Summary The seed expressed gene DELAY OF GERMINATION ( DOG ) 1 is absolutely required for the induction of dormancy. Next to a non‐dormant phenotype, dog1‐1 mutant also characterized by reduced longevity suggesting that may affect additional processes as well. This aspect however, has been hardly studied and poorly understood. To uncover roles in seeds we performed detailed analysis dog1 using both transcriptomics metabolomics investigate molecular consequences dysfunctional gene. Further,...

During seed germination, the transition from a quiescent metabolic state in dry mature to proliferative vigorous seedling is crucial for plant propagation as well optimizing crop yield. This work provides detailed description of dynamics protein synthesis during time course demonstrating that mRNA translation both sequential and selective this process. The complete inhibition germination process presence inhibitor cycloheximide established critical Arabidopsis germination. However, turnover...

Proteomics approaches have been a useful tool for determining the biological roles and functions of individual proteins identifying molecular mechanisms that govern seed germination, vigour viability in response to ageing. In this work dry proteome four Arabidopsis thaliana genotypes, carry introgression fragments at position longevity quantitative trait loci as result display different levels longevity, was investigated. Seeds two physiological states, after-ripened seeds had full...

Autophagy is essential for nutrient recycling and plays a fundamental role in seed production grain filling plants. participates nitrogen remobilization at the whole-plant level, seeds of autophagy mutants present abnormal C N contents relative to wild-type (WT) It well known that (ATG) genes are induced leaves during senescence; however, expression such has not yet been reported. In this study we show most ATG maturation Arabidopsis siliques. Promoter-ATG8f::UIDA promoter-ATG8f::GFP fusions...

Abscisic acid (ABA) is an important hormone for seed development and germination whose physiological action modulated by its endogenous levels. Cleavage of carotenoid precursors 9-cis epoxycarotenoid dioxygenase (NCED) inactivation ABA 8′-hydroxylase (CYP707A) are key regulatory metabolic steps. In Arabidopsis (Arabidopsis thaliana), both enzymes encoded multigene families, having distinctive expression patterns. To evaluate the genome-wide impact deficiency in developing seeds at maturation...

Autophagy is essential for protein degradation, nutrient recycling, and nitrogen remobilization. induced during leaf ageing in response to starvation, known play a fundamental role recycling remobilization seed filling. Accordingly, leaves of Arabidopsis autophagy mutants (atg) have been shown over-accumulate proteins peptides, possibly because reduced degradation capacity. Surprisingly, atg also displayed higher protease activities. The work reported here aimed at identifying the nature...

Summary Autophagy is a universal mechanism in eukaryotic cells that facilitates the degradation of unwanted cell constituents and essential for homeostasis nutrient recycling. The salicylic acid‐independent effects autophagy defects on leaf metabolism were determined through large‐scale proteomic lipidomic analyses atg5 atg5/sid2 mutants under different nitrogen sulfur growth conditions. Results revealed irrespective conditions, plants carrying mutation presented all characteristics...

Cell wall remodeling is an essential mechanism for the regulation of plant growth and architecture, xyloglucans (XyGs), major hemicellulose, are often considered as spacers cellulose microfibrils during growth. In seed, activity cell enzymes plays a critical role in germination by enabling embryo expansion leading to radicle protrusion, well endosperm weakening prior its rupture. A screen Arabidopsis (Arabidopsis thaliana) mutants affected hormonal control identified mutant, xyl1, able...

Although rice is a key crop species, few studies have addressed both seed physiological and nutritional quality, especially at the tissue level. In this study, an exhaustive "multi-omics" dataset on mature was obtained by combining transcriptomics, label-free shotgun proteomics metabolomics from embryo endosperm, independently. These high-throughput analyses provide new insight tissue-specificity related to quality. Foremost, we pinpointed that extensive post-transcriptional regulations...

Seed germination and subsequent seedling growth affect the final yield quality of crop. is defined as a series processes that begins with water uptake by quiescent dry seed ends elongation embryonic axis. Rice an important cereal crop species, during germination, two tissues function in different manner; embryo grows into next generation endosperm responsible for nutritional supply. Toward understanding integrated roles each tissue at transcriptional, translational, metabolic production...

