A5060432507- Photosynthetic Processes and Mechanisms
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Cancer Genomics and Diagnostics
- Lung Cancer Treatments and Mutations
- Mitochondrial Function and Pathology
- Cancer Cells and Metastasis
- Microtubule and mitosis dynamics
- Connective tissue disorders research
- Colorectal Cancer Treatments and Studies
- Fungal and yeast genetics research
- Bone Tissue Engineering Materials
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- Gastric Cancer Management and Outcomes
- Cellular transport and secretion
- Cholinesterase and Neurodegenerative Diseases
- Microbial Metabolic Engineering and Bioproduction
- Polymer Surface Interaction Studies
- biodegradable polymer synthesis and properties
- Antioxidant Activity and Oxidative Stress
- Elasticity and Material Modeling
- Ion channel regulation and function
La Ligue Contre le Cancer
2019-2022
Université de Montpellier
2008-2022
Centre National de la Recherche Scientifique
1994-2022
Bruce W. Carter VA Medical Center
2022
Inserm
2020-2022
Institut de Génétique Moléculaire de Montpellier
2016-2021
Centro Oncológico de Galicia
2021
Hospital Universitario HM Sanchinarro
2015-2019
Centre National pour la Recherche Scientifique et Technique (CNRST)
2016
Universidad Nacional Autónoma de México
2016
<h3>Importance</h3> Although vitamin E and memantine have been shown to beneficial effects in moderately severe Alzheimer disease (AD), evidence is limited mild moderate AD. <h3>Objective</h3> To determine if (alpha tocopherol), memantine, or both slow progression of AD patients taking an acetylcholinesterase inhibitor. <h3>Design, Setting, Participants</h3> Double-blind, placebo-controlled, parallel-group, randomized clinical trial involving 613 with initiated August 2007 concluded...
Antigen Ki-67 is a nuclear protein expressed in proliferating mammalian cells. It widely used cancer histopathology but its functions remain unclear. Here, we show that controls heterochromatin organisation. Altering expression levels did not significantly affect cell proliferation vivo. mutant mice developed normally and cells lacking proliferated efficiently. Conversely, upregulation of differentiated tissues prevent cycle arrest. interactors included proteins involved nucleolar processes...
<h3>Importance</h3> Selecting effective antidepressants for the treatment of major depressive disorder (MDD) is an imprecise practice, with remission rates about 30% at initial treatment. <h3>Objective</h3> To determine whether pharmacogenomic testing affects antidepressant medication selection and such leads to better clinical outcomes. <h3>Design, Setting, Participants</h3> A pragmatic, randomized trial that compared guided by vs usual care. Participants included 676 clinicians 1944...
The spindle checkpoint prevents anaphase onset until all the chromosomes have successfully attached to microtubules. mechanisms by which unattached kinetochores trigger and transmit a primary signal are poorly understood, although it seems be dependent at least in part, on kinetochore localization of different components. By using protein immunodepletion mRNA translation Xenopus egg extracts, we studied hierarchic sequence interdependent network that governs recruitment pathway. Our results...
Abstract Background Colon cancer patients with the same stage show diverse clinical behavior dueto tumor heterogeneity. We aimed to discover distinct classes of tumorsbased on microarray expression patterns, analyze whether molecularclassification correlated histopathological stages or otherclinical parameters and study differences in survival. Methods Hierarchical clustering was performed for class discovery 88 colon tumors(stages I IV). Pathways analysis correlations between...
Adaptive therapy (AT) aims to control tumour burden by maintaining therapy-sensitive cells exploit their competition with resistant cells. This relies on the assumption that have impaired cellular fitness. Here, using a model of resistance pharmacological cyclin-dependent kinase inhibitor (CDKi), we show this is valid when between spatially structured. We generate CDKi-resistant cancer and find they reduced proliferative fitness stably rewired cell cycle pathways. Low-dose CDKi outperforms...
Significance Ki-67 is a nuclear protein present in all proliferating vertebrate cells and widely used as marker clinical cancer histopathology. However, its cellular functions have remained largely mysterious, whether it plays any roles was unknown. In this work, we show genetically that not required for cell proliferation tumors, but stages of carcinogenesis. The effects on transformation, tumor growth, metastasis, drug sensitivity correlate with genome-scale changes gene expression modify...
We have conducted a proteomic analysis of the 70 S ribosome from Chlamydomonas reinhardtii chloroplast. Twenty-seven orthologs Escherichia coli large subunit proteins were identified in 50 subunit, as well an ortholog spinach plastid-specific ribosomal protein-6. Several C. short extension or insertion sequences, but overall are very similar to those chloroplast and E. coli. Two 38 41 kDa, designated RAP38 RAP41, that not found either subunits. Phylogenetic RAP41 paralogs CSP41, RNA-binding...
