A5076707525- Photosynthetic Processes and Mechanisms
- Lipid metabolism and biosynthesis
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant biochemistry and biosynthesis
- Algal biology and biofuel production
- Legume Nitrogen Fixing Symbiosis
- Polysaccharides and Plant Cell Walls
- Hereditary Neurological Disorders
- Plant Gene Expression Analysis
- Plant Surface Properties and Treatments
- Plant Stress Responses and Tolerance
- Neurogenetic and Muscular Disorders Research
- Plant-Microbe Interactions and Immunity
- Bacterial Genetics and Biotechnology
- Microbial Metabolic Engineering and Bioproduction
- RNA Research and Splicing
- Cellular transport and secretion
- Plant responses to water stress
- Plant Parasitism and Resistance
- Mass Spectrometry Techniques and Applications
- Cerebral Palsy and Movement Disorders
- Enzyme Structure and Function
- Weed Control and Herbicide Applications
University of Göttingen
2016-2025
Institute of Plant Biology and Biotechnology
2022-2024
University of Münster
2021-2024
Biotechnology Institute
2022
Universitätsmedizin Göttingen
2018
University of Vienna
2012-2013
Brookhaven National Laboratory
2007
Max Planck Society
2005
The turnover of brain proteins is critical for organism survival, and its perturbations are linked to pathology. Nevertheless, protein lifetimes have been difficult obtain in vivo. They readily measured vitro by feeding cells with isotopically labeled amino acids, followed mass spectrometry analyses. In vivo generated from at least two sources: acids the diet, non-labeled degradation pre-existing proteins. This renders measurements difficult. Here we solved this problem rigorously a workflow...
Plant terrestrialization brought forth the land plants (embryophytes). Embryophytes account for most of biomass on and evolved from streptophyte algae in a singular event. Recent advances have unravelled first full genomes closest algal relatives plants; among such species was Mesotaenium endlicherianum. Here we used fine-combed RNA sequencing tandem with photophysiological assessment exposed to continuous range temperature light cues. Our data establish grid 42 different conditions,...
Abstract Phosphatidylinositol-4,5-bisphosphate [PtdIns(4,5)P2] occurs in the apical plasma membrane of growing pollen tubes. Because enzymes responsible for PtdIns(4,5)P2 production at that location are uncharacterized, functions tube tip growth unresolved. Two candidate genes encoding pollen-expressed Arabidopsis thaliana phosphatidylinositol-4-phosphate 5-kinases (PI4P 5-kinases) subfamily B were identified (PIP5K4 and PIP5K5), their recombinant proteins characterized as being PI4P...
Abstract Root hairs are extensions of root epidermal cells and a model system for directional tip growth plant cells. A previously uncharacterized Arabidopsis thaliana phosphatidylinositol-4-phosphate 5-kinase gene (PIP5K3) was identified found to be expressed in the cortex, cells, hairs. Recombinant PIP5K3 protein catalytically active converted phosphatidylinositol-4,5-bisphosphate [PtdIns(4,5)P2]. mutant plants homozygous T-DNA–disrupted alleles were compromised hair formation, phenotype...
The functions of the minor phospholipid phosphatidylinositol-4,5-bisphosphate [PtdIns(4,5)P2] during vegetative plant growth remain obscure. Here, we targeted two related phosphatidylinositol 4-phosphate 5-kinases (PI4P 5-kinases) PIP5K1 and PIP5K2, which are expressed ubiquitously in Arabidopsis thaliana. A pip5k1 pip5k2 double mutant with reduced PtdIns(4,5)P2 levels showed dwarf stature phenotypes suggesting defects auxin distribution. roots had normal but transport altered...
Understanding cellular life requires a comprehensive knowledge of the essential functions, components involved, and their interactions. Minimized genomes are an important tool to gain this knowledge. We have constructed strains model bacterium, Bacillus subtilis , whose been reduced by ∼36%. These fully viable, growth rates in complex medium comparable those wild type strains. An in-depth multi-omics analysis genome revealed how deletions affect transcription regulatory network cell,...