Chimeric Antigen Receptor T (CAR-T) cells offer a promising strategy for cancer treatment. These CAR-T are either autologous or allogeneic that genetically modified to express chimeric antigen receptor targeting specific tumor antigen. Ongoing research aims optimize the cell efficacy, including strategies modulate their metabolism. One such approach involves inducing transgene expression by activating GCN2 kinase signaling pathway through dietary deprivation of an essential amino acid. In...

Obesity poses significant health and socioeconomic challenges, necessitating early detection of predisposition for effective personalized prevention. To identify candidate predictive markers, our study used two mouse models: one exhibiting interindividual variability in obesity another inducing metabolic phenotypes through maternal nutritional stresses. In both cases, was assessed by challenging mice with a high-fat diet. Using multivariate analyses transcriptomic data from white adipose...

Despite having very similar initial pools of stored mRNAs and proteins in the dry state, mature Arabidopsis seeds can either proceed toward radicle protrusion or stay a dormant state upon imbibition. Dormancy breaking, prerequisite to germination completion, be induced by different treatments though underlying mechanisms remain elusive. Thus, we investigated consequence such on seed proteome. Two unrelated dormancy-releasing were applied seeds, namely, cold stratification exogenous nitrates,...

On imbibition, Arabidopsis (

The retromer is a multiprotein complex conserved from yeast to humans, which involved in intracellular protein trafficking and recycling. Selection of cargo proteins transported by the depends on core subunit composed three vacuolar sorting (VPS) proteins, namely VPS26, VPS29, VPS35. To gain better knowledge importance plant sorting, we carried out comparative proteomic metabolomic analysis Arabidopsis thaliana seeds wild-type null-retromer mutant vps29. Here, report that displays major...

Most vacuolar proteins are synthesized on rough endoplasmic reticulum as proprotein precursors and then transported to the vacuoles, where they converted into their respective mature forms by processing enzymes (VPEs). In case of seed storage proteins, this process is major importance, it conditions establishment vigorous seedlings. Toward goal identifying proteome signatures that could be associated with origin early diversification angiosperms, we previously characterized 11S-legumin-type...

The Unfolded Protein Response (UPR) is a retrograde, ER-to-nucleus, signalling pathway which conserved across kingdoms. In plants, it contributes to development, reproduction, immunity and tolerance abiotic stress. This RNA sequencing (RNA-seq) dataset was produced from 14-day-old Arabidopsis thaliana seedlings challenged by tunicamycin (Tm), an antibiotic inhibiting Asn-linked glycosylation in the endoplasmic reticulum (ER), causing ER stress eventually activating UPR. Wild-type (WT) double...

Cancer cachexia is an involuntary loss of body weight, mostly skeletal muscle. Previous research favors the existence a microbiota-muscle crosstalk, so aim study was to evaluate impact microbiota alterations induced by antibiotics on muscle proteins expression. Skeletal proteome changes were investigated in control (CT) or C26 cachectic mice (C26) with without antibiotic treatment (CT-ATB C26-ATB,

Introduction Ductal carcinoma in situ (DCIS), characterized by a proliferation of neoplastic cells confined within the mammary ducts, is distinctly isolated from surrounding stroma an almost uninterrupted layer myoepithelial (MECs) and basement membrane. Heightened interactions adipose microenvironment, particularly obese patients, may play key role transition DCIS to invasive ductal (IDC), which attracting growing interest scientific research. Adipose tissue undergoes metabolic changes...

Desiccation tolerance allows plant seeds to remain viable in a dry state for years and even centuries. To reveal potential evolutionary processes of this trait, we have conducted shotgun proteomic analysis isolated embryo endosperm from mature Amborella trichopoda, an understory shrub endemic New Caledonia that is considered be the basal extant angiosperm. The present led characterization 415 69 proteins tissues, respectively. role these discussed terms protein evolution physiological...

In barley, incubation of primary dormant (D1) grains on water under conditions that do not allow germination, i.e. 30°C in air and 15°C or 5% O2, induces a secondary dormancy (D2) expressed as loss the ability to germinate at air. The aim this study was compare proteome barley embryos isolated from D1 D2 ones after induction hypoxia 30°C. Total soluble proteins were analyzed by 2DE gel-based proteomics, allowing selection 130 differentially accumulated (DAPs) among 1,575 detected spots....