A5101513803- Probiotics and Fermented Foods
- Genetic diversity and population structure
- Microbial Metabolites in Food Biotechnology
- Gut microbiota and health
- Genetic and Kidney Cyst Diseases
- Microtubule and mitosis dynamics
- Microbial infections and disease research
- Retinal Development and Disorders
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Pancreatic function and diabetes
- Chromosomal and Genetic Variations
- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- Genetic Mapping and Diversity in Plants and Animals
- Hearing, Cochlea, Tinnitus, Genetics
- Molecular Biology Techniques and Applications
- Epigenetics and DNA Methylation
- Diabetes and associated disorders
- Cellular Mechanics and Interactions
- Cellular transport and secretion
- Inflammasome and immune disorders
- Identification and Quantification in Food
- Amino Acid Enzymes and Metabolism
- Retinoids in leukemia and cellular processes
Institut Pasteur
2015-2022
Centre National de la Recherche Scientifique
2004-2017
Institut Curie
2012-2017
Université Paris Sciences et Lettres
2013-2017
Université Paris-Saclay
2017
Université Paris-Sud
2017
Sorbonne Université
2016
Inserm
2013-2016
AB-Biotics (Spain)
2011-2013
Université de Montpellier
2004-2010
Microtubules are subject to a variety of posttranslational modifications that potentially regulate cytoskeletal functions. Two modifications, glutamylation and glycylation, highly enriched in the axonemes most eukaryotes, might therefore play particularly important roles cilia flagella. Here we systematically analyze dynamics glycylation developing mouse ependymal expression corresponding enzymes brain. By screening TTLL family for specific functions cilia, demonstrate glycylating TTLL3...
The precise architecture of hair bundles, the arrays mechanosensitive microvilli-like stereocilia crowning auditory cells, is essential to hearing. Myosin IIIa, defective in late-onset deafness form DFNB30, has been proposed transport espin-1 tips stereocilia, thereby promoting their elongation. We show that Myo3a−/−Myo3b−/− mice lacking myosin IIIa and IIIb are profoundly deaf, whereas Myo3a-cKO Myo3b−/− losing postnatally have normal cochlear bundles display robust mechanoelectrical...
ABSTRACT Tubulin is subject to a wide variety of posttranslational modifications, which, as part the tubulin code, are involved in regulation microtubule functions. Glycylation has so far predominantly been found motile cilia and flagella, absence this modification leads ciliary disassembly. Here, we demonstrate that correct functioning connecting photoreceptors, which non-motile sensory cilia, also dependent on glycylation. In contrast many other tissues, only one glycylase, TTLL3,...
The effectiveness of influenza vaccination in preventing illness is lower the elderly; this why ability Lactobacillus plantarum CECT 7315/7316 to stimulate response elderly was evaluated.A randomized, double-blind, placebo-controlled human trial including 60 institutionalized volunteers aged 65-85 years performed. All were vaccinated with a trivalent vaccine (A/Wisconsin/67/2005 NYMC X-161B (H3N2), A/Solomon Islands/3/2006 (H1N1) and B/Malaysia/2506/2004) for Spanish campaign 2006/2007....
Abstract BACKGROUND Hypercholesterolaemia plays a key role in the development and progression of coronary artery disease. There are numerous drugs available to treat this condition but they often expensive can have unwanted side effects. Therefore, screening lactic acid bacteria select candidate probiotic strains reduce cholesterol levels was carried out. Three Lactobacillus plantarum (CECT 7527, 7528 7529) were selected as potential probiotics after conducting several vitro tests for...
Background Ancestral reconstructions of mammalian genomes have revealed that evolutionary breakpoint regions are clustered in more prone to break and reorganize. What is still unclear biologists whether these physically unstable due solely sequence composition and/or genome organization, or do they represent genomic areas where the selection against breakpoints minimal. Methodology Principal Findings Here we present a comprehensive study distribution tandem repeats great apes. We analyzed...
Onconase is an RNase with a very specific property because it selectively toxic to transformed cells. This toxin thought recognize cell surface receptors, and the protection conferred by metabolic poisons against toxicity indicated that this relies on endocytic uptake kill Nevertheless, its internalization pathway has yet be unraveled. We show here enters cells using AP-2/clathrin-mediated endocytosis. It then routed, together transferrin, receptor recycling compartment. Increasing...
