A5023084463- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- DNA and Nucleic Acid Chemistry
- Aquaculture disease management and microbiota
- RNA modifications and cancer
- Protein Structure and Dynamics
- Vibrio bacteria research studies
- Enzyme Production and Characterization
- CRISPR and Genetic Engineering
- Enzyme Structure and Function
- Peptidase Inhibition and Analysis
- Cancer-related Molecular Pathways
- Biofuel production and bioconversion
- Cancer therapeutics and mechanisms
- Bacteriophages and microbial interactions
- RNA Research and Splicing
- Glutathione Transferases and Polymorphisms
- Advanced biosensing and bioanalysis techniques
- Ubiquitin and proteasome pathways
- RNA Interference and Gene Delivery
- Toxin Mechanisms and Immunotoxins
- Corrosion Behavior and Inhibition
- Genomics and Chromatin Dynamics
- Ferrocene Chemistry and Applications
- Endoplasmic Reticulum Stress and Disease
Institute for Research in Biomedicine
2009-2023
Institut de Biologia Molecular de Barcelona
2003-2023
Consejo Superior de Investigaciones Científicas
2001-2023
Vitenparken
2012
Centre d’Investigació i Desenvolupament
2001-2003
University of Girona
1999-2001
Ellipticine is a natural plant product that has been found to be powerful anticancer drug. Although still unclear, its mechanism of action considered mainly based on DNA intercalation and/or the inhibition topoisomerase II. Many experimental data suggest an stacking interactions along major base-pair axis, but alternative binding modes have proposed, in particular for ellipticine derivatives. The 1.5 Å resolution structure complexed 6 bp oligonucleotide unveils mode and enables detailed...
Tubulin proteostasis is regulated by a group of molecular chaperones termed tubulin cofactors (TBC). Whereas heterodimer formation well-characterized biochemically, its dissociation pathway not clearly understood. We carried out biochemical assays to dissect the role human TBCE and TBCB in αβ-tubulin dissociation. used electron microscopy image processing determine three-dimensional structure TBCE, α-tubulin (αEB) complex, which formed via two chaperones. Docking atomic structures domains...
Variolin B is a rare marine alkaloid that showed promising anti-cancer activity soon after its isolation. It acts as cyclin-dependent kinase inhibitor, although the precise mechanism through which it exerts cytotoxic effects still unknown. The crystal structure of variolin bound to DNA forming pseudo-Holliday junction shows this compound can also contribute, intercalative binding, either formation or stabilization multi-stranded forms.
Abstract The PhoR-PhoB phosphorelay is a bacterial two-component system that activates the transcription of several genes involved in phosphate uptake and assimilation. response begins with autophosphorylation sensor kinase PhoR, which regulator PhoB. Upon binding to pho box DNA sequence, PhoB recruits RNA polymerase thereby specific genes. To unveil hitherto unknown molecular mechanisms along activation pathway, we report biochemical data characterizing promoters containing multiple boxes...
ToxR, a Vibrio cholerae transmembrane one-component signal transduction factor, lies within regulatory cascade that results in the expression of ToxT, toxin coregulated pilus, and cholera toxin. While ToxR has been extensively studied for its ability to activate or repress various genes V. , here we present crystal structures cytoplasmic domain bound DNA at toxT ompU promoters. The confirm some predicted interactions, yet reveal other unexpected promoter interactions with implications...
Adenosine deaminase acting on transfer RNA (ADAT) is an essential eukaryotic enzyme that catalyzes the deamination of adenosine to inosine at first position tRNA anticodons. Mammalian ADATs modify eight different tRNAs, having increased their substrate range from a bacterial ancestor likely deaminated exclusively tRNAArg Here we investigate recognition mechanisms and tRNAAla by human ADAT shed light process expansion took place during evolution enzyme. We show does not depend conserved...
Xylanases hydrolyze the beta-1,4-linked xylose backbone of xylans. They are increasing interest in paper and food industries for their pre-bleaching bio-pulping applications. Such demand new xylanases to cover a wider range cleavage specificity, activity stability. The catalytic domain xylanase Xys1 from Streptomyces halstedii JM8 was expressed, purified crystallized native data were collected 1.78 A resolution with an R(merge) 4.4%. crystals belong space group P2(1)2(1)2(1), unit-cell...
Mouse liver glutathione transferase P1-1 has three cysteine residues at positions 14, 47 and 169. We have constructed the single, double triple to alanine mutants define behaviour of all thiols. confirm that C47 is 'fast' thiol (pK 7.4), C169 as alkaline reactive residue with a pK(a) 8.6. Only small proportion C14 5,5'-dithiobis-(2-nitrobenoic acid) (DTNB) pH 9 in C47A/C169A mutant. The native enzyme C169A mutant exhibited Michaelis-Menten kinetics, but other sigmoidal kinetics varying...