A5009288774- Cancer Cells and Metastasis
- Epigenetics and DNA Methylation
- Helicobacter pylori-related gastroenterology studies
- Digestive system and related health
- Cancer-related molecular mechanisms research
- Cancer Genomics and Diagnostics
- Biochemical Analysis and Sensing Techniques
- Cancer-related gene regulation
- Wnt/β-catenin signaling in development and cancer
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Single-cell and spatial transcriptomics
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Gene Regulatory Network Analysis
- Neonatal Respiratory Health Research
- Clinical Nutrition and Gastroenterology
- Barrier Structure and Function Studies
- Chromosomal and Genetic Variations
- IL-33, ST2, and ILC Pathways
- Animal Genetics and Reproduction
- Diet, Metabolism, and Disease
- Genetic factors in colorectal cancer
- Radiopharmaceutical Chemistry and Applications
- Gene expression and cancer classification
- Monoclonal and Polyclonal Antibodies Research
Institut de Génomique Fonctionnelle
2012-2025
Inserm
2014-2025
Centre National de la Recherche Scientifique
2012-2025
Université de Montpellier
2012-2025
La Ligue Contre le Cancer
2021-2022
Centre National pour la Recherche Scientifique et Technique (CNRST)
2016
Genomics (United Kingdom)
2011
Hôtel-Dieu de France
2006
Institut de Génétique Humaine
2004-2005
Centre de Recherche en Biologie cellulaire de Montpellier
1995-1999
The signal transducer and activator of transcription–3 (Stat3) protein is activated by the interleukin 6 (IL-6) family cytokines, epidermal growth factor, leptin. A named PIAS3 (protein inhibitor STAT) that binds to Stat3 was isolated characterized. association with in vivo only observed cells stimulated ligands cause activation Stat3. blocked DNA-binding activity inhibited Stat3-mediated gene activation. Although Stat1 also phosphorylated response IL-6, did not interact or affect its...
TCF and SOX proteins belong to the high mobility group box transcription factor family. Whereas TCFs, transcriptional effectors of Wnt pathway, have been widely implicated in development, homeostasis disease intestine epithelium, little is known about function this tissue. Here, we identified SOX9 a expression screening mouse fetal intestine. We report that protein expressed intestinal epithelium pattern characteristic targets. provide vitro vivo evidence bipartite β-catenin/TCF4 factor,...
The HMG-box transcription factor Sox9 is expressed in the intestinal epithelium, specifically, stem/progenitor cells and Paneth cells. expression requires an active beta-catenin-Tcf complex, transcriptional effector of Wnt pathway. This pathway critical for numerous aspects epithelium physiopathology, but processes that specify cell response to such multipotential signals still remain be identified. We inactivated gene analyze its physiological function. inactivation affected differentiation...
The unique morphology of tuft cells was first revealed by electron microscopy analyses in several endoderm-derived epithelia. Here, we explore the relationship these with other cell types intestinal epithelium and describe marker signature allowing their unambiguous identification. We demonstrate that although mature express DCLK1, a putative quiescent stem cells, they are post-mitotic, short lived, derive from Lgr5-expressing epithelial found mouse human tumors. show whereas ATOH1/MATH1...
The cell proliferation antigen Ki-67 is widely used in cancer histopathology, but estimations of expression levels are inconsistent and understanding its regulation limited. Here we show that cell-cycle underlies variable all situations analyzed, including nontransformed human cells, normal mouse intestinal epithelia adenomas, lines with or without drug treatments, breast colon cancers. In was a late marker entry; mRNA oscillated highest G2 while protein increased throughout the cycle,...
Antigen Ki-67 is a nuclear protein expressed in proliferating mammalian cells. It widely used cancer histopathology but its functions remain unclear. Here, we show that controls heterochromatin organisation. Altering expression levels did not significantly affect cell proliferation vivo. mutant mice developed normally and cells lacking proliferated efficiently. Conversely, upregulation of differentiated tissues prevent cycle arrest. interactors included proteins involved nucleolar processes...
Upon parasitic helminth infection, activated intestinal tuft cells secrete interleukin-25 (IL-25), which initiates a type 2 immune response during lamina propria innate lymphoid (ILC2s) produce IL-13. This causes epithelial remodeling, including cell hyperplasia, the function of is unknown. We identified cholinergic effector cells, are only that expressed choline acetyltransferase (ChAT). During parasite mice with epithelial-specific deletion ChAT had increased worm burden, fitness, and...
T cell factor / lymphocyte enhancer (Tcf Lef) transcription factors complex with the transcriptional co-activator β-catenin to transduce Wnt signals in a variety of developmental systems. The prototypic family member Tcf-1 is highly expressed lineage cells. Tcf1– – mice are defective cycling early thymocyte stages. Here, we show that interaction required for full development. This may be established by mediated Wnt1 and Wnt4, leading increased Tcf-dependent activity thymocytes, as...
Gut microbiota imbalance (dysbiosis) is increasingly associated with pathological conditions, both within and outside the gastrointestinal tract. Intestinal Paneth cells are considered to be guardians of gut microbiota, but events linking cell dysfunction dysbiosis remain unclear. We report a three-step mechanism for initiation. Initial alterations in cells, as frequently observed obese inflammatorybowel diseases patients, cause mild remodeling amplification succinate-producing species....
Abstract Tight junctions have recently emerged as essential signaling regulators of proliferation and differentiation in epithelial tissues. Here, we aimed to identify the factors regulating claudin-7 expression colon, analyzed consequences overexpression colorectal carcinoma (CRC). In healthy human colonic crypts, was found be low stem/progenitor cell compartment, where Tcf-4 activity is high, but strong differentiated postmitotic cells, inactive. contrast, overexpressed areas with high...
Helminth parasites are adept manipulators of the immune system, using multiple strategies to evade host type 2 response. In intestinal niche, epithelium is crucial for initiating immunity via tuft cells, which together with goblet cells expand dramatically in response cytokines IL-4 and IL-13. However, it not known whether helminths modulate these epithelial cell populations. vitro, small organoids, we found that excretory/secretory products (HpES) from Heligmosomoides polygyrus blocked...
The idea that stem cells of adult tissues with high turnover are protected from DNA replication-induced mutations by maintaining the same 'immortal' template strands together through successive divisions has been tested in several tissues. In epithelium small intestine, provided evidence was based on assumption located above Paneth cells. results genetic lineage-tracing experiments point instead to crypt base columnar intercalated between as bona fide Here we show these segregate most, if...
Macrophage migration inhibitory factor (MIF) is a key innate immune mediator with chemokine- and cytokine-like properties in the inflammatory pathway. While its actions on macrophages are well-studied, effects other cell types less understood. Here we report that MIF required for expansion of intestinal tuft cells during infection helminth Nippostrongylus brasiliensis. MIF-deficient mice show defective responses following infection, lacking epithelial hyperplasia or upregulation goblet...
Osteoblasts and their precursors respond to specific cytokines, growth factors, hormones. One facet of this response includes the secretion additional some which are part circuitry involved in regulation osteoblast osteoclast function. Therefore, understanding cytokines able activate osteoblastic cells consequences that activation central normal pathologic bone remodeling. Oncostatin M (OSM) is a glycoprotein belonging new subfamily related by sequence structural homology use signal...