A5001790537- Advanced Proteomics Techniques and Applications
- RNA Research and Splicing
- Mitochondrial Function and Pathology
- Lipid Membrane Structure and Behavior
- Mass Spectrometry Techniques and Applications
- Microtubule and mitosis dynamics
- Biotin and Related Studies
- Metabolomics and Mass Spectrometry Studies
- Cellular transport and secretion
- Neuroscience and Neuropharmacology Research
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Alzheimer's disease research and treatments
- Amino Acid Enzymes and Metabolism
- Congenital heart defects research
- Cell Adhesion Molecules Research
- Protist diversity and phylogeny
- RNA regulation and disease
- Neural dynamics and brain function
- Genetic and Kidney Cyst Diseases
- Metabolism and Genetic Disorders
- Photosynthetic Processes and Mechanisms
- RNA modifications and cancer
- Protein Structure and Dynamics
Universitätsmedizin Göttingen
2018-2024
Max Planck Institute for Multidisciplinary Sciences
2022
University of Göttingen
2017-2021
Max Planck Institute for Biophysical Chemistry
2014-2021
Nanoscale Microscopy and Molecular Physiology of the Brain Cluster of Excellence 171 — DFG Research Center 103
2018
Synaptic vesicle recycling has long served as a model for the general mechanisms of cellular trafficking. We used an integrative approach, combining quantitative immunoblotting and mass spectrometry to determine protein numbers; electron microscopy measure organelle numbers, sizes, positions; super-resolution fluorescence localize proteins. Using these data, we generated three-dimensional "average" synapse, displaying 300,000 proteins in atomic detail. The copy numbers involved same step...
The turnover of brain proteins is critical for organism survival, and its perturbations are linked to pathology. Nevertheless, protein lifetimes have been difficult obtain in vivo. They readily measured vitro by feeding cells with isotopically labeled amino acids, followed mass spectrometry analyses. In vivo generated from at least two sources: acids the diet, non-labeled degradation pre-existing proteins. This renders measurements difficult. Here we solved this problem rigorously a workflow...
BCAS1 expression identifies newly formed and actively myelinating oligodendrocytes in development, adulthood, disease.
Aging is a prominent risk factor for neurodegenerative disorders (NDDs); however, the molecular mechanisms rendering aged brain particularly susceptible to neurodegeneration remain unclear. Here, we aim determine link between physiological aging and NDDs by exploring protein turnover using metabolic labeling quantitative pulse-SILAC proteomics. By comparing lifetimes physiologically young adult mice, found that in brains are increased ~20% affects distinct pathways linked NDDs. Specifically,...
NeuroLNC , a previously unknown long ncRNA, regulates neuronal activity by interacting with the neurodegeneration protein TDP-43.
Article10 December 2018Open Access Transparent process Inhibition of CPAP–tubulin interaction prevents proliferation centrosome-amplified cancer cells Aruljothi Mariappan orcid.org/0000-0001-5286-9806 Institute für Humangenetik, Universitätsklinikum Düsseldorf, Heinrich-Heine-Universität, Germany Center for Molecular Medicine the University Cologne, Search more papers by this author Komal Soni Structural Biology, Helmholtz Zentrum München, Neuherberg, Biomolecular NMR at Integrated Protein...
Centrosomes are the major microtubule-organizing centers, consisting of centrioles surrounded by a pericentriolar material (PCM). Centrosomal PCM is spatiotemporally regulated to be minimal during interphase and expands as cells enter mitosis. It unclear how expansion initiated at onset Here, we identify that, in Drosophila, Plk1/Polo kinase phosphorylates conserved centrosomal protein Sas-4 vitro. This phosphorylation appears occur mitosis, enabling Sas-4's localization expand outward from...
Abstract The homeostasis of the proteome depends on tight regulation mRNA and protein abundances, translation rates, lifetimes. Results from several studies prokaryotes or eukaryotic cell cultures have suggested that is connected to, perhaps regulated by, codon sequences. However, this has been little investigated for mammals in vivo . Moreover, link between coding sequences one critical parameter, lifetime, remained largely unexplored, both vitro We tested mouse brain, found percentages...
Abstract A strong focus on sex-related differences has arisen recently in neurobiology, but most investigations brain function vivo, ignoring common experimental models like cultured neurons. few studies have addressed morphological between male and female neurons culture, very works focused functional aspects, especially presynaptic function. To fill this gap, we studied here parameters of synaptic vesicle recycling hippocampal cultures from rats, which are a standard model system for many...
Protein phosphorylation is a critical post-translational modification that orchestrates cellular signaling. Here, we introduce PulsPhos, method combining metabolic labeling with phosphoproteomics, spectral analysis and modeling, to quantify site-specific lifetimes in living cells. Phosphosite vary over multiple orders of magnitude are influenced by factors such as amino acid composition subcellular localization. PulsPhos was readily applied pharmacological perturbations, revealing...