A5086353622- Vector-borne infectious diseases
- Mosquito-borne diseases and control
- Toxoplasma gondii Research Studies
- Plant Genetic and Mutation Studies
- Viral Infections and Vectors
- Enzyme function and inhibition
- Genomics and Phylogenetic Studies
- Vector-Borne Animal Diseases
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Yersinia bacterium, plague, ectoparasites research
- Soybean genetics and cultivation
- Carbohydrate Chemistry and Synthesis
- Insect and Pesticide Research
- Glycosylation and Glycoproteins Research
- Plant Stress Responses and Tolerance
- Celiac Disease Research and Management
- Photosynthetic Processes and Mechanisms
- Plant responses to water stress
- Seed Germination and Physiology
- Bacterial Identification and Susceptibility Testing
- Toxin Mechanisms and Immunotoxins
- Advanced Proteomics Techniques and Applications
- Wheat and Barley Genetics and Pathology
- Plant tissue culture and regeneration
- Insect symbiosis and bacterial influences
Slovak Academy of Sciences
2007-2025
Public Health Authority of the Slovak Republic
2025
Institute of Virology of the Slovak Academy of Sciences
2014-2024
Biomedical Research Center of the Slovak Academy of Sciences
2016-2024
Czech Academy of Sciences
2014-2024
Czech Academy of Sciences, Institute of Microbiology
2015-2024
Institute of Crop Science
2010
National Agriculture and Food Research Organization
2010
Sorbonne Université
2010
Institute of Plant Genetics and Biotechnology of the Slovak Academy of Sciences
2009-2010
Flooding injury is a major problem in soybean cultivation. A proteomics approach was used to clarify the occurrence of changes protein expression level and phosphorylation soybeans under flooding stress. Two-day-old seedlings were flooded for 1 day, proteins extracted from root tips digested with trypsin, their levels states compared those untreated controls using mass spectrometry-based techniques. Phosphoproteins enriched phosphoprotein purification column prior digestion spectrometry. The...
Gel-based and gel-free proteomics techniques were used to investigate early responses flooding stress in the roots hypocotyls of soybean seedlings. Proteins from 2-day-old seedlings flooded for 12 h extracted analyzed. Two mass-spectroscopy-based analyses, two-dimensional fluorescence difference gel electrophoresis, nanoliquid chromatography identified 32 17 spots 81 proteins, respectively, as responsive stress. On basis number function proteins identified, glycolysis fermentation enzymes...
Coxiella burnetii is the causative agent of Q fever. The bacterium highly infectious and classified as a category B biological weapon. tools molecular biology are utmost importance in rapid unambiguous identification C. naturally occurring fever outbreaks, or cases deliberate release agent. In this work, development multiple locus variable number tandem repeats (VNTR) analysis (MLVA) for characterization described. Sixteen isolates five passage history/laboratory variants were characterized....
The explosion in one of the four reactors Chernobyl Nuclear Power Plant (CNPP, Chernobyl) caused worst nuclear environmental disaster ever seen. Currently, 23 years after accident, soil close vicinity CNPP is still significantly contaminated with long-living radioisotopes, such as (137)Cs. Despite this contamination, plants growing area were able to adapt radioactivity, and survive. aim study was investigate plant adaptation mechanisms toward permanently increased level radiation using a...
Natural foci of tick-borne spotted fever group (SFG) rickettsiae public health concern have been found in Slovakia, but the role rodents their circulation is unclear. Ticks (Ixodes ricinus, Ixodes trianguliceps, Dermacentor marginatus, reticulatus, Haemaphysalis concinna and inermis) tissues (Apodemus flavicollis, Apodemus sylvaticus, Myodes glareolus, Microtus arvalis, subterraneus Micromys minutus) were examined for presence SFG Coxiella burnetii by molecular methods. Suburban, natural...
The amount of clinically relevant, allergy-related proteins in wheat grain is still largely unknown. application proteomics may create a platform not only for identification and characterization, but also quantitation these proteins. aim this study was to evaluate the data-independent quantitative mass spectrometry (MSE) approach combination with 76 allergenic sequences downloaded from AllergenOnline database (www.allergenonline.org) as starting point. Alcohol soluble extracts gliadin...
Dual-specificity mitogen-activated protein kinases (MAPKKs) are the immediate upstream activators of MAPKs. They simultaneously phosphorylate TXY motif within activation loop MAPKs, allowing them to interact with and regulate multiple substrates. Often, MAPKs triggers their nuclear translocation. However, spatiotemporal dynamics physiological consequences particularly in plants, still poorly understood. Here, we studied localization Medicago sativa stress-induced MAPKK (SIMKK)–SIMK module...
The accident at the Chernobyl Nuclear Power Plant (CNPP) on April 26, 1986 is most serious nuclear disaster in human history. Surprisingly, while area proximal to CNPP remains substantially contaminated with long-lived radioisotopes including 90Sr and 137Cs, local ecosystem has been able adapt. To evaluate plant adaptation, seeds of a flax (Linum usitatissimum) variety Kyivskyi were sown radio-contaminated control fields region. A total protein fraction was isolated from mature seeds,...
Plants grow and reproduce in the radioactive Chernobyl area, however there has been no comprehensive characterization of these activities. Herein we report that life this environment led to alteration developing soybean seed proteome a specific way resulted production fertile seeds with low levels oil β-conglycinin storage proteins. Soybean were harvested at four, five, six weeks after flowering, maturity from plants grown either non-radioactive or plots area. The abundance 211 proteins was...
Haemosporidians are a group of vector-borne parasites belonging to the order Haemosporida. They infect avian hosts and require blood-sucking insects (Diptera) for their transmission. The occurrence diversity haemosporidian shaped primarily by specificity parasite susceptibility host/vector. In this study, presence distribution haemosporidians in blood samples from birds urbanized natural habitats were estimated using microscopic molecular approaches. Birds infected with four different...
Rapid and reliable detection, identification, typing of bacterial species are necessary in response to natural or terrorist-caused outbreaks infectious diseases play crucial roles diagnosis efficient treatment. We report here two proteomic approaches with a high potential the detection identification Coxiella burnetii, causative agent Q fever. The first them starts acetonitrile (ACN) trichloroacetic acid extractions inactivated C. burnetii cells followed by extracted molecules ions derived...
The Gram-negative pathogen Coxiella burnetii is an intracellular bacterium that replicates within the phagolysosomal vacuoles of eukaryotic cells. This can infect a wide range hosts, and causative agent Q fever in humans. Surface-exposed cell envelope associated proteins are thought to be important for both pathogenesis protective immunity. Herein, we propose complementary strategy consisting (i) silico prediction (ii) inventory proteomic composition using three enrichment approaches coupled...