A5040102902- Photosynthetic Processes and Mechanisms
- Photoreceptor and optogenetics research
- Plant Stress Responses and Tolerance
- Algal biology and biofuel production
- Light effects on plants
- Mitochondrial Function and Pathology
- Micro and Nano Robotics
- Cell death mechanisms and regulation
- Plant-Microbe Interactions and Immunity
- ATP Synthase and ATPases Research
- GABA and Rice Research
- Plant Molecular Biology Research
- Redox biology and oxidative stress
- Marine Invertebrate Physiology and Ecology
- CAR-T cell therapy research
- Plant responses to elevated CO2
- Plant Physiology and Cultivation Studies
- Retinal Development and Disorders
- Plant tissue culture and regeneration
- Virus-based gene therapy research
- Plant responses to water stress
- Plant nutrient uptake and metabolism
- Neurobiology and Insect Physiology Research
- interferon and immune responses
- Autophagy in Disease and Therapy
Sorbonne Université
2012-2024
Centre National de la Recherche Scientifique
2013-2024
Institut de Biologie Paris-Seine
2021-2024
Institut de Biologie Physico-Chimique
2013-2023
Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes
2013-2023
Laboratoire de Biologie Computationnelle et Quantitative
2016-2023
Institut des Sciences des Plantes de Paris Saclay
2013-2016
Université Claude Bernard Lyon 1
2016
UPMC Health System
2014
École Polytechnique Fédérale de Lausanne
2003-2009
Abstract The conditional fluorescent (flu) mutant of Arabidopsis accumulates the photosensitizer protochlorophyllide in dark. After a dark-to-light shift, generation singlet oxygen, nonradical reactive oxygen species, starts within first minute illumination and was shown to be confined plastids. Immediately after plants stopped growing developed necrotic lesions. These early stress responses flu do not seem result merely from physicochemical damage. Peroxidation chloroplast membrane lipids...
Microalgae are regarded as promising organisms to develop innovative concepts based on their photosynthetic capacity that offers more sustainable production than heterotrophic hosts. However, realize potential green cell factories, a major challenge is make microalgae easier engineer. A approach for rapid and predictable genetic manipulation use standardized synthetic biology tools workflows. To this end we have developed Modular Cloning toolkit the microalga Chlamydomonas reinhardtii. It...
Plants, animals, and several branches of unicellular eukaryotes use programmed cell death (PCD) for defense or developmental mechanisms. This argues a common ancestral apoptotic system in eukaryotes. However, at the molecular level, very few regulatory proteins protein domains have been identified as conserved across all eukaryotic PCD forms. A important goal is to determine which components may be used execution plants, during evolution, are plant-specific. Using Arabidopsis thaliana, we...
Summary Upon a dark/light shift the conditional flu mutant of Arabidopsis starts to generate singlet oxygen ( 1 O 2 ), non‐radical reactive species that is restricted plastid compartment. Immediately after shift, plants stop growing and develop necrotic lesions. We have established protoplast system, which allows detection characterization death response in induced by release . Vitamin B6 quenches fungi was able protect protoplasts from cell death. Blocking ethylene production sufficient...
Mitochondrial NADH-ubiquinone oxidoreductase (complex I) is the largest enzyme of oxidative phosphorylation system, with subunits located at matrix and membrane domains. In plants, holocomplex I composed more than 40 subunits, 9 which are encoded by mitochondrial genome (NAD subunits). Nicotiana sylvestris, a minor 800-kDa subcomplex containing both domains displaying NADH dehydrogenase activity detectable. The NMS1 mutant lacking arm NAD4 subunit CMSII peripheral NAD7 devoid holoenzyme....
Abstract Programmed cell death (PCD) is used by plants for development and survival to biotic abiotic stresses. The role of caspases in PCD well established animal cells. Over the past 15 years, importance caspase-3-like enzymatic activity plant completion has been widely documented despite absence caspase orthologues. In particular, caspase-3 inhibitors blocked nearly all tested. Here, we affinity-purified a using biotin-labelled inhibitor identified Arabidopsis thaliana cathepsin B3...
