A5071132161- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Metal complexes synthesis and properties
- Ferrocene Chemistry and Applications
- Crystallography and molecular interactions
- Organometallic Complex Synthesis and Catalysis
- Click Chemistry and Applications
- Analytical chemistry methods development
- Lanthanide and Transition Metal Complexes
- RNA modifications and cancer
- Nanoparticle-Based Drug Delivery
- Advanced biosensing and bioanalysis techniques
- Dendrimers and Hyperbranched Polymers
- Microbial Community Ecology and Physiology
- Nanoplatforms for cancer theranostics
- Mass Spectrometry Techniques and Applications
- Cancer Treatment and Pharmacology
- Peptidase Inhibition and Analysis
- RNA and protein synthesis mechanisms
- Biotin and Related Studies
- Wastewater Treatment and Nitrogen Removal
- Fungal Plant Pathogen Control
- Metal-Catalyzed Oxygenation Mechanisms
- Microbial Fuel Cells and Bioremediation
- Slime Mold and Myxomycetes Research
University of Vienna
2012-2024
Institute of Inorganic Chemistry of the Slovak Academy of Sciences
2017-2024
Austrian Economics Center
2015-2017
Czech Academy of Sciences, Institute of Inorganic Chemistry
2017
The RAPTA pharmacophore was linked to beads identify its biomolecular targets in cancer cells.
NanoSIMS combined with fluorescence microscopy reveals subcellular structures in cancer cells where 15 N-labeled cisplatin is accumulated, implications for Pt–N bond integrity.
RuII(arene) anticancer compounds with maleimide functionality were prepared to allow selective interaction thiol-containing biomolecules and thereby enforcing the delivery of tumour.
A series of six novel bis(carboxylato)dichloridobis(ethylamine)platinum(IV) complexes was synthesized and characterized in detail by elemental analysis, FT-IR, ESI-MS, HPLC, multinuclear (1H, 13C, 15N, 195Pt) NMR spectroscopy one case X-ray diffraction. Cytotoxic properties the were evaluated four human tumor cell lines originating from ovarian carcinoma (CH1 SK-OV-3), colon (SW480) non-small lung cancer (A549) means MTT colorimetrical assay. In addition, their octanol/water partition...
The ruthenium-based anticancer agent BOLD-100/KP1339 has shown promising results in several vitro and vivo tumour models as well early clinical trials. However, its mode of action remains to be fully elucidated. Recent evidence identified stress induction the endoplasmic reticulum (ER) concomitant down-modulation HSPA5 (GRP78) key drug effects. By exploiting naturally formed adduct between BOLD-100 human serum albumin an immobilization strategy, we were able perform target-profiling...
Multicellular tumor spheroid models serve as an important three-dimensional in vitro cell model system they mimic the complex microenvironment and thus have contributed to valuable assays drug discovery studies. In this study, we present a state-of-the-art laser ablation-inductively coupled plasma mass spectrometry (LA-ICPMS) setup for high spatial resolution elemental imaging of multicellular spheroids approach account variations density. A low dispersion LA-ICPMS was employed, providing...
Pseudomonas syringae is a common plant pathogen, posing significant threats to the global crop production. By producing ice-nucleating proteins (INpro), encoded by inaZ gene, cells can inflict frost injuries plants, gaining access nutrient-rich tissue. Furthermore, P. impact cloud formation and interfere with atmospheric chemistry through their metabolic ice-nucleation activity. Both activity gene expression under conditions remain poorly understood, limiting our ability accurately predict...
Albumin-targeting of a maleimide-containing oxaliplatin-releasing platinum( iv ) prodrug results in tumor-specific drug delivery and activity as shown by LA-ICP-MS, isotope-labeling NanoSIMS cell culture vivo .
The synthesis and in vitro cytotoxicity of a series RuII(arene) complexes with carbohydrate-derived phosphite ligands various arene co-ligands is described. ligand has strong influence on the anticancer activity this compounds, which correlates fairly well cellular accumulation. most lipophilic compound bearing biphenyl moiety cyclohexylidene-protected carbohydrate cytotoxic unprecedented IC50 values for class three human cancer cell lines. This shows reactivity to DNA model nucleobase...
A NanoSIMS, LA-ICP-MS and TEM combinatorial approach was applied to analyze the distribution of platinum( iv ) prodrugs in murine tissue samples.
Abstract Microbial activity in drylands tends to be confined rare and short periods of rain. Rapid growth should key the maintenance ecosystem processes such narrow windows, if desiccation rehydration cause widespread cell death due osmotic stress. Here, simulating rain with 2 H O followed by single-cell NanoSIMS, we show that biocrust microbial communities Negev Desert are characterized limited productivity, median replication times 6 19 days restricted number allowing growth....
Guanidine is a chemically stable nitrogen compound that excreted in human urine and widely used manufacturing of plastics, as flame retardant component propellants, well known protein denaturant biochemistry
Abstract With the aim of systematically studying fundamental structure–activity relationships as a basis for development Ru II arene complexes (arene= p ‐cymene or biphenyl) bearing mono‐, bi‐, tridentate am(m)ine ligands anticancer agents, series ammine, ethylenediamine, and diethylenetriamine were prepared by different synthetic routes. Especially synthesis di‐, triammine was found to be highly dependent on reaction conditions, such stoichiometry, temperature, time. Hydrolysis...
The novel guanidine compounds trans-[Pt(NH2Me)2{NH=C(NHMe)NR}2](Cl)2 (R=NEt2 [7], NC5H10 [8]) (trans-7,8) were synthesized by the nucleophilic addition of methylamine to dialkylcyanamide ligands push-pull nitrile complexes trans-[PtCl2(RCN)2] (R=NEt2, NC5H10). In vitro cytotoxicity tests conducted for entire series complexes, i.e. trans-7,8, neutral cis- or trans-[PtCl2{NH=C(NH2)R}2] (cis-1-3 and trans-1-3) cationic trans-[Pt(NH3)2{NH=C(NH2)R}2](Cl)2 (cis-4-6 trans-4-6) (R=NMe2 [1,4], NEt2...
The reaction of 1,3-dihydroxyacetone oxime with diam(m)minediaquaplatinum(II) under basic conditions produced zwitterionic diam(m)mine(3-hydroxy-2-(oxidoimino)propan-1-olato-κ2N,O)platinum(II) complexes featuring the N,O-chelating ligand. Upon hydrochloric acid, it was possible to isolate either singly protonated species still exhibiting intact N,O-chelate or open-chain chlorido complex. All were characterized in detail multinuclear (1H, 13C, and 195Pt) NMR spectroscopy, ESI mass...
Abstract Ferrocenyl compounds show interesting antiproliferative properties. Consequently, ferrocene bearing moieties were prepared and coupled for the first time to anticancer platinum(IV) complexes. The compounds, featuring either one or two axially coordinated ferrocene‐containing ligands, fully characterized by ESI‐MS multinuclear ( 1 H, 13 C, 15 N, 195 Pt) one‐ two‐dimensional NMR spectroscopy. Their cytotoxicity was investigated in three human cancer cell lines deriving from ovarian...
The reaction of [Ru(CO)6Cl2], 1, with N[combining low line]3-methylbenzimidazole (MBI) and 5,6-dimethylbenzimidazole (DMBI) afforded two new complexes the general formula fac-[RuII(CO)3Cl2L], L = MBI (2) or DMBI (4). Crystals cis,trans-[RuII(CO)2Cl2(N[combining line]3-MBI)2], 3, were also obtained from mother liquor that produced 2. In presence water, dissociation Ru-N, Ru-Cl Ru-CO bonds occurred as a function time, water content pH. Density functional theory structure...
4-Phenylthiazole-based metalacycles were synthesized, characterized and examined for their anticancer potential. Preliminary mode of action studies to reveal the cellular target these complexes performed.
Novel platinum complexes of trans geometry [PtCl(2){(Z)-R(H)C═NOH}(2)] [R = Me (1), Et (3)] and [PtCl(2){(E)-R(H)C═NOH}{(Z)-R(H)C═NOH}] (2), (4)] as well the classic trans-[PtCl(2)(R(2)C═NOH)(2)] Me, Et] were reacted with an equivalent amount silver acetate in acetone solution at ambient temperature, resulting formation unprecedented head-to-tail-oriented oximato-bridged dimers [PtCl{μ-(Z)-R(H)C═NO}{(Z)-R(H)C═NOH}](2) (5), (7)], [PtCl{μ-(Z)-R(H)C═NO}{(E)-R(H)C═NOH}](2) (6), (8)],...
Oxaliplatin shows a superior clinical activity in colorectal cancer compared to cisplatin. Nevertheless, the knowledge about its cellular distribution and mechanisms responsible for different range of oxaliplatin-responsive tumors is far from complete. In this study, we combined highly sensitive element specific isotope selective imaging by nanometer-scale secondary ion mass spectrometry (NanoSIMS) with transmission electron microscopy investigate subcellular accumulation oxaliplatin three...
Abstract A series of 16 dinuclear thiopyridone‐based organometallics with excellent water solubility, increased stability and remarkable cytotoxicity were synthesized characterized. The complexes this work formed dimeric species featuring a double positive charge in polar protic solvents, accounting for their outstanding solubility aqueous solution. Most them displayed higher antiproliferative activity than parental thiomaltol complex, unexpected trends depending on the employed metal...
The reaction of<italic>cis</italic>-[PtCl<sub>2</sub>(Me<sub>2</sub>S̲O)<sub>2</sub>] with amidoximes RC(NH<sub>2</sub>)NOH results in selective generation of two types complexes,<italic>viz.</italic>open-chain and chelated, depending on the media.
Combining therapeutic with diagnostic agents (theranostics) can revolutionize the course of malignant diseases. Chemotherapy, hyperthermia, or radiation are used together methods such as magnetic resonance imaging (MRI). In contrast to conventional (CAs), which only enable non-specific visualization tissues and organs, theranostic probe offers targeted therapy simultaneously.Novel salinomycin (Sal)-based probes comprising two different paramagnetic metal ions, gadolinium(III) (Gd(III))...