A5071068240- Vitamin C and Antioxidants Research
- Cancer Cells and Metastasis
- Hereditary Neurological Disorders
- Vitamin K Research Studies
- Mitochondrial Function and Pathology
- Vector-borne infectious diseases
- Cellular Mechanics and Interactions
- Vitamin D Research Studies
- Genomics and Chromatin Dynamics
- DNA Repair Mechanisms
- Zebrafish Biomedical Research Applications
- Epigenetics and DNA Methylation
- Mosquito-borne diseases and control
- Nanoparticles: synthesis and applications
- RNA modifications and cancer
- Heat shock proteins research
- Endoplasmic Reticulum Stress and Disease
- Genetics and Neurodevelopmental Disorders
- RNA Research and Splicing
- Vibrio bacteria research studies
- Hippo pathway signaling and YAP/TAZ
- Neurogenesis and neuroplasticity mechanisms
- Nerve injury and regeneration
- Cancer therapeutics and mechanisms
- Pluripotent Stem Cells Research
Université de Montpellier
2019-2024
Centre National de la Recherche Scientifique
2019-2024
Inserm
2013-2024
Institut de Recherche en Cancérologie de Montpellier
2019-2022
Aix-Marseille Université
2011-2016
Nutrition, Obésité et Risque Thrombotique
2013-2014
Institut National de la Recherche Agronomique du Niger
2014
Château Gombert
2013
Significance Ki-67 is a nuclear protein present in all proliferating vertebrate cells and widely used as marker clinical cancer histopathology. However, its cellular functions have remained largely mysterious, whether it plays any roles was unknown. In this work, we show genetically that not required for cell proliferation tumors, but stages of carcinogenesis. The effects on transformation, tumor growth, metastasis, drug sensitivity correlate with genome-scale changes gene expression modify...
Tumor recurrence is often attributed to cancer stem cells (CSCs). We previously demonstrated that down-regulation of Pregnane X Receptor (PXR) decreases the chemoresistance CSCs and prevents colorectal recurrence. Currently, no PXR inhibitor usable in clinic. Here, we identify miR-148a as a targetable element upstream signaling CSCs, which when over-expressed expression impairs tumor relapse after chemotherapy mouse xenografts. then develop fluorescent reporter screen for activators...
Ascorbic acid (AA) has been considered, for a long time, only as the active principle of vitamin C, with antioxidant properties. We recently demonstrated that ascorbic is competitive inhibitor adenylate cyclases, acting global regulator cAMP pool. It was thus interesting to determine new targets AA, linked its potential signalling capacity, especially during cell differentiation. here AA an preadipocyte lines This inhibition dosage dependent. In addition, we describe impact on expression...
Intracellular bacterial pathogens divert multiple cellular pathways to establish their niche and persist inside host. Coxiella burnetii , the causative agent of Q fever, secretes effector proteins via its Type 4 secretion system generate a -containing vacuole (CCV). Manipulation lipid protein trafficking by these effectors is essential for replication virulence. Here, we have characterized composition CCVs found that Vice interacts with phosphoinositides membranes enriched in...
Colorectal cancer (CRC) ranks third among the most frequent malignancies and represents second common cause of cancer-related deaths worldwide. By interfering with DNA replication process cells, several chemotherapeutic molecules used in CRC therapy induce stress (RS). At cellular level, this is managed by ATR-CHK1 pathway, which activates checkpoint. In recent years, therapeutic value targeting pathway has been demonstrated. Moreover, MSI + (microsatellite instability) tumors frequently...
Scope Here we tested the hypothesis that ascorbic acid (AA) is a signaling molecule acting on stem cells via differentiation of mesoderm derivatives, including myocytes, osteocytes, and adipocytes. Material methods Investigations used murine embryonic cell line CGR8 able to differentiate into different types treated or not with acid. Differentiation was tracked mainly through cellular anatomy (including presence beating cardiomyocytes) expression specific markers. Conclusion The study...
// Michalis Fragkos 1 , 2 * Viviana Barra Tom Egger Benoit Bordignon Delphine Lemacon 3 Valeria Naim # and Arnaud Coquelle IRCM, Institut de Recherche en Cancérologie Montpellier, INSERM U1194, Université Régional du Cancer France Laboratory of Genetic Instability Oncogenesis, UMR 8200 CNRS, University Paris-Sud, Gustave Roussy, Villejuif, Present address: Department Biochemistry Molecular Biology, Doisy Research Center, St. Louis, MO, USA These authors contributed equally to this work Lead...
Mitochondria are essential organelles that play crucial roles in cellular energy metabolism, calcium signaling and apoptosis. Their importance tissue homeostasis stress responses, combined to their ability transition between various structural functional states, make them excellent for monitoring health. Quantitative assessment of mitochondrial morphology can therefore provide valuable insights into environmentally-induced cell damage. High-content screening (HCS) provides a powerful tool...
Abstract The X‐linked form of Charcot‐Marie‐Tooth disorder (CMTX) is the second most frequent type (15% CMT forms). It involves GJB1 gene coding for connexin 32, a protein involved in gap junction formation and function. There no curative treatment CMTX. We present data on transgenic lines that was accomplished by inserting human BAC carrying gene, which two different mutations 32 (Cx32) observed patients were introduced. Investigation these models implicated Cx32 control mitotic stability....
Mutation in the Gjb1 gene, coding for a connexin (Cx32), is associated with an inherited peripheral neuropathic disorder (X-linked Charcot-Marie-Tooth, CMTX). Our previous work reported that transgenic animals expressing human transgene present polyploidy and abnormal over-duplication of centrosome, suggesting role mitotic stability. In this article, we propose mechanisms by which mutations induce instability discuss its potential relation CMTX phenotype. We showed cells exhibit CamKII...
The zebrafish ( Danio rerio ) is a vertebrate species offering multitude of advantages for the study conserved biological systems in human and has considerably enriched our knowledge developmental biology physiology. Being equally important medical research, become critical tool fields diagnosis, gene discovery, disease modeling, pharmacology-based therapy. Studies on neuromuscular system allowed deciphering key molecular pathways this tissue, established it as model choice to numerous motor...
We previously described that fibroblasts from animal models of CMTX1 present genomic instability and poor connexon activity. In vivo, these transgenic mice motor deficits. This phenotype could be significantly reverted by treatment with (CamKII) inhibitors. The objective this study is to translate our findings patients. cultured skin biopsies patients analyzed cells for instabilty, activity, potential correction CamKII phenotypic analysis confirmed strong similarities between the GJB1 mouse...
Abstract Intracellular bacterial pathogens divert multiple cellular pathways to establish their niche and persist inside host. Coxiella burnetii , the causative agent of Q fever, secretes effector proteins via its Type 4 secretion system generate a -containing vacuole (CCV). Manipulation lipid protein trafficking by these effectors is essential for replication virulence. Here, we have characterized composition CCVs discovered that Vice interacts with phosphoinositides membranes enriched in...
ABSTRACT Recent studies have shown that the cell proliferation antigen Ki-67 is not required for proliferation. Here, we demonstrate enables implementation of transcriptional programmes conferring cellular plasticity, and each step tumour initiation, growth metastasis. knockout causes global transcriptome remodelling, which, in mammary carcinoma cells, inhibits epithelial-mesenchymal transition a polycomb-repressive complex 2-dependent manner. This results suppression stem characteristics...