory Bowel Disease
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Bacterial Infections and Vaccines
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Neonatal and Maternal Infections
- Microscopic Colitis
- Microbial Metabolites in Food Biotechnology
- Gastrointestinal motility and disorders
- Immune cells in cancer
- Cancer Immunotherapy and Biomarkers
- Microtubule and mitosis dynamics
- Dermatology and Skin Diseases
- Erythrocyte Function and Pathophysiology
- Biomedical and Engineering Education
- Bacterial Identification and Susceptibility Testing
Centre de Recherche Saint-Antoine
2018-2025
Inserm
2011-2025
Sorbonne Université
2018-2025
Princip (Czechia)
2025
Fédération Hospitalo-Universitaire, Paris Center for Microbiome Medicine
2021-2024
Assistance Publique – Hôpitaux de Paris
2022-2024
Microbiologie de l’alimentation au service de la santé
2020-2024
AgroParisTech
2021-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2022-2024
Université Paris-Saclay
2022-2024
Interactions between commensal microbes and the immune system are tightly regulated maintain intestinal homeostasis, but little is known about these interactions in humans. We investigated responses of human CD4
Gut interleukin-17A (IL-17)-producing γδ T cells are tissue-resident that involved in both host defense and regulation of intestinal inflammation. However, factors regulate their functions poorly understood. In this study, we find the gut microbiota represses IL-17 production by cecal cells. Treatment with vancomycin, a Gram-positive bacterium-targeting antibiotic, leads to decreased short-chain fatty acids (SCFAs) microbiota. Our data reveal these microbiota-derived metabolites,...
The extent to which tryptophan (Trp) metabolism alterations explain or influence the outcome of inflammatory bowel diseases (IBDs) is still unclear. However, several Trp end-products are essential intestinal homeostasis. Here, we investigated role metabolites from kynurenine pathway.Targeted quantitative metabolomics was performed in two large human IBD cohorts (1069 patients with IBD). Dextran sodium sulphate-induced colitis experiments mice were used evaluate effects identified...
In inflammatory bowel diseases, a breakdown in host microbial interactions accompanies sustained activation of immune cells the gut. Functional studies suggest key role for interleukin-23 (IL-23) orchestrating intestinal inflammation. IL-23 can be produced by various mononuclear phagocytes (MNPs) following acute stimulation, but little is known about cellular sources that drive chronic Here we have addressed this question using physiological model bacteria-driven colitis. By combining...
Interactions between the host and its microbiota are of mutual benefit promote health. Complex molecular pathways underlie this dialog, but identity microbe-derived molecules that mediate mutualistic state remains elusive. Helicobacter hepaticus is a member mouse intestinal tolerated by host. In absence an intact IL-10 signaling, H. induces IL-23-driven inflammatory response in intestine. Here we investigate interactions immune cells may mutualism, molecule(s) involved. Our results show...
Abundance of Faecalibacterium prausnitzii, a dominant bacterium the human microbiota that exhibits antiinflammatory effects, is decreased in patients with inflammatory bowel diseases (IBD). In humans, colonic lamina propria contains IL-10-secreting, Foxp3- Tregs characterized by double expression CD4 and CD8α (DP8α) specificity for F. prausnitzii. This Treg subset IBD. The vivo effect DP8α cells has not been evaluated yet to our knowledge. Here, using humanized model NSG immunodeficient...
Gut microbiota impacts responses to immune checkpoint inhibitors (ICI). A high level of Faecalibacterium prausnitzii have been associated with a positive response ICI in multiple cancer types. Here, based on fecal shotgun metagenomics data, we show two independent cohorts patients non-small cell lung and advanced melanoma that F. at baseline is positively better clinical ICI. In MCA205 tumor-bearing mice, administration strain EXL01, already development for Inflammatory Bowel Disease,...
Inflammatory bowel disease (IBD) results from a combination of genetic predisposition, dysbiosis the gut microbiota and environmental factors, leading to alterations in gastrointestinal immune response chronic inflammation. Caspase recruitment domain 9 (Card9), one IBD susceptibility genes, has been shown protect against intestinal inflammation fungal infection. However, cell types mechanisms involved CARD9 protective role remain unknown.We used dextran sulfate sodium (DSS)-induced adoptive...
Abstract Background Effects of antibiotics on gut bacteria have been widely studied, but very little is known about the consequences such treatments fungal microbiota (mycobiota). It commonly believed that load increases in gastrointestinal tract following antibiotic treatment, better characterization clearly needed how directly or indirectly affect mycobiota and thus entire microbiota. Design We used samples from humans (infant cohort) mice (conventional human microbiota-associated mice) to...
