A5041772839- Seed Germination and Physiology
- Plant Molecular Biology Research
- Soybean genetics and cultivation
- Plant tissue culture and regeneration
- Plant Genetic and Mutation Studies
- Plant Stress Responses and Tolerance
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Plant Parasitism and Resistance
- Allelopathy and phytotoxic interactions
- Plant Gene Expression Analysis
- Plant responses to water stress
- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Growth and nutrition in plants
- Plant Disease Resistance and Genetics
- Magnetic and Electromagnetic Effects
- RNA and protein synthesis mechanisms
- Genetic Mapping and Diversity in Plants and Animals
- Phytase and its Applications
- Horticultural and Viticultural Research
- Plant Micronutrient Interactions and Effects
- Plant Water Relations and Carbon Dynamics
- Turfgrass Adaptation and Management
Wageningen University & Research
2015-2025
Utrecht University
2008-2017
Graduate School Experimental Plant Sciences
2000-2016
University of Leeds
2012
The University of Melbourne
2012
Centre for BioSystems Genomics
2012
Laboratory of Molecular Genetics
2006
Research International (United States)
2002
Abstract Sugar-induced anthocyanin accumulation has been observed in many plant species. We that sucrose (Suc) is the most effective inducer of biosynthesis Arabidopsis (Arabidopsis thaliana) seedlings. Other sugars and osmotic controls are either less or ineffective. Analysis Suc-induced 43 accessions shows considerable natural variation exists for this trait. The Cape Verde Islands (Cvi) accession essentially does not respond to Suc, whereas Landsberg erecta an intermediate responder....
Genetic variation for seed dormancy in nature is a typical quantitative trait controlled by multiple loci on which environmental factors have strong effect. Finding the genes underlying major scientific challenge, also has relevance agriculture and ecology. In this study we describe identification of DELAY OF GERMINATION 1 (DOG1) gene previously identified as locus involved control dormancy. This was isolated combination positional cloning mutant analysis absolutely required induction DOG1...
Seed dormancy allows seeds to overcome periods that are unfavourable for seedling established and is therefore important plant ecology agriculture. Several processes known be involved in the induction of switch from dormant germinating state. The role hormones, different tissues genes involved, including newly identified germination described this chapter, as well use transcriptome, proteome metabolome analyses study these mechanistically not understood processes.
Quantifying gene expression levels is an important research tool to understand biological systems. Reverse transcription-quantitative real-time PCR (RT-qPCR) the preferred method for targeted measurements because of its sensitivity and reproducibility. However, normalization, necessary correct sample input reverse transcriptase efficiency, a crucial step obtain reliable RT-qPCR results. Stably expressed genes (i.e. whose not affected by treatment or developmental stage under study) are...
Seeds are unique time capsules that can switch between 2 complex and highly interlinked stages: seed dormancy germination. Dormancy contributes to the survival of plants because it allows delay germination optimal conditions. The occurs in response developmental environmental cues. In this review we provide a comprehensive overview studies have helped unravel molecular mechanisms underlying over last decades. Genetic physiological provided strong foundation for field research revealed...
Abstract Arabidopsis accessions differ largely in their seed dormancy behavior. To understand the genetic basis of this intraspecific variation we analyzed two accessions: laboratory strain Landsberg erecta (Ler) with low and strong-dormancy accession Cape Verde Islands (Cvi). We used a quantitative trait loci (QTL) mapping approach to identify affecting after-ripening requirement measured as number days dry storage required reach 50% germination. Thus, seven QTL were identified named delay...
In Arabidopsis recombinant inbred line (RIL) populations are widely used for quantitative trait locus (QTL) analyses. However, mapping analyses with this type of population can be limited because the masking effects major QTL and epistatic interactions multiple QTL. An alternative immortal experimental commonly in plant species sets introgression lines. Here we introduce development a genomewide coverage near-isogenic (NIL) thaliana, by introgressing genomic regions from Cape Verde Islands...
Abstract Seed oligosaccharides (OSs) and especially raffinose series OSs (RSOs) are hypothesized to play an important role in the acquisition of desiccation tolerance consequently seed storability. In present work we analyzed seed-soluble OS (sucrose, raffinose, stachyose) content several Arabidopsis accessions thus identified genotype Cape Verde Islands having a very low RSO content. By performing quantitative trait loci (QTL) mapping recombinant inbred line population, found one major QTL...