Biomimetic hybrid materials based on a polymeric and an inorganic component such as calcium phosphate are potentially useful for bone repair. The current study reports new approach toward biomimetic using set of recombinamers (recombinant protein obtained from synthetic gene) crystallization additive phosphate. contain elements elastin, elastic structural protein, statherin, salivary protein. Via genetic engineering, the basic elastin sequence was modified with SN(A)15 domain whose...
// Rodrigo A. Toledo 1,4,* , Antonio Cubillo 1,2,* Estela Vega 1,2 Elena Garralda Rafael Alvarez Lisardo U. de la Varga Jesús R. Pascual Gema Sánchez 1,4 Francesca Sarno Susana H. Prieto 3 Sofía Perea Pedro P. Lopéz-Casas 4 Fernando López-Ríos and Manuel Hidalgo 4,5 1 Centro Integral Oncológico Clara Campal (CIOCC), Madrid, Spain 2 Universidad San Pablo CEU, Laboratorio Dianas Terapeúticas, Spanish National Cancer Research Centre (CNIO), 5 Beth Israel Deaconess Medical Center, Harvard...
The uncoupling protein (UCP) of brown adipose tissue is a regulated proton carrier which allows mitochondrial respiration from ATP synthesis and, therefore, dissipation metabolic energy as heat. In this article we demonstrate that, when UCP expressed in Saccharomyces cerevisiae, it retains all its functional properties: and chloride transport, high-affinity binding nucleotides regulation conductance by fatty acids. Site-directed mutagenesis demonstrates that sequential replacement serine...
Abstract Background Alzheimer's disease (AD) has been associated with both oxidative stress and excessive glutamate activity. A clinical trial was designed to compare the effectiveness of ( i ) alpha‐tocopherol, a vitamin E antioxidant; ii memantine (Namenda), an N‐methyl‐D‐aspartate antagonist; iii their combination; iv placebo in delaying progression AD. Methods The Veterans Affairs Cooperative Studies Program initiated multicenter, randomized, double‐blind, placebo‐controlled August 2007,...
To understand how chloroplast mRNAs are translated into functional proteins, a detailed understanding of all the components translation is needed. this end, we performed proteomic analysis plastid ribosomal proteins in small subunit ribosome from green alga Chlamydomonas reinhardtii. Twenty were identified, including orthologs Escherichia coli S1, S2, S3, S4, S5, S6, S7, S9, S10, S12, S13, S14, S15, S16, S17, S18, S19, S20, and S21 homolog spinach plastid-specific protein-3 (PSRP-3). In...
Abstract CDK8 and CDK19 form a conserved cyclin‐dependent kinase subfamily that interacts with the essential transcription complex, Mediator, also phosphorylates C‐terminal domain of RNA polymerase II. Cells lacking either or are viable have limited transcriptional alterations, but whether two kinases redundantly control cell proliferation differentiation is unknown. Here, we find in mice dispensable for regulation gene expression, normal intestinal homeostasis, efficient tumourigenesis,...
The growth of Saccharomyces cerevisiae cells under aerobic conditions, in the presence an energy-rich source, leads to production excess NAD(P)H. Since redox balance must be maintained, it has been postulated that NAD(P)H reoxidation is accelerated by activation energy-dissipating reactions, which would, turn, explain low efficiencies observed. It demonstrated already S. cultures these putative reactions are stimulated both oxygen and high cytosolic ATP levels. In this paper, we show induces...
We have recently reported that ATP induces an uncoupling pathway in Saccharomyces cerevisiae mitochondria [Prieto, Bouillaud, Ricquier and Rial (1992) Eur. J. Biochem. 208, 487-491]. The presence of this would explain the low efficiency oxidative phosphorylation S. cerevisiae, may represent one postulated energy-dissipating mechanisms present these yeasts. In paper we demonstrate exerts its action two steps: first, at ATP/Pi ratios, it increases respiratory-chain activity, probably by...
A number of cecropin‐A–melittin hybrid peptides have previously been shown to be potent antibacterial agents [Andreu, D., Ubach, J., Boman, A., Wahlin, B., Wade, Merrifield, R. B. & H. G. (1992) FEBS Lett. 296 , 190–1941. In the present report we analyze their action on biological systems using rat liver mitochondria as a test system. We demonstrate that longest peptide, cecropin‐A‐(1–8)‐melittin(l–18) permeabilizes mitochondrial inner membrane allowing movement both charged and...
Abstract When S. cerevisiae are grown with glucose, SDH2 mRNA encoding the iron protein of succinate dehydrogenase complex is unstable and present at low level. In yeast without stable its level rises. Addition glucose to a glucose‐limited culture causes fall rapidly half‐life ∼5–7 min. Previously 5′UTR was shown be necessary sufficient destabilize it in (Lombardo et al ., 1992 ). We now show that SDH1 SUC2 5′UTRs capable conferring glucose‐sensitive instability. also examine how changes...