A5065637948- Microtubule and mitosis dynamics
- Ubiquitin and proteasome pathways
- Cardiomyopathy and Myosin Studies
- Muscle Physiology and Disorders
- Lipid metabolism and disorders
- Cancer, Hypoxia, and Metabolism
- Cellular transport and secretion
- RNA Research and Splicing
- Cellular Mechanics and Interactions
- Cardiovascular Effects of Exercise
- Epigenetics and DNA Methylation
- Protist diversity and phylogeny
- Force Microscopy Techniques and Applications
- Platelet Disorders and Treatments
- Cancer-related Molecular Pathways
- Sphingolipid Metabolism and Signaling
- Research on Leishmaniasis Studies
- Genetics and Neurodevelopmental Disorders
- Hereditary Neurological Disorders
- Neuroscience and Neuropharmacology Research
- Biotin and Related Studies
- Renal Diseases and Glomerulopathies
- Enzyme Structure and Function
- Trypanosoma species research and implications
- Glycosylation and Glycoproteins Research
Centre de Recherche en Biologie cellulaire de Montpellier
2002-2025
Centre National de la Recherche Scientifique
2007-2025
Université de Montpellier
2002-2025
University of Georgia
2005
Sine Institute
2005
Université de Strasbourg
2005
Sorbonne Université
2005
Max Planck Institute for Medical Research
1999
Posttranslational glutamylation of tubulin is present on selected subsets microtubules in cells. Although the modification expected to contribute spatial and temporal organization cytoskeleton, hardly anything known about its functional relevance. Here we demonstrate that glutamylation, particular generation long glutamate side chains, promotes severing microtubules. In human cells, chains induces spastin-dependent microtubule disassembly and, consistently, only modified by are efficiently...
Tubulin carboxypeptidase identity revealed Enzymes of the α-tubulin detyrosination/tyrosination cycle create landmarks on microtubules that are essential for their multiple cellular functions and altered in disease. carboxypeptidases (TCPs) responsible detyrosination have remained elusive 40 years (see Perspective by Akhmanova Maiato). Aillaud et al. identified vasohibins as enzymes perform TCP function found small interacting partner SBVP was activity. Vasohibin/SVBP complexes were involved...
Polyglutamylation of tubulin has been implicated in several functions microtubules, but the identification responsible enzyme(s) challenging. We found that neuronal polyglutamylase is a protein complex containing tyrosine ligase-like (TTLL) protein, TTLL1. TTLL1 member large family proteins with TTL homology domain, whose members could catalyze ligations diverse amino acids to tubulins or other substrates. In model protist Tetrahymena thermophila, two conserved types polyglutamylases were...
The microtubule cytoskeleton is crucial for the internal organization of eukaryotic cells. Several microtubule-associated proteins link microtubules to subcellular structures. A subclass these proteins, plus end–binding (+TIPs), selectively binds growing ends microtubules. Here, we reconstitute a vertebrate end tracking system composed most prominent +TIPs, end-binding protein 1 (EB1) and CLIP-170, in vitro dissect their end-tracking mechanism. We find that EB1 autonomously recognizes...
Synaptic plasticity, the ability of synapses to change in strength, requires alterations synaptic molecule compositions over time, and undergo selective modifications on stimulation. Molecular motors operate sorting/transport neuronal proteins; however, targeting mechanisms that guide direct cargo delivery remain elusive. We addressed impact transmission regulation intracellular microtubule (MT)-based transport. show increased activity, as induced through GlyR activity blockade, facilitates...
Polyglutamylation is a post-translational modification that generates lateral acidic side chains on proteins by sequential addition of glutamate amino acids. This was first discovered tubulins, and it important for several microtubule functions. Besides only the nucleosome assembly NAP1 NAP2 have been shown to be polyglutamylated. Here, using proteomic approach, we identify large number putative substrates polyglutamylation in HeLa cells. By analyzing selection these substrates, show them...
Tubulin undergoes glutamylation, a conserved posttranslational modification of poorly understood function. We show here that in the ciliate Tetrahymena, most microtubule arrays contain glutamylated tubulin. However, length polyglutamyl side chain is spatially regulated, with longest chains present on ciliary and basal body microtubules. focused our efforts function glutamylation alpha-tubulin subunit. By site-directed mutagenesis, we all six glutamates C-terminal tail domain provide...
Abstract HSET is a mitotic kinesin essential for centrosome clustering in cells harboring supernumerary centrosomes. Previous work cellulo revealed physical and functional interaction between intraflagellar transport proteins to control efficient extra subsequent cancer cell proliferation. However, which IFT directly bind whether regulate activity remains unknown. Using reconstituted vitro system combining purified coupled TIRF microscopy approaches, we identified minimal subcomplex made of...