With a view to studying programmed cell death in plants at the molecular level, we report here for first time that apoptotic‐like changes are induced by UV radiation plant nuclei. In Arabidopsis thaliana seedlings UV‐C dose of 10–50 kJ/m 2 induces an oligonucleosomal DNA fragmentation which is reminiscent apoptotic ladder described animal cells. This was also detected situ protoplast nuclei as soon h after treatment. Moreover, nuclear morphology characteristic We propose induction...
Programmed cell death (PCD) plays an important role during the life cycle of higher organisms. Although several regulatory mechanisms governing PCD are thought to be conserved in animals and plants, light-dependent represents a form that is unique plants. The light requirement has often been associated with production reactive oxygen species photosynthesis. In support this hypothesis, hydrogen peroxide superoxide have shown involved triggering response. present work, we used conditional flu...
Cardiolipin (CL) is the signature phospholipid of mitochondrial inner membrane. In animals and yeast (Saccharomyces cerevisiae), CL depletion affects stability respiratory supercomplexes thus crucial to energy metabolism obligate aerobes. eukaryotes, last step synthesis catalyzed by CARDIOLIPIN SYNTHASE (CLS), encoded a single-copy gene. Here, we characterize cls mutant in Arabidopsis thaliana, which devoid CL. contrast cls, where development little affected, seedlings are slow developing...
Upon a dark/light shift the conditional flu mutant of Arabidopsis starts to generate singlet oxygen (1O2) that is restricted plastid compartment. Distinct sets genes are activated different from those induced by hydrogen peroxide/superoxide. One rapidly upregulated EDS1 (enhanced disease susceptibility). The protein has been shown be required for resistance biotrophic pathogens and accumulation salicylic acid (SA) enhances defenses plant inducing synthesis pathogen-related (PR) proteins....
Sugars modulate many vital metabolic and developmental processes in plants, from seed germination to flowering, senescence protection against diverse abiotic biotic stresses. However, the exact mechanisms involved morphogenesis, signalling stress tolerance remain largely unknown. Here we report characterization of a novel Arabidopsis thaliana mutant, sweetie, with drastically altered strongly modified carbohydrate metabolism leading elevated levels trehalose, trehalose-6-phosphate starch. We...
The flu mutant of Arabidopsis thaliana overaccumulates in the dark immediate precursor chlorophyllide, protochlorophyllide (Pchlide), a potent photosensitizer, that upon illumination generates singlet oxygen (1O2). Once 1O2 has been released plastids mutant, mature plants stop growing, while seedlings die. Several suppressor mutations, dubbed oxygen-linked death activator (soldat), were identified specifically abrogate 1O2-mediated stress responses young without grossly affecting plants. One...
Multicellular organisms implement a set of reactions involving signaling and cooperation between different types cells. Unicellular organisms, on the other hand, activate defense systems that involve collective behaviors individual organisms. In unicellular model alga Chlamydomonas (Chlamydomonas reinhardtii), existence function mechanisms in response to stress remain mostly at level formation small structures called palmelloids. Here, we report characterization mechanism abiotic can trigger...
Abstract Among the crucial processes that preside over destiny of cells from any type organism are those involving their self-destruction. This process is well characterized and conceptually logical to understand in multicellular organisms; however, levels knowledge comprehension its existence still quite enigmatic unicellular organisms. We use Chlamydomonas (Chlamydomonas reinhardtii) lay foundation for understanding mechanisms programmed cell death (PCD) a photosynthetic organism. In this...
Cryptochromes are conserved flavoprotein receptors found throughout the biological kingdom with diversified roles in plant development and entrainment of circadian clock animals. Light perception is proposed to occur through flavin radical formation that correlates activity vivo both plants Drosophila. By contrast, mammalian (Type II) cryptochromes regulate independently light, raising fundamental question whether have evolved entirely distinct signaling mechanisms. Here we show by...
Chlamydomonas reinhardtii is a model unicellular organism for basic or biotechnological research, such as the production of high-value molecules biofuels thanks to its photosynthetic ability. To enable rapid construction and optimization multiple designs strains, our team collaborators have developed versatile Modular Cloning toolkit comprising 119 biobricks. Having ability use wide range selectable markers an important benefit forward reverse genetics in Chlamydomonas. We report here...
Plant mutants for genes encoding subunits of mitochondrial complex I (CI; NADH:ubiquinone oxidoