A5002417724- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Musicology and Musical Analysis
- Cell death mechanisms and regulation
- Plant-Microbe Interactions and Immunity
- CRISPR and Genetic Engineering
- Plant Reproductive Biology
- Transgenic Plants and Applications
- Plant Genetic and Mutation Studies
- Autophagy in Disease and Therapy
- Plant Stress Responses and Tolerance
- Plant responses to elevated CO2
- Endoplasmic Reticulum Stress and Disease
- Phytase and its Applications
- Insect Resistance and Genetics
- Plant Gene Expression Analysis
- Plant and animal studies
- Insect-Plant Interactions and Control
- Light effects on plants
- Chromosomal and Genetic Variations
- Biochemical and Structural Characterization
- Plant responses to water stress
- Plant nutrient uptake and metabolism
- Arts, Culture, and Music Studies
University of Manchester
2011-2024
Université de Perpignan
1993-2004
Centre National de la Recherche Scientifique
1996-2003
Laboratoire Génome et Développement des Plantes
2000-2001
Rothamsted Research
1988-1992
College of West Anglia
1988
Programmed cell death (PCD) is a genetically controlled that regulated during development and activated in response to environmental stresses or pathogen infection. The degree of conservation PCD across kingdoms phylum not yet clear; however, whereas caspases are proteases act as key components animal apoptosis, plants have no orthologous caspase sequences their genomes. discovery plant fungi metacaspases most closely related led the hypothesis functional homologues these organisms....
Plants, animals, and several branches of unicellular eukaryotes use programmed cell death (PCD) for defense or developmental mechanisms. This argues a common ancestral apoptotic system in eukaryotes. However, at the molecular level, very few regulatory proteins protein domains have been identified as conserved across all eukaryotic PCD forms. A important goal is to determine which components may be used execution plants, during evolution, are plant-specific. Using Arabidopsis thaliana, we...
Abstract Programmed cell death (PCD) is used by plants for development and survival to biotic abiotic stresses. The role of caspases in PCD well established animal cells. Over the past 15 years, importance caspase-3-like enzymatic activity plant completion has been widely documented despite absence caspase orthologues. In particular, caspase-3 inhibitors blocked nearly all tested. Here, we affinity-purified a using biotin-labelled inhibitor identified Arabidopsis thaliana cathepsin B3...
With a view to studying programmed cell death in plants at the molecular level, we report here for first time that apoptotic‐like changes are induced by UV radiation plant nuclei. In Arabidopsis thaliana seedlings UV‐C dose of 10–50 kJ/m 2 induces an oligonucleosomal DNA fragmentation which is reminiscent apoptotic ladder described animal cells. This was also detected situ protoplast nuclei as soon h after treatment. Moreover, nuclear morphology characteristic We propose induction...
Autophagy is essential for protein degradation, nutrient recycling, and nitrogen remobilization. induced during leaf ageing in response to starvation, known play a fundamental role recycling remobilization seed filling. Accordingly, leaves of Arabidopsis autophagy mutants (atg) have been shown over-accumulate proteins peptides, possibly because reduced degradation capacity. Surprisingly, atg also displayed higher protease activities. The work reported here aimed at identifying the nature...
Abstract The Plastid Terminal Oxidase (PTOX) is a chloroplast localized plastoquinone oxygen oxidoreductase suggested to have the potential act as photoprotective safety valve for photosynthesis. However, PTOX overexpression in plants has been unsuccessful at inducing photoprotection, and factors that control its activity remain elusive. Here, we show significant induced response high light model species Eutrema salsugineum Arabidopsis thaliana . This activation correlates with structural...
Summary Programmed cell death (PCD) is fundamentally important for plant development, abiotic stress responses and immunity, but our understanding of its regulation remains fragmented. Building a stronger research community required to accelerate progress in this area through knowledge exchange constructive debate. In Viewpoint, we aim initiate collective effort integrate data across diverse set experimental models facilitate characterisation the fundamental mechanisms underlying PCD...
Programmed cell death or apoptosis is a process in which unwanted cells are eliminated during growth and development. In mammals, several genes have been identified whose products necessary to prevent entry into the apoptotic process. We isolated clone from an Arabidopsis thaliana cDNA library predicted translation product shows highly significant similarity mammalian defender against 1 (DAD1) protein. Transformation of mutant hamster tsBN7 cells, undergo at restrictive temperature,...
Community genetics examines how genotypic variation within a species influences the associated ecological community. The inclusion of additional environmental and factors is natural extension current community framework. However, extent to which presence genetic in interspecific interactions (i.e., genotype x environment [G G E] interactions) has been largely ignored. We used approach study interaction barley aphids absence rhizosphere bacteria. designed matrix aphid combinations found...
Journal Article Transformation of Sugarbeet (Beta vulgaris) by Agrobacterium tumefaciens K. LINDSEY, LINDSEY 3 1Leicester Biocentre, University LeicesterLeicester LE1 7RH, UK To whom correspondence should be addressed. Search for other works this author on: Oxford Academic PubMed Google Scholar P. GALLOIS 2AFRC IACR, Department Biochemistry, Rothamsted Experimental StationHarpenden, Herts., AL5 2JQ, 4 Present address: Laboratoire de Physiologie Vegetale, Universite Perpignan, France. Author...
Programmed cell death (PCD) induced by endoplasmic reticulum (ER) stress is implicated in various plant physiological processes, yet its mechanism still elusive. An activation of caspase-3-like enzymatic activity was clearly demonstrated but the role two known proteases with activity, cathepsin B and proteasome subunit PBA1, remains to be established. Both genetic downregulation chemical inhibition were used investigate function PBA1 ER-stress-induced PCD (ERSID). Transcript level labelling...
RNA is subject to many modifications, from small chemical changes such as methylation through conjugation of biomolecules glycans. As well these endogenously written also exposed damage induced by its environment. Certain clinical compounds are known drive covalent modifications with a growing appreciation for how affect function. To understand the regulation we need reliable, sensitive and rapid methodology their quantification. Thus, developed AquIRE applied it analysis drug-induced...
AbstractD.C. Sigee, A. Selwyn, P. Gallois and A.P. Dean. 2007. Patterns of cell death in freshwater colonial cyanobacteria during the late summer bloom. Phycologia 46: 284–292. DOI: 10.2216/06-69.1The occurrence senescence (Evans blue staining) programmed (Hoechst staining/TUNEL reaction) was studied Anabaena flos-aquae Microcystis bloom a eutrophic lake. Algae were analysed over seven-day period (three sampling days) within mixed phytoplankton samples obtained from upper epilimnion...