A5068900946- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Amino Acid Enzymes and Metabolism
- Pesticide and Herbicide Environmental Studies
- Weed Control and Herbicide Applications
- CRISPR and Genetic Engineering
- Probiotics and Fermented Foods
- Microbial Metabolic Engineering and Bioproduction
- GABA and Rice Research
- Polyamine Metabolism and Applications
- Bacterial biofilms and quorum sensing
- Plant nutrient uptake and metabolism
- Biotin and Related Studies
- Evolution and Genetic Dynamics
- Biochemical and Molecular Research
- Metabolism and Genetic Disorders
- Protein Structure and Dynamics
- Microbial Metabolism and Applications
- Microbial infections and disease research
- Plant tissue culture and regeneration
- Listeria monocytogenes in Food Safety
- Legume Nitrogen Fixing Symbiosis
University of Hohenheim
2022-2025
University of Göttingen
2013-2024
Brandenburg University of Technology Cottbus-Senftenberg
2020-2024
Siemens (Germany)
2024
General Department of Preventive Medicine
2015
DSM (Netherlands)
2015
Deutsches Zentrum für Luft- und Raumfahrt e. V. (DLR)
2013
Centro Nacional de Biotecnología
2013
Czech Academy of Sciences, Institute of Microbiology
2010
The second messenger cyclic di-adenosine monophosphate (c-di-AMP) is essential in the Gram-positive model organism Bacillus subtilis and related pathogenic bacteria. It controls activity of conserved ydaO riboswitch several proteins involved potassium (K+) uptake. We found that YdaO protein was among different bacteria provide evidence functions as a K+ transporter. Thus, we renamed gene KimA (K+ importer A). Reporter assays indicated expression beyond c-di-AMP-responsive kimA upstream...
Glycolysis is one of the most important metabolic pathways in heterotrophic organisms. Several genes encoding glycolytic enzymes are essential many bacteria even under conditions when neither nor gluconeogenic activities required. In this study, a screening for vivo interaction partners soil bacterium Bacillus subtilis was used to provide rationale essentiality enzymes. Glycolytic proved be close contact with several other proteins, among them high proportion proteins. Among these partners,...
Understanding cellular life requires a comprehensive knowledge of the essential functions, components involved, and their interactions. Minimized genomes are an important tool to gain this knowledge. We have constructed strains model bacterium, Bacillus subtilis , whose been reduced by ∼36%. These fully viable, growth rates in complex medium comparable those wild type strains. An in-depth multi-omics analysis genome revealed how deletions affect transcription regulatory network cell,...
The control of mRNA stability is an important component regulation in bacteria. Processing and degradation mRNAs are initiated by endonucleolytic attack, the cleavage products processively degraded exoribonucleases. In many bacteria, these RNases, as well RNA helicases other proteins, organized a protein complex called degradosome. Escherichia coli, degradosome assembled around essential endoribonuclease E. Bacillus subtilis, recently discovered RNase Y involved initiation degradation....
Aminophosphonates, like glyphosate (GS) or metal chelators such as ethylenediaminetetra(methylenephosphonic acid) (EDTMP), are released on a large scale worldwide. Here, we have characterized bacterial strain capable of degrading synthetic aminophosphonates. The was isolated from LC/MS standard solution. Genome sequencing indicated that the belongs to genus Ochrobactrum. Whole-genome classification using pyANI software compute pairwise ANI and other metrics between Brucella assemblies...
Glutamate is a central metabolite in all organisms since it provides the link between carbon and nitrogen metabolism. In Bacillus subtilis, glutamate synthesized exclusively by synthase, can be degraded dehydrogenase. B. major dehydrogenase RocG expressed only presence of arginine, bacteria are unable to utilize as source. addition rocG, second cryptic gene (gudB) encodes an inactive Mutations rocG result rapid accumulation gudB1 suppressor mutations that code for active enzyme. this work,...
Genome annotation and access to information from large-scale experimental approaches at the genome level are essential improve our understanding of living cells organisms. This is even more case for model organisms that basis study pathogens technologically important species. We have generated SubtiWiki, a database Gram-positive bacterium Bacillus subtilis (http://subtiwiki.uni-goettingen.de/). In addition established companion modules SubtiPathways SubtInteract, we now created SubtiExpress,...
The recently identified second messenger cyclic di-AMP (c-di-AMP) is involved in several important cellular processes, such as cell wall metabolism, maintenance of DNA integrity, ion transport, transcription regulation, and allosteric regulation enzyme function. Interestingly, c-di-AMP essential for growth the Gram-positive model bacterium Bacillus subtilis. Although genome B. subtilis encodes three c-di-AMP-producing diadenlyate cyclases that can functionally replace each other,...
In a Mars exploration scenario, knowing if and how highly resistant Bacillus subtilis spores would survive on the Martian surface is crucial to design planetary protection measures avoid false positives in life-detection experiments. Therefore, this study systematic screening was performed determine whether B. could an average day Mars. For that, from two comprehensive sets of isogenic mutant strains, defective DNA or repair genes, were exposed 24 h simulated atmospheric environment with...
Abstract The detection and analysis of protein–protein interactions is one the central tasks proteomics in postgenomic era. For this purpose, we present a procedure, Strep–protein interaction experiment (SPINE) that combines advantages Strep‐tag protein purification system with those reversible vivo crosslinking by formaldehyde. Using two Bacillus subtilis regulator proteins, demonstrate method well suited to isolate complexes high purity virtually no background. Plasmids allowing high‐level...
Summary Glutamate synthesis is the link between carbon and nitrogen metabolism . In Bacillus subtilis , glutamate exclusively synthesized by synthase encoded gltAB operon. The dehydrogenase RocG from B. devoted to degradation rather than its synthesis. expression of operon induced glucose ammonium strongly repressed arginine. Regulation arginine depends on transcriptional activator protein GltC. constitutively expressed in a rocG mutant strain, but molecular mechanism negative control has so...