Oleaginous microalgae are considered as a promising resource for the production of biofuels. Especially diatoms arouse interest biofuel producers since they most productive in carbon fixation and very flexible to environmental changes nature. Naturally, triacylglycerol (TAG) accumulation algae only occurs under stress conditions like nitrogen-limitation. We focused on Phaeodactylum strain Pt4 (UTEX 646), because its ability grow medium with low salinity therefore being suited when saline...
SUMMARY Land plants constantly respond to fluctuations in their environment. Part of response is the production a diverse repertoire specialized metabolites. One foremost sources for metabolites relevant environmental responses phenylpropanoid pathway, which was long thought be land‐plant‐specific adaptation shaped by selective forces terrestrial habitat. Recent data have, however, revealed that streptophyte algae, algal relatives land plants, have candidates genetic toolkit biosynthesis and...
The number of known proteins associated with plant lipid droplets (LDs) is small compared other organelles. Many aspects LD biosynthesis and degradation are unknown, identifying characterizing candidate could help elucidate these processes. Here, we analyzed the proteome LD-enriched fractions isolated from tobacco (Nicotiana tabacum) pollen tubes. Proteins that were highly enriched in comparison total or cytosolic fraction further tested for localization via transient expression One...
To maintain homeostasis, the body, including brain, reprograms its metabolism in response to altered nutrition or disease. However, consequences of these challenges for energy different brain cell types remain unknown. Here, we generated a proteome atlas major central nervous system (CNS) from young and adult mice, after feeding therapeutically relevant low-carbohydrate, high-fat ketogenic diet (KD) during neuroinflammation. Under steady-state conditions, CNS prefer distinct modes...
beta-Ketoacyl-acyl carrier protein (ACP) synthase II (KASII) elongates 16:0-ACP to 18:0-ACP in the plastid, where it competes with three other enzymes at first major branch point fatty acid biosynthesis. Despite its key metabolic location, influence of KASII determining seed oil composition remains unclear, part because biochemical consequences fab1-1 mutation were unresolved. Thus, fab1-1, and a newly identified knockout allele, fab1-2, analyzed context hypothesis that modulating activity...
Plants exposed to hyperosmotic stress undergo changes in membrane dynamics and lipid composition maintain cellular integrity avoid leakage. Various plant species respond with transient increases PtdIns(4,5)P(2); however, the physiological role of such is unresolved. The plasma represents outermost barrier between symplast cells its apoplastic surroundings. In present study, spatio-temporal stress-induced phosphoinositides were analysed subcellular fractions Arabidopsis leaves delineate...
The regulation of pollen tube growth by the phospholipid phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P(2) ) is not well understood. Arabidopsis genome encodes two type A phosphatidylinositol-4-phosphate (PI4P) 5-kinases, PIP5K10 and PIP5K11, which are exclusively expressed in produce PtdIns(4,5)P(2) vitro. Fluorescence-tagged PIP5K11 localized to lateral subapical plasma membrane microdomains tobacco tubes a pattern closely resembling distribution PtdIns(4,5)P(2,) with exception...
Tomato is a globally important crop grown and consumed worldwide. Its reproductive activity highly sensitive to environmental fluctuations, for instance temperature drought. Here, pollen development one of the most decisive processes. The present study aims identification cell-specific proteins during developmental stages tomato. We have setup protocol stage-specific isolation including microsporocytes (pollen mother cells), tetrads, microspores, polarized mature pollen. Proteins were...
Cytoplasmic lipid droplets (LDs) are found in all types of plant cells; they derived from the endoplasmic reticulum and function as a repository for neutral lipids, well serving remodelling signalling. However, mechanisms underlying formation, steady-state maintenance turnover LDs, particularly non-seed tissues, relatively unknown. Previously, we showed that LD-associated proteins (LDAPs) family plant-specific, LD surface-associated coat required proper biogenesis LDs homeostasis vegetative...
Summary Tuber and root crops virtually exclusively accumulate storage products in the form of carbohydrates. An exception is yellow nutsedge ( Cyperus esculentus ) which tubers have capacity to store starch triacylglycerols TAG roughly equal amounts. This suggests that a tuber crop can efficiently handle accumulation energy dense oil. From nutritional as well economic aspect, it would be interest utilize high yield or for oil similar nutsedge. The transcription factor WRINKLED 1 from...