Not all lactic acid bacteria possess the ability to confer health benefits for host. Thus, it becomes necessary screen and characterize numerous strains obtain ideal probiotics. Here, two Lactobacillus plantarum (CECT 7315 CECT 7316) were isolated characterized.In vitro in vivo tests carried out demonstrating abilities as probiotics of 7315/CECT 7316 Lact. strains. Both showed high survive at gastro-intestinal tract conditions adhere intestinal epithelial cells, well great inhibitory...
Some members of the ribonuclease superfamily, such as Onconase, are cytotoxic to cancer cells. This is not case for human pancreatic ribonuclease. lack cytotoxicity probably a result inhibition exerted by cytosolic inhibitor once protein has reached cytosol. Until now, all variants have been described being resistant inhibitor. Here, we report on characterization variant which an Arg triplet introduced onto one its surface-exposed loops. Despite sensitivity inhibitor, this variant, called...
The Pasteurella multocida znuACB genes encoding a high-affinity zinc-uptake system have been identified and cloned. In contrast to what happens in Escherichia coli, znuA is not physically linked znuCB. Through lacZ transcriptional fusions it has demonstrated that zinc negatively regulates both znuCB operons. Nevertheless, contrary determined so far for all other bacterial systems known, P. are under control of the zur gene, which absent this species, but rather its iron-uptake regulator...
Abstract Backgound The RPS4 gene codifies for ribosomal protein S4, a very well-conserved present in all kingdoms. In primates, is codified by two functional genes located on both sex chromosomes: the RPS4X and RPS4Y genes. humans, duplicated Y chromosome therefore carries third paralog: RPS4Y2 , which presents testis-specific expression pattern. Results DNA sequence analysis of intronic cDNA regions from species covering entire primate phylogeny showed that duplication event leading to...
The exbB, exbD and tonB genes of the Pasteurella multocida animal pathogen have been cloned by complementation an Escherichia coli mutant. Despite these three being physically linked, RT-PCR analysis, lacZ transcriptional fusions construction insertional mutants demonstrated that they do not constitute operon, but rather are transcribed independently from each other. Furthermore, expression is under iron control as revealed Fur titration assay analysis. Moreover, necessary for virulence P....
Onconase, a member of the ribonuclease superfamily, is potent cytotoxic agent that undergoing phase II/III human clinical trials as an antitumor drug. Native onconase from Rana pipiens and its amphibian homologs have N-terminal pyroglutamyl residue essential for obtaining fully active enzymes with their full potential cytotoxins. When expressed cytosolically in bacteria, Onconase isolated additional methionyl (Met1) glutaminyl instead at position 1 N-terminus consequently inactivated. The...
Abstract Background The popularity of microsatellites has greatly increased in the last decade on account their many applications. However, little is currently understood about factors that influence genesis and distribution among within species genomes. In this work, we analyzed carnivore microsatellite clones from GenBank to study association with interspersed repeats elucidate role latter distribution. Results We constructed a comprehensive database comprising 1236 GenBank. Thirty-three...
Abstract Objective : Interleukin (IL)‐18 has been associated with obesity and insulin resistance, both risk factors for the development of liver disease, but role IL‐18 in disease resistance is presently unknown. We hypothesized that circulating would be related to serum concentrations chemistry tests (LCTs) apparently healthy subjects wished study whether this correlation was dependent on sensitivity ( S I ). Research Methods Procedures One hundred six white men consecutively enrolled a...
Enzyme stability can be an important parameter in the design of recombinant toxins because unstable proteins are often degraded before they reach their cellular target. There is great interest human pancreatic ribonuclease variants that could cytotoxic against tumoral cells. To this end, some residues protein need to substituted, but may result a loss stability. Previous papers have reported production N- and C-terminal with increased thermal Here, we investigated contribution different...
ESR Endangered Species Research Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 9:133-142 (2009) - DOI: https://doi.org/10.3354/esr00191 Contribution Theme Section 'Primate conservation: measuring and mitigating trade in primates' First generation microarray-system for identification of primate species subject bushmeat A.-C. Rönn1, O. Andrés2, F. López-Giráldez2,3, C. Johnsson-Glans1, E. J....