A5046701033- Photosynthetic Processes and Mechanisms
- Pharmaceutical and Antibiotic Environmental Impacts
- Antioxidant Activity and Oxidative Stress
- Environmental Toxicology and Ecotoxicology
- Advancements in Transdermal Drug Delivery
- Pesticide and Herbicide Environmental Studies
- Marine and coastal ecosystems
- Wastewater Treatment and Nitrogen Removal
- Reproductive biology and impacts on aquatic species
- Toxic Organic Pollutants Impact
- Pesticide Residue Analysis and Safety
- Contact Dermatitis and Allergies
- Lipid metabolism and biosynthesis
- Weed Control and Herbicide Applications
- Membrane Separation Technologies
- Coastal and Marine Management
- Museums and Cultural Heritage
- Isotope Analysis in Ecology
- Oil Spill Detection and Mitigation
- Per- and polyfluoroalkyl substances research
- Plant biochemistry and biosynthesis
- Atmospheric chemistry and aerosols
- Food Quality and Safety Studies
- Pharmacological Effects of Natural Compounds
- Marine and environmental studies
Leibniz Institute for Baltic Sea Research
2019-2023
Max Planck Institute of Molecular Plant Physiology
2005-2009
Max Planck Society
2005-2006
Dongseo University
2005
Chloroplasts contain lipoprotein particles termed plastoglobules. Plastoglobules are generally believed to have little function beyond lipid storage. Here we report on the identification of plastoglobule proteins using mass spectrometry methods in Arabidopsis thaliana. We demonstrate specific association members plastid lipid-associated proteins/fibrillin family as well known metabolic enzymes, including tocopherol cyclase (VTE1), a key enzyme (vitamin E) synthesis. Moreover, comparative...
Tocopherol belongs to the Vitamin E class of lipid soluble antioxidants that are essential for human nutrition. In plants, tocopherol is synthesized in plastids where it protects membranes from oxidative degradation by reactive oxygen species. cyclase (VTE1) catalyzes penultimate step synthesis, and an Arabidopsis (Arabidopsis thaliana) mutant deficient VTE1 (vte1) totally devoid tocopherol. Overexpression resulted increase total at least 7-fold leaves, a dramatic shift alpha-tocopherol...
One important reaction of chlorophyll (chl) breakdown during plant senescence is the removal lipophilic phytol moiety by chlorophyllase. AtCLH1 and AtCLH2 were considered to be required for this in Arabidopsis thaliana . Here we present evidence against assumption. Using green fluorescent protein fusions, neither AtCLH isoform localizes chloroplasts, predicted site breakdown. Furthermore, clh1 clh2 single double knockout lines are still able degrade senescence. From our data conclude that...
Plastoglobules, lipid-protein bodies in the stroma of plant chloroplasts, are enriched non-polar lipids, particular prenyl quinols. In present study we show that, addition to thylakoids, plastoglobules also contain a considerable proportion plastidial PQ-9 (plastoquinol-9), redox component photosystem II, and cyclized product PQ-9, PC-8 (plastochromanol-8), tocochromanol with structure similar gamma-tocopherol gamma-tocotrienol, but C-40 side chain. formation was abolished Arabidopsis...
Endocrine disrupting compounds and in particular estrogenic substances have the ability to interact with hormone system of organisms. Among them are not only synthetic but also natural that potentially stress aquatic ecosystem. High human population densities such as around Pearl River Estuary (PRE) suspected exerting significant anthropogenic pressure onto coastal areas. In this study, estrogens well estrogen-like derived from plants fungi were investigated PRE at adjacent northern shelf...
Recent studies in aquatic environments have indicated that microbial methane production is not limited to strictly anoxic conditions and widespread the oxic water column. Based on recent investigations proposing linkage between turnover of methylphosphonate (MPn) oversaturation surface waters, we conducted an MPn/13C-MPn tracer approach combines liquid chromatography-mass spectrometry gas chromatography-stable isotope ratio mass assess concentrations MPn its contribution formation. In our...
Compounds such as estradiol and ethinylestradiol belong to contaminants of emerging concern, they can disrupt the endocrine system an organism with a hormonal system. The determination compounds is still challenging due required low detection quantification limits. Bioassays have proved be sensitive tools for investigating full potential all that elicit estrogenic response. In this study, surface water samples from different sampling sites seasons in Baltic Sea were analyzed activity Arxula...
Abstract Glyphosate (GLYP), the globally most important herbicide, may have effects in various compartments of environment such as soil and water. Although laboratory studies showed fast microbial degradation a low leaching potential, it is often detected environmental compartments, but pathways are unknown. Therefore, objective was to study GLYP transformations lysimeter field experiment over period one hydrological year using non-radioactive 13 C 2 - 15 N-GLYP labelling maize cultivation....
Glucosamine sulfate (GS) is known to stop the degenerative process of osteoarthritis. Because most GS formulation on market in oral form, an alternative such as a transdermal delivery system (TDS) necessary order increase patient compliance. As initial step develop TDS GS, physicochemical stability and permeation study rat skin were examined. Evaluation at different pHs showed compound be stable pH 5.0. The degradation rate constant 25°C was estimated 5.93 ×10− 6 hr− 1 (t90~ 2.03 years) 5...
Abstract A multiresidue method optimization was conducted using the design of experiment approach. Out 43 tested organophosphate compounds, 27 could be validated in surface water, yielding limits detection 0.6 to 2.5 ng L by dispersive liquid‐liquid micro‐extraction and subsequent liquid chromatography‐tandem quadrupole mass spectrometry (LC‐MSMS) detection. For sample preparation, factors sonication time, volume, binary extraction ratio, addition salt have been investigated Taguchi One...
Stable isotope labeling of pollutants is a valuable tool to investigate their environmental transport and degradation. For the globally most frequently used herbicide glyphosate, such studies have, so far, been hampered by absence an analytical standard for its labeled metabolite AMPA-15N, which formed during degradation all commercially available glyphosate isotopologues. Without standard, detection quantitation e.g. with LC-MS/MS, not possible. Therefore, synthetic pathway AMPA-15N from...