A5049277731- Enzyme Structure and Function
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Biochemical and Molecular Research
- Retinal Development and Disorders
- Microtubule and mitosis dynamics
- Protist diversity and phylogeny
- Photoreceptor and optogenetics research
- Genetic and Kidney Cyst Diseases
- Invertebrate Immune Response Mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Tuberculosis Research and Epidemiology
- Cancer-related gene regulation
- RNA and protein synthesis mechanisms
- Plant biochemistry and biosynthesis
- Hydrogen Storage and Materials
- Influenza Virus Research Studies
- Photosynthetic Processes and Mechanisms
- Cellular transport and secretion
- Neurobiology and Insect Physiology Research
- Magnetic and Electromagnetic Effects
- bioluminescence and chemiluminescence research
- RNA Research and Splicing
- Signaling Pathways in Disease
- Plant nutrient uptake and metabolism
Zhengzhou University
2024
Columbia University
2020-2023
Tsinghua University
2010-2019
Center for Life Sciences
2015-2019
Harbin Veterinary Research Institute
2011
Institute of Oceanology
2009
Chinese Academy of Sciences
2009
High-frequency point mutations of genes encoding histones have been identified recently as novel drivers in a number tumors. Specifically, the H3K36M/I were shown to be oncogenic chondroblastomas and undifferentiated sarcomas by inhibiting H3K36 methyltransferases, including SETD2. Here we report crystal structures SETD2 catalytic domain bound H3K36M or H3K36I peptides with SAH (S-adenosylhomocysteine). In complex structure, adopts an open conformation, K36M/I peptide snuggly positioned...
Heterochromatin Protein 1 (HP1) recognizes histone H3 lysine 9 methylation (H3K9me) through its conserved chromodomain and maintains heterochromatin from fission yeast to mammals. However, in Arabidopsis, Like (LHP1) colocalizes genome-wide with H3K27me3, is the functional homolog of Polycomb protein. This raises question whether genuine HP1 homologs exist plants. Here, we report on discovery ADCP1, a plant-specific triple tandem Agenet protein, as multivalent H3K9me reader establish that...
Abstract Centrioles and cilia are microtubule-based structures, whose precise formation requires controlled cytoplasmic tubulin incorporation. How is recognized for centriolar/ciliary-microtubule construction remains poorly understood. Centrosomal-P4.1-associated-protein (CPAP) binds via its PN2-3 domain. Here, we show that a C-terminal loop-helix in targets β-tubulin at the microtubule outer surface, while an N-terminal helical motif caps microtubule’s α-β surface of β-tubulin. Through...
Significance In centrosomes, pericentriolar material (PCM) serves as the principle site for microtubule nucleation and anchoring. Drosophila , centrosomal protein spindle assembly defective-4 (Sas-4) scaffolds cytoplasmic PCM complexes via its N terminus tethers them to centrioles an unknown mechanism. By determining crystal structure of Sas-4‘s C-terminal T complex 10 (TCP) domain functional studies in human cells, induced pluripotent stem cell-derived neural progenitors, we show that Sas-4...
Article10 December 2018Open Access Transparent process Inhibition of CPAP–tubulin interaction prevents proliferation centrosome-amplified cancer cells Aruljothi Mariappan orcid.org/0000-0001-5286-9806 Institute für Humangenetik, Universitätsklinikum Düsseldorf, Heinrich-Heine-Universität, Germany Center for Molecular Medicine the University Cologne, Search more papers by this author Komal Soni Structural Biology, Helmholtz Zentrum München, Neuherberg, Biomolecular NMR at Integrated Protein...
NRMT1 is an N-terminal methyltransferase that methylates histone CENP-A as well nonhistone substrates. Here, we report the crystal structure of human bound to peptide at 1.3 Å. adopts a core fold resembles DOT1L and PRMT but not SET domain family methyltransferases. Key substrate recognition catalytic residues were identified by mutagenesis studies. Histone profiling revealed highly selective fruit fly H2B, which share common “X aa -Pro–Lys/Arg” motif. These results, along with 1.5 Å...
Numerous missense mutations in cyclic nucleotide-gated (CNG) channels cause achromatopsia and retinitis pigmentosa, but the underlying pathogenic mechanisms are often unclear. We investigated structural basis molecular/cellular effects of R410W, an achromatopsia-associated, presumed loss-of-function mutation human CNGA3. Cryo-EM structures Caenorhabditis elegans TAX-4 CNG channel carrying analogous mutation, R421W, show that most apo open. R421, located gating ring, interacts with S4 segment...
Background It was proposed that there are at least 250 enzymes in M. tuberculosis involved lipid metabolism. Rv0045c predicted to be a hydrolase by amino acid sequence similarity, although its precise biochemical characterization and function remained defined. Methodology/Principal Findings We expressed the protein high levels E. coli purified purity. confirmed prepared mass spectrometry analysis. Circular dichroism spectroscopy analysis showed possessed abundant β-sheet secondary structure,...
There are at least 250 enzymes in Mycobacterium tuberculosis (M. tuberculosis) involved lipid metabolism. Some of the required for bacterial survival and full virulence. The esterase Rv0045c shares little amino acid sequence similarity with other members esterase/lipase family. Here, we report 3D structure Rv0045c. Our studies demonstrated that is a novel member α/β hydrolase fold contains two distinct domains: domain cap domain. active site highly conserved comprised residues: Ser154...
Abstract Cyclic nucleotide-gated (CNG) channels transduce chemical signals into electrical in sensory receptors and neurons. They are activated by cGMP or cAMP, which bind to the cyclic nucleotide-binding domain (CNBD) open a gate located 50-60 Å away central cavity. Structures of closed vertebrate CNG have been solved, but conformational landscape this allosteric gating remains be elucidated enriched. Here, we report structures cGMP-activated human cone photoreceptor CNGA3/CNGB3 channel...
Cecropin B is a 37-residue cationic antimicrobial peptide derived from the haemolymph of Bombyx mori. The precise mechanism by which cecropins exert their and cytolytic activities not well understood. Crystals cecropin were obtained hanging-drop vapour-diffusion method using polyethylene glycol as precipitant at 289 K. crystal diffracted to 1.43 A resolution X-ray radiation belonged orthorhombic space group P1, with unit-cell parameters = 15.08, b 22.75, c 30.20 A, alpha 96.9, beta 103.1,...
The Rv0045c protein is predicted to be an esterase that involved in lipid metabolism Mycobacterium tuberculosis. was overproduced Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. crystals diffracted a resolution of 2.7 Å synchrotron-radiation source belonged space group P3(1) or P3(2), with unit-cell parameters a=b=73.465, c=48.064 Å, α=β=90, γ=120°. Purified SeMet-labelled also formed 3.0 in-house X-ray radiation source.