Timing of germination is presumably under strong natural selection as it determines the environmental conditions in which a plant germinates and initiates its postembryonic life cycle. To investigate how seed dormancy controlled, quantitative trait loci (QTL) analyses has been performed six Arabidopsis thaliana recombinant inbred line populations by analyzing them simultaneously using mixed model QTL approach. The were derived from crosses between reference accession Landsberg erecta (L er )...
Abstract Seed germination is a critical stage in the plant life cycle and first step toward successful establishment. Therefore, understanding of important ecological agronomical relevance. Previous research revealed that different seed compartments (testa, endosperm, embryo) control germination, but little known about underlying spatial temporal transcriptome changes lead to germination. We analyzed genome-wide expression germinating Arabidopsis (Arabidopsis thaliana) seeds with both detail...
The possible role of the sucrose-splitting enzymes sucrose synthase and invertase in elongating roots hypocotyls Arabidopsis was tested by using a combination histochemical methods quantitative trait locus (QTL) analysis. Lengths correlated better with activities than activities. highest correlations were observed zones roots. genetic basis these studied QTL Several loci, affecting activity, colocated loci that had an effect on root or hypocotyl length. Further fine mapping major for length,...
Seed performance after dispersal is highly dependent on parental environmental cues, especially during seed formation and maturation. Here we examine which factors are the most dominant in this respect whether their effects genotypes under investigation. We studied influence of light intensity, photoperiod, temperature, nitrate, phosphate development five plant attributes thirteen attributes, using 12 Arabidopsis that have been reported to be affected traits. As expected, various...
Summary The seed expressed gene DELAY OF GERMINATION ( DOG ) 1 is absolutely required for the induction of dormancy. Next to a non‐dormant phenotype, dog1‐1 mutant also characterized by reduced longevity suggesting that may affect additional processes as well. This aspect however, has been hardly studied and poorly understood. To uncover roles in seeds we performed detailed analysis dog1 using both transcriptomics metabolomics investigate molecular consequences dysfunctional gene. Further,...
Proteomics approaches have been a useful tool for determining the biological roles and functions of individual proteins identifying molecular mechanisms that govern seed germination, vigour viability in response to ageing. In this work dry proteome four Arabidopsis thaliana genotypes, carry introgression fragments at position longevity quantitative trait loci as result display different levels longevity, was investigated. Seeds two physiological states, after-ripened seeds had full...
Abstract Dormancy is a state of metabolic arrest that facilitates the survival organisms during environmental conditions incompatible with their regular course life. Many have deep dormant stages to promote an extended life span (increased longevity). In contrast, plants seed dormancy and longevity described as two traits. Seed defined temporary failure viable germinate in favor germination, whereas viability after dry storage (storability). plants, association has not been studied detail....
The temporal control or timing of the life cycle annual plants is presumed to provide adaptive strategies escape harsh environments for survival and reproduction. This mainly determined by germination, which controlled level seed dormancy, flowering initiation. However, environmental factors driving evolution plant cycles remain largely unknown. To address this question we have analysed nine quantitative history traits, in a native regional collection 300 wild accessions Arabidopsis...
Summary This work investigates the extent of translational regulation during seed germination. The polysome occupancy each gene is determined by genome‐wide profiling total mRNA and polysome‐associated . reveals extensive Arabidopsis thaliana thousands individual s changes to a large germination process. Intriguingly, these are restricted two temporal phases (shifts) germination, hydration Sequence features, such as upstream open reading frame number, transcript length, stability, secondary...
Abstract Seed dormancy determines germination timing and contributes to crop production the adaptation of natural populations their environment. Our knowledge about its regulation is limited. In a mutagenesis screen highly dormant Arabidopsis thaliana line, reduced dormancy5 (rdo5) mutant was isolated based on strongly seed dormancy. Cloning RDO5 showed that it encodes PP2C phosphatase. Several phosphatases belonging clade A are involved in abscisic acid signaling control However, does not...