A5034202534- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Trace Elements in Health
- Bacterial Genetics and Biotechnology
- Iron Metabolism and Disorders
- Cardiac electrophysiology and arrhythmias
- Advanced Photocatalysis Techniques
- Ion Transport and Channel Regulation
- Drug Transport and Resistance Mechanisms
- Nicotinic Acetylcholine Receptors Study
- Hemoglobinopathies and Related Disorders
- Electrocatalysts for Energy Conversion
- Polysaccharides and Plant Cell Walls
- Neuroscience and Neural Engineering
- Molecular Sensors and Ion Detection
- Phytochemical compounds biological activities
- Cholinesterase and Neurodegenerative Diseases
- Biopolymer Synthesis and Applications
- Natural product bioactivities and synthesis
- RNA and protein synthesis mechanisms
- Alzheimer's disease research and treatments
- Probiotics and Fermented Foods
- Microbial bioremediation and biosurfactants
- Chromatography in Natural Products
- Microbial Fuel Cells and Bioremediation
Baylor College of Medicine
2015-2024
Henan University of Technology
2021-2023
Ocean University of China
2021-2023
China Medical University
2023
Tianjin University
2019-2020
Zhengzhou University
2020
Dalian University
2018-2019
Dalian University of Technology
2018-2019
Shanghai Jiao Tong University
2015-2017
Columbia University
2008-2013
Abstract Ferroportin is an iron exporter essential for releasing cellular into circulation. inhibited by a peptide hormone, hepcidin. In humans, mutations in ferroportin lead to diseases that are often associated with accumulation of macrophages and symptoms deficiency anemia. Here we present the structures from primate Philippine tarsier (TsFpn) presence absence hepcidin solved cryo-electron microscopy. TsFpn composed two domains resembling clamshell structure defines metal ion binding...
Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in mammalian central nervous system and exerts its actions via both ionotropic (GABA(A)) channels metabotropic (GABA(B)) receptors. GABA(A) are ubiquitously expressed neuronal tissues, mature neurons modulate an inward chloride current resulting inhibition due to membrane hyperpolarization. In airway smooth muscle (ASM) cells, hyperpolarization favors relaxation. Although GABA(B) receptors have been functionally...
As an adaptation to infrequent access water, terrestrial mammals produce urine that is hyperosmotic plasma. To prevent osmotic diuresis by the large quantity of urea generated protein catabolism, kidney epithelia contain facilitative transporters (UTs) allow rapid equilibration between urinary space and interstitium. Here we report first X-ray crystal structure a mammalian UT, UT-B, at resolution 2.36 Å. UT-B homotrimer each protomer contains conduction pore with narrow selectivity filter....
Ferroportin (Fpn) is a transporter that releases ferrous ion (Fe 2+ ) from cells and important for homeostasis of iron in circulation. Export one Fe by Fpn coupled to import two H + maintain charge balance. Here, we show human (HsFpn) binds mediates Ca transport. We determine the structure -bound HsFpn identify single binding site distinct sites. Further studies validate transport not another ion. In addition, significantly inhibited presence but vice versa. Function as uniporter may allow...
The Shaker family voltage-dependent potassium channels (Kv1) assemble with cytosolic beta-subunits (Kvbeta) to form a stable complex. All Kvbeta subunits have conserved core domain, which in one of them (Kvbeta2) is an aldoketoreductase that utilizes NADPH as cofactor. In addition this core, Kvbeta1 has N terminus closes the channel by N-type inactivation mechanism. Point mutations putative catalytic site alter on-rate inactivation. Whether functions enzyme and whether its enzymatic activity...
Adaptor proteins are likely to modulate spatially and temporally the trafficking of a number membrane proteins, including neuronal nicotinic acetylcholine receptors (nAChRs). A yeast two-hybrid screen identified novel UBX-containing protein, UBXD4, as one cytosolic that interact directly with α3 α4 nAChR subunits. The function is largely unknown. Immunoprecipitation confocal microscopy confirmed interaction UBXD4 α3-containing nAChRs (α3* nAChRs) expressed in HEK293 cells, PC12 rat cortical...
TrkH is a bacterial ion channel implicated in K+ uptake and pH regulation. assembles with its regulatory protein, TrkA, which closes the when bound to ADP opens it ATP. However, unknown how nucleotides control gating of through TrkA. Here we report structures TrkH-TrkA complex presence or TrkA forms tetrameric ring constrains closed conformation. The splits into two dimers ATP releases constraints on TrkH, resulting an open Functional studies show that both tetramer-to-dimer conversion loss...
Ferroportin (Fpn) is the only known iron exporter in humans and essential for maintaining homeostasis. Fpn activity suppressed by hepcidin, an endogenous peptide hormone, which inhibits export promotes endocytosis of Fpn. Hepcidin deficiency leads to hemochromatosis iron-loading anemia. Previous studies have shown that small peptides mimic first few residues i.e., minihepcidins, are more potent than hepcidin. However, mechanism enhanced inhibition minihepcidins remains unclear. Here, we...
The Kv1 family voltage-dependent K + channels assemble with cytosolic β subunits (Kvβ), which are composed of a flexible N terminus followed by structured core domain. certain Kvβs inactivates the channel blocking ion conduction pore, and domain is functional enzyme that uses NADPH as cofactor. Oxidation Kvβ-bound inhibits inactivation potentiates current, but mechanism behind this effect unknown. Here we show after oxidation, binds to part terminus, thus restraining it from channel....