A5016678467- Mitochondrial Function and Pathology
- Retinal Development and Disorders
- Hearing, Cochlea, Tinnitus, Genetics
- Retinal Diseases and Treatments
- Escherichia coli research studies
- Brucella: diagnosis, epidemiology, treatment
- Endoplasmic Reticulum Stress and Disease
- ATP Synthase and ATPases Research
- Mosquito-borne diseases and control
- Vibrio bacteria research studies
- Neuroscience and Neuropharmacology Research
- Fungal and yeast genetics research
- RNA regulation and disease
- Lipid metabolism and disorders
- Retinopathy of Prematurity Studies
- Cellular transport and secretion
- Ubiquitin and proteasome pathways
- Circadian rhythm and melatonin
- RNA Research and Splicing
- Ion channel regulation and function
- Ferroptosis and cancer prognosis
- Plant Reproductive Biology
- Salmonella and Campylobacter epidemiology
- Plant Gene Expression Analysis
- Legionella and Acanthamoeba research
Institute for Neurosciences of Montpellier
2016-2025
Inserm
2014-2025
Université de Montpellier
2014-2025
Hôpital Saint Eloi
2005-2025
Neuroscience Institute
2022
Centre Hospitalier Universitaire de Montpellier
2016
Institut Universitaire de Recherche Clinique
2007-2016
Université Toulouse III - Paul Sabatier
2003
Ministère de l’Enseignement Supérieur et de la Recherche
2002
University of Oxford
1996-1997
Analysis of a Tn blaM mutant Brucella suis 1330, identified as being unable to multiply in Hela cells, allowed us identify 11 860 bp region the B. genome encoding type IV secretion system, homologous VirB system Agrobacterium tumefaciens and Ptl Bordetella pertussis . DNA sequence revealed 12 open reading frames (ORFs) homologues proteins present pTi plasmid with similar genetic organization, twelfth ORF putative lipoprotein, protein involved mating pair formation during bacterial...
A type IV secretion system similar to the VirB of phytopathogen Agrobacterium tumefaciens is essential for intracellular survival and multiplication mammalian pathogen Brucella . Reverse transcriptase–PCR showed that 12 genes encoding suis form an operon. Semiquantitative measurements virB mRNA levels by slot blotting transcription operon, but not flanking genes, regulated environmental factors in vitro Flow cytometry used measure green fluorescent protein expression from promoter confirmed...
The Ca 2+ sensor NCS1 promotes ER-mitochondria contact and transfer between these organelles.
Gut microbiota imbalance (dysbiosis) is increasingly associated with pathological conditions, both within and outside the gastrointestinal tract. Intestinal Paneth cells are considered to be guardians of gut microbiota, but events linking cell dysfunction dysbiosis remain unclear. We report a three-step mechanism for initiation. Initial alterations in cells, as frequently observed obese inflammatorybowel diseases patients, cause mild remodeling amplification succinate-producing species....
ABSTRACT Bacteria of the genus Brucella are facultative intracellular pathogens which have developed capacity to survive and multiply in professional nonprofessional phagocytes. The genetic basis this aspect virulence is still poorly understood. To identify new factors, we adapted signature-tagged transposon mutagenesis, has been used essentially animal models, an vitro human macrophage infection model. A library 1,152 suis 1330 tagged mini-Tn 5 Km2 mutants, 12 pools, was screened for...
Dominant optic atrophy is a rare inherited nerve degeneration caused by mutations in the mitochondrial fusion gene OPA1. Recently, clinical spectrum of dominant has been extended to frequent syndromic forms, exhibiting various degrees neurological and muscle impairments frequently found diseases. Although characterized specific loss retinal ganglion cells, pathophysiology still poorly understood. We generated an Opa1 mouse model carrying recurrent Opa1(delTTAG) mutation, which 30% all...
Mutations in genes encoding components of the mitochondrial DNA (mtDNA) replication machinery cause mtDNA depletion syndromes (MDSs), which associate ocular features with severe neurological syndromes. Here, we identified heterozygous missense mutations single-strand binding protein 1 (SSBP1) 5 unrelated families, leading to R38Q and R107Q amino acid changes single-stranded DNA-binding protein, a crucial involved replication. All affected individuals presented optic atrophy, associated...
SARS-CoV-2 is an enveloped virus responsible for the Coronavirus Disease 2019 (COVID-19) pandemic. Here, single viruses were analyzed by atomic force microscopy (AFM) operating directly in a level 3 biosafety (BSL3) facility, which appeared as fast and powerful method to assess at nanoscale 3D infectious morphology its native conformation, or upon inactivation treatments. AFM imaging reveals structurally intact inactivated low concentration of formaldehyde treatment. This protocol combining...
