A5035353106- Retinal Development and Disorders
- Nicotinic Acetylcholine Receptors Study
- Ion channel regulation and function
- Photoreceptor and optogenetics research
- Piperaceae Chemical and Biological Studies
- Neurobiology and Insect Physiology Research
- RNA and protein synthesis mechanisms
- Neuroscience and Neural Engineering
- Connective Tissue Growth Factor Research
- Calcium signaling and nucleotide metabolism
- Cholinesterase and Neurodegenerative Diseases
- Signaling Pathways in Disease
- Ion Channels and Receptors
- Neuroscience and Neuropharmacology Research
- Electrochemical Analysis and Applications
- bioluminescence and chemiluminescence research
Columbia University
2020-2024
University of California, Los Angeles
2016
Numerous missense mutations in cyclic nucleotide-gated (CNG) channels cause achromatopsia and retinitis pigmentosa, but the underlying pathogenic mechanisms are often unclear. We investigated structural basis molecular/cellular effects of R410W, an achromatopsia-associated, presumed loss-of-function mutation human CNGA3. Cryo-EM structures Caenorhabditis elegans TAX-4 CNG channel carrying analogous mutation, R421W, show that most apo open. R421, located gating ring, interacts with S4 segment...
Abstract Cyclic nucleotide-gated (CNG) channels transduce chemical signals into electrical in sensory receptors and neurons. They are activated by cGMP or cAMP, which bind to the cyclic nucleotide-binding domain (CNBD) open a gate located 50-60 Å away central cavity. Structures of closed vertebrate CNG have been solved, but conformational landscape this allosteric gating remains be elucidated enriched. Here, we report structures cGMP-activated human cone photoreceptor CNGA3/CNGB3 channel...