A5029758467- Advanced Proteomics Techniques and Applications
- Lung Cancer Treatments and Mutations
- Microtubule and mitosis dynamics
- Cancer, Hypoxia, and Metabolism
- Neuroblastoma Research and Treatments
- Bioinformatics and Genomic Networks
- Cancer Genomics and Diagnostics
- Mass Spectrometry Techniques and Applications
- Gene Regulatory Network Analysis
- RNA modifications and cancer
- interferon and immune responses
- Metabolomics and Mass Spectrometry Studies
- RNA Research and Splicing
- Single-cell and spatial transcriptomics
- Chronic Lymphocytic Leukemia Research
- Gene expression and cancer classification
- Chronic Myeloid Leukemia Treatments
- Computational Drug Discovery Methods
- Protein Degradation and Inhibitors
- Radiomics and Machine Learning in Medical Imaging
- Cancer, Lipids, and Metabolism
- Ubiquitin and proteasome pathways
- Protein Structure and Dynamics
- Genomics and Chromatin Dynamics
- Monoclonal and Polyclonal Antibodies Research
Institut Regional du Cancer de Montpellier
2019-2025
Institut de Recherche en Cancérologie de Montpellier
2016-2025
Inserm
2016-2025
Université de Montpellier
2016-2025
Institut de Radiobiologie Cellulaire et Moléculaire
2020-2021
Austrian Academy of Sciences
2007-2016
CeMM Research Center for Molecular Medicine
2007-2016
Austrian Economics Center
2015
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2015
Moffitt Cancer Center
2012
Zeroing in on essential human genes More powerful genetic techniques are helping to define the list of required for life a cell. Two papers used CRISPR genome editing system and gene trap method haploid cells screen (see Perspective by Boone Andrews). Wang et al. 's analysis multiple cell lines indicates that it may be possible find tumor-specific dependencies particular genes. Blomen investigate phenomenon which nonessential fitness absence another gene. Hence, complexity rather than...
Type 1 diabetes is characterized by the destruction of pancreatic β cells, and generating new insulin-producing cells from other cell types a major aim regenerative medicine. One promising approach transdifferentiation developmentally related types, including glucagon-producing α cells. In genetic model, loss master regulatory transcription factor Arx sufficient to induce conversion functional β-like Here, we identify artemisinins as small molecules that functionally repress causing its...
Single-cell transcriptomics offers unprecedented opportunities to infer the ligand-receptor (LR) interactions underlying cellular networks. We introduce a new, curated LR database and novel regularized score perform such inferences. For first time, we try assess confidence in predicted show that our outperforms other scoring schemes while controlling false positives. SingleCellSignalR is implemented as an open-access R package accessible entry-level users available from...
The cell proliferation antigen Ki-67 is widely used in cancer histopathology, but estimations of expression levels are inconsistent and understanding its regulation limited. Here we show that cell-cycle underlies variable all situations analyzed, including nontransformed human cells, normal mouse intestinal epithelia adenomas, lines with or without drug treatments, breast colon cancers. In was a late marker entry; mRNA oscillated highest G2 while protein increased throughout the cycle,...
Abstract Mass spectrometry combined with database searching has become the preferred method for identifying proteins in proteomics projects. Proteins are digested by one or several enzymes to obtain peptides, which analyzed mass spectrometry. We introduce a new family of scoring schemes, named OLAV, aimed at peptides from their tandem spectra. OLAV schemes based on signal detection theory, and exploit information more extensively than previously existing schemes. also concept structural...
Dasatinib is a small-molecule kinase inhibitor used for the treatment of imatinib-resistant chronic myelogenous leukemia (CML). We have analyzed kinases targeted by dasatinib using an unbiased chemical proteomics approach to detect binding proteins directly from lysates CML cells. Besides Abl and Src kinases, we identified Tec Btk Tec, but not Itk, as major binders dasatinib. The activity was inhibited nanomolar concentrations in vitro cultured gatekeeper residue critical determinant...
Recognition of pathogens by the innate immune system requires proteins that detect conserved molecular patterns. Nucleic acids are recognized cytoplasmic sensors as well endosomal Toll-like receptors (TLRs). It has become evident TLRs require additional to be activated their respective ligands. In this study, we show CD14 (cluster differentiation 14) constitutively interacts with MyD88-dependent TLR7 and TLR9. was necessary for TLR7- TLR9-dependent induction proinflammatory cytokines in...
Abstract Necroptosis is a form of regulated necrotic cell death mediated by receptor-interacting serine/threonine-protein kinase 1 (RIPK1) and RIPK3. Necroptotic contributes to the pathophysiology several disorders involving tissue damage, including myocardial infarction, stroke ischemia-reperfusion injury. However, no inhibitors necroptosis are currently in clinical use. Here we performed phenotypic screen for small-molecule tumor necrosis factor-alpha (TNF -α )-induced Fas-associated...
The tyrosine kinase Bcr-Abl causes chronic myeloid leukemia and is the cognate target of inhibitors like imatinib. We have charted protein-protein interaction network by a 2-pronged approach. Using monoclonal antibody we first purified endogenous protein complexes from CML K562 cell line characterized set most tightly-associated interactors MS. Nine were subsequently subjected to tandem affinity purifications/MS analysis obtain molecular some hundred cellular proteins. resulting revealed...
Transport of RNAs to dendrites occurs in neuronal RNA granules, which allows local synthesis specific proteins at active synapses on demand, thereby contributing learning and memory. To gain insight into the machinery controlling dendritic mRNA localization translation, we established a stringent protocol biochemically purify granules from rat brain. Here, identified set interactors for two RNA-binding that are known components Barentsz Staufen2. First, much more heterogeneous than...
The RAPTA pharmacophore was linked to beads identify its biomolecular targets in cancer cells.
Primary immunodeficiency disorders enable identification of genes with crucial roles in the human immune system. Here we study patients suffering from recurrent bacterial, viral and Cryptosporidium infections, identify a biallelic mutation MAP3K14 gene encoding NIK (NF-κB-inducing kinase). Loss kinase activity mutant NIK, predicted by silico analysis confirmed functional assays, leads to defective activation both canonical non-canonical NF-κB signalling. Patients mutated exhibit B-cell...
Quantitative comparison of the protein content biological samples is a fundamental tool research. The TMT and iTRAQ isobaric labeling technologies allow 2, 4, 6, or 8 in one mass spectrometric analysis. Sound statistical models that scale with most advanced spectrometry (MS) instruments are essential for their efficient use. Through application robust methods, we developed capture variability from individual spectra to samples. Classical experimental designs distinct sample each channel as...