Dominant variants in
Retroviral assembly is driven by Gag, and nascent viral particles escape cells recruiting the machinery that forms intralumenal vesicles of multivesicular bodies. In this study, we show clathrin adaptor complex AP-1 involved in retroviral release. The absence AP-1μ obtained genetic knock-out or RNA interference reduces budding murine leukemia virus (MLV) HIV-1, leading to a delay propagation cell culture. contrast, overexpression enhances release HIV-1 Gag. We facilitates through direct...
Gruels tailored to school-age children and made of soy milk rice flour with or without total dietary fiber from passion fruit by-product were fermented by amylolytic lactic acid bacteria strains (Lactobacillus fermentum Ogi E1 Lactobacillus plantarum A6), commercial probiotic acidophilus L10, casei L26 Bifidobacterium animalis subsp. lactis B94) co-cultures one strain. The influence ingredient composition bacterial cultures on kinetics acidification, α-amylase activity the bacteria, apparent...
The reproductive parasites Wolbachia are the most common endosymbionts on earth, present in a plethora of arthropod species. They have been introduced into mosquitos to successfully prevent spread vector-borne diseases, yet strategies host cell subversion underlying their obligate intracellular lifestyle remain be explored depth order gain insights mechanisms pathogen-blocking. Like some other bacteria, reside host-derived vacuole replicate and escape immune surveillance. Using here...
Apical extracellular matrices (aECMs) form a physical barrier to the environment. In Caenorhabditis elegans, epidermal aECM, cuticle, is composed mainly of different types collagen, associated in circumferential ridges separated by furrows. Here, we show that mutants lacking furrows, normal intimate connection between epidermis and cuticle lost, specifically at lateral epidermis, where, contrast dorsal ventral there are no hemidesmosomes. At ultrastructural level, profound alteration...
Bacteria from the Brucella genus are able to survive and proliferate within macrophages. Because they phylogenetically closely related macrophages, myeloid dendritic cells (DCs) constitute potential targets for bacteria. Here we report that DCs display a great susceptibility infection. Therefore, might serve as reservoir be important development of bacteria their host.
Upon maturation in the bone marrow, polyploid megakaryocytes elongate very long and thin cytoplasmic branches called proplatelets. Proplatelets enter sinusoids blood vessels which platelets are ultimately released. Microtubule dynamics, bundling, sliding, coiling, drive these dramatic morphological changes whose regulation remains poorly understood. properties defined by tubulin isotype composition post-translational modification patterns. It unknown whether microtubule modifications occur...
Wolfram syndrome (WS) is a rare neurodegenerative disorder encompassing diabetes mellitus, insipidus, optic atrophy, hearing loss (HL) as well neurological disorders. None of the animal models pathology are presenting with an early onset HL, impeding understanding role Wolframin (WFS1), protein responsible for WS, in auditory pathway. We generated knock-in mouse, Wfs1E864K line, human mutation leading to severe deafness affected individuals. The homozygous mice showed profound post-natal HL...
Plasmodium malariae is the third most prevalent human malaria parasite species and contributes significantly to morbidity. Nevertheless, our comprehension of this parasite’s biology remains limited, primarily due its frequent co-infections with other lack a continuous in vitro culture system. To effectively combat eliminate overlooked parasite, it imperative acquire better understanding species. In study, we embarked on an investigation P. , including exploring clinical disease...
Tubulin detyrosination has been implicated in various human disorders and is important for regulating microtubule dynamics. While most organisms this modification restricted to α-tubulin, trypanosomatid parasites, it occurs on both α- β-tubulin. Here, we show that Leishmania , a single vasohibin (LmVASH) enzyme responsible differential kinetics of β-tubulin detyrosination. LmVASH knockout which are completely devoid detyrosination, decreased levels glutamylation exhibit strongly diminished...
Abstract Localization of mRNAs to neuronal terminals, coupled local translation, has emerged as a prevalent mechanism controlling the synaptic proteome. However, physiological regulation and function this process in context mature vivo memory circuits remained unclear. Here, we combined synaptosome RNA profiling with whole brain high-resolution imaging uncover different localization patterns axons Drosophila Mushroom Body neurons, some exhibiting regionalized, input-dependent, recruitment...
The mammalian vestibular system has two types of sensory receptor hair cells (HCs), each with different neurotransmission mechanisms. Type II HCs use ribbon synapses to transmit neurotransmitters like glutamate afferent neurons. On the other hand, type I are nearly engulfed by a calyceal ending and also form synapses. These regulate activity through non-quantal transmission (NQT), which is faster than classic neurotransmitter release may play key role in stabilizing vision balance during...