Background. Streptococcus gallolyticus is a causative agent of infective endocarditis associated with colon cancer. Genome sequence strain UCN34 revealed the existence 3 pilus loci (pil1, pil2, and pil3). Pili are long filamentous structures playing key role as adhesive organelles in many pathogens. The pil1 locus encodes 2 LPXTG proteins (Gallo2178 Gallo2179) 1 sortase C (Gallo2177). Gallo2179 displaying functional collagen-binding domain was referred to adhesin, whereas Gallo2178...
Streptococcus gallolyticus is an increasing cause of bacteremia and infective endocarditis in the elderly. Several epidemiological studies have associated presence this bacterium with colorectal cancer. We studied interaction S. human colonic cells. strain UCN34, adhered better to mucus-producing cells such as HT-29-MTX than parental HT-29 Attachment mucus dependent on pil3 pilus operon, which heterogeneously expressed wild-type UCN34 population. constructed a deletion mutant Pil3...
Abstract Background Innate immunity genes have been reported to affect susceptibility inflammatory bowel diseases (IBDs) and colitis in mice. Dectin-1, a receptor for fungal cell wall β-glucans, has clearly implicated gut microbiota modulation modification of the inflammation. Here, we explored role Dectin-1 Dectin-2 (another molecules) deficiency intestinal Design Susceptibility dextran sodium sulfate (DSS)-induced was assessed wild-type, knockout (KO), Dectin-2KO, double Dectin-1KO...
The etiology of inflammatory bowel disease (IBD) is unclear but involves both genetics and environmental factors, including the gut microbiota. Indeed, exacerbated activation gastrointestinal immune system toward microbiota occurs in genetically susceptible hosts under influence environment. For instance, a majority IBD susceptibility loci lie within genes involved responses, such as caspase recruitment domain member 9 (Card9). However, relative impacts genotype versus on colitis context...
The gut microbiome is significantly altered in inflammatory bowel diseases, but the basis of these changes not well understood. We have combined metagenomic and metatranscriptomic profiling to assess modifications both bacterial community structure transcriptional activity a mouse model colitis. By using transcriptomic analysis colonic tissue luminal RNA derived from host, we also characterised how host transcription relates microbial response inflammation. In colitis, increased abundance...
Inflammatory bowel disease (IBD) is a chronic inflammatory condition with increasing prevalence. Current therapies are only partially efficient and associated potentially severe side effects, highlighting the need for innovative therapeutic strategies. Here, we explored novel dialogue between neutrophils microbiota in intestinal homeostasis inflammation, opening promising avenue IBD management.1 complex, resulting from genetic predisposition, alterations of gut microbiota, environmental...
Abstract Background and aims Inflammatory Bowel Disease (IBD) are chronic inflammatory conditions of the gastrointestinal tract with increasing prevalence. Current therapies only partially efficient associated potentially severe side effects. New therapeutic strategies innovative targets thus urgently needed. Neutrophils key players innate immunity in IBD, but their roles disease development neglected compared to those other immune cells. Essential host defence, overactivation can cause...
Abstract Background Faecalibacterium prausnitzii, a highly abundant bacterium in the human gut microbiota, has been linked to overall health and is notably decreased pathological conditions, such as Inflammatory Bowel Disease (IBD). F. prausnitzii shown anti-inflammatory properties mouse models, through induction of IL-10 signaling. Here, we investigated which cell types from blood intestinal tissue are responsible for producing induced by providing first mechanistic insights. Methods Immune...
The gut microbiota constitutes a complex ecosystem essential for host defense against infection and maturation of the immune system. Inflammatory bowel diseases (IBD), such as ulcerative colitis Crohn's disease, are characterized by severe inflammation intestine, arising from dysregulated control host-microbiota crosstalk. However, neither genetic bases IBD nor responses involved fully understood. pathobionts currently under investigation their active role in development severity IBD. These...
Streptococcus gallolyticus is a commensal bacterium responsible for infectious endocarditis in the elderly, which has frequently been associated with colonic carcinoma. Whether this species cause or consequence of colorectal cancer remains unknown. We recently demonstrated that S. Pil3 pilus required adhesion to mucus and colonization mouse distal colon. show here binds equally well human mucins derived from HT29-MTX cells stomach healthy donors. In addition, we have found also fibrinogen,...
Yersinia pestis dissemination in a host is usually studied by enumerating bacteria the tissues of animals sacrificed at different times. This laborious methodology gives only snapshots infection, as infectious process not synchronized. In this work we used vivo bioluminescence imaging (BLI) to follow Y. during bubonic plague. We first demonstrated that CO92 transformed with pGEN-luxCDABE stably emitted vitro and vivo, while retaining full virulence. The light produced from live allowed...