A5085252430- Cellular transport and secretion
- Cell Adhesion Molecules Research
- Neuroscience and Neuropharmacology Research
- Ion-surface interactions and analysis
- Advanced Electron Microscopy Techniques and Applications
- Lipid Membrane Structure and Behavior
- Hereditary Neurological Disorders
- Cellular Mechanics and Interactions
- Supramolecular Self-Assembly in Materials
- Optical Coherence Tomography Applications
- Neuropeptides and Animal Physiology
- Electron and X-Ray Spectroscopy Techniques
- Integrated Circuits and Semiconductor Failure Analysis
- Microtubule and mitosis dynamics
- Glycosylation and Glycoproteins Research
- Advanced Fluorescence Microscopy Techniques
- Advanced biosensing and bioanalysis techniques
- Signaling Pathways in Disease
- Mass Spectrometry Techniques and Applications
- Protease and Inhibitor Mechanisms
- Cell Image Analysis Techniques
- Mitochondrial Function and Pathology
- Retinal Development and Disorders
- Protein Structure and Dynamics
- Alzheimer's disease research and treatments
University of Göttingen
2020-2022
Universitätsmedizin Göttingen
2020-2022
The brain extracellular matrix (ECM) consists of extremely long-lived proteins that assemble around neurons and synapses, to stabilize them. ECM is thought change only rarely, in relation neuronal plasticity, through proteolysis renewed protein synthesis. We report here an alternative remodeling mechanism, based on the recycling molecules. Using multiple labeling imaging assays, from super-resolution optical nanoscale secondary ion mass spectrometry, both culture slices, we find a key...
Neurons are highly asymmetric cells that span long distances and need to react promptly local demands. Consequently, neuronal secretory pathway elements distributed throughout neurites, specifically in post-synaptic compartments, enable protein synthesis delivery. Whether how changes synaptic activity correlate is still unclear. To assess this, we used STED nanoscopy automated quantitative image analysis of markers the endoplasmic reticulum, ER-Golgi intermediate compartment, trans-Golgi...
ABSTRACT Axonal survival and growth requires signalling from tropomyosin receptor kinases (Trks). To transmit their signals, receptor–ligand complexes are endocytosed undergo retrograde trafficking to the soma, where downstream occurs. Vesicles transporting neurotrophic receptors soma reported be Rab7-positive late endosomes and/or multivesicular bodies (MVBs), localize within so-called intraluminal vesicles (herein Rab7 corresponds Rab7A unless specified otherwise). Therefore, one...
Nanoscale imaging with the ability to identify cellular organelles and protein complexes has been a highly challenging subject in secondary ion mass spectrometry (SIMS) of biological samples. This is because only few isotopic tags can be used successfully target specific proteins or organelles. To address this, we generated gold nanoprobes, which nanoparticles are conjugated nanobodies. The nanoprobes were well suited for molecular using NanoSIMS at subcellular resolution. They demonstrated...
Nano secondary ion mass spectrometry (nanoSIMS) imaging is a rapidly growing field in biological sciences, which enables investigators to describe the chemical composition of cells and tissues with high resolution. One major challenges nanoSIMS identify specific molecules or organelles, as these are not immediately recognizable need be revealed by SIMS-compatible probes. Few laboratories have generated such probes, none commercially available. To address this, we performed systematic study...
Summary The brain extracellular matrix (ECM) assembles around neurons and synapses, is thought to change only rarely, through proteolysis renewed protein synthesis. We report here an alternative ECM remodeling mechanism, based on the recycling of molecules. found that a key protein, Tenascin-R, frequently endocytosed, later resurfaces, preferentially near synapses. TNR molecules complete this cycle within ∼3 days, in activity-dependent fashion.
Nanoscale imaging with the ability to identify cellular organelles and protein complexes has been a highly challenging subject in secondary ion mass spectrometry (SIMS) of biological samples. This is because only few isotopic tags can be used successfully target specific proteins or organelles. To address this, we have generated gold-nanoprobes, which gold nanoparticles are conjugated nanobodies. The nanoprobes were well suited for molecular using NanoSIMS at subcellular resolution. They...