Upon maturation in the bone marrow, polyploid megakaryocytes elongate very long and thin cytoplasmic branches called proplatelets. Proplatelets enter sinusoids blood vessels which platelets are ultimately released. Microtubule dynamics, bundling, sliding, coiling, drive these dramatic morphological changes whose regulation remains poorly understood. properties defined by tubulin isotype composition post-translational modification patterns. It unknown whether microtubule modifications occur...
Summary The Lmj F01.0030 gene of Leishmania major Friedlin, annotated as ‘MCAK‐like’, was confirmed a kinesin with an internally located motor domain and termed LmjKIN13‐1 . Both the native form protein green fluorescent (GFP)‐fused recombinant version were shown to be exclusively intranuclear, and, more specifically, localize spindle poles. Cell cycle‐dependent regulation levels demonstrated using synchronized cells: KIN13‐1 highly abundant in G2+M phase present at very low after mitosis....
Tubulin glutamylation is a reversible posttranslational modification that accumulates on stable microtubules (MTs). While abnormally high levels of this lead to number disorders such as male sterility, retinal degeneration, and neurodegeneration, very little known about the molecular mechanisms underlying regulation glutamylase activity. Here, we found CSAP forms complex with TTLL5, demonstrate two proteins regulate their reciprocal abundance. Moreover, show increases TTLL5-mediated identify...
The role of the interaction between actin and secondary binding site myosin (segment 565-579 rabbit skeletal muscle myosin, referred to as loop 3 in this work) has been studied with proteolytically generated smooth subfragment 1 recombinant Dictyostelium discoideum II motor domain constructs. Carbodiimide-induced cross-linking filamentous took place only not those or D. II. Chimeric constructs containing either human nonmuscle were generated. Significant region was obtained chimera both...
// Mohamed Jemaà 1, 2 , Samer Abdallah Gwendaline Lledo Gaelle Perrot 3 Tom Lesluyes Catherine Teyssier 4 Pierre Roux Juliette van Dijk Frederic Chibon 3, 5 Ariane Abrieu Nathalie Morin 1 Universités de Montpellier, 34293 France CRBM, CNRS, UMR 5237, INSERM U1218, Bergonié Cancer Institute, F-33076 Bordeaux, U1194 INSERM, IRCM, 34298 Department of Pathology, Correspondence to: Ariane, email: ariane.abrieu@crbm.cnrs.fr Nathalie, nathalie.morin@crbm.cnrs.fr Keywords: sarcoma, CINSARC, mitosis,...
Tubulin post-translational modifications regulate microtubule properties and functions. Mitotic spindle microtubules are highly modified. While tubulin detyrosination promotes proper mitotic progression by recruiting specific microtubule-associated proteins motors, acetylation that occurs on subsets during mitosis is less well understood. Here, we show siRNA-mediated depletion of the acetyltransferase ATAT1 in epithelial cells leads to a prolonged prometaphase arrest formation monopolar...
Microtubules (MTs) play crucial roles during neuronal life. They are formed by heterodimers of alpha and beta-tubulins, which subjected to several post-translational modifications (PTMs). Amongst them, glutamylation consists in the reversible addition a variable number glutamate residues C-terminal tails tubulins. Glutamylation is most abundant MT PTM mammalian adult brain, suggesting that it plays an important role nervous system (NS). Here, we show previously uncharacterized CG31108 gene...
The interaction between skeletal myosin subfragment 1 (S1) and filamentous actin was examined at various intermediate states of the actomyosin ATPase cycle by chemical cross-linking experiments. Reaction actin−S1 complex with 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide N-hydroxysuccinimide generated products molecular masses 165 175 kDa, in which S1 loops residues 626−647 567−578 were cross-linked independently to N-terminal segment 1−12 one monomer, 265 two bound N termini adjacent...
Changes in the actin–myosin interface are thought to play an important role microfilament‐linked cellular movements. In this study, we compared actin binding properties of motor domain Dictyostelium discoideum (M765) and rabbit skeletal muscle myosin subfragment‐1 (S1). The resembles S1(A2) (S1 carrying A2 light chain) its interaction with G‐actin. Similar S1(A2), none constructs induced G‐actin polymerization. affinity monomeric (G‐actin) was 20‐fold lower for M765 than but increasing...
Mature megakaryocytes extend long processes called proplatelets from which platelets are released in the blood stream. The Rho GTPases Cdc42 and Rac as well their downstream target, p21-activated kinase 2 (PAK2), have been demonstrated to be important for platelet formation. Here we address role, during formation, of PAK1, another target GTPases. PAK1 decorates bundled microtubules (MTs) megakaryocyte proplatelets. Using a validated cell model recapitulates proplatelet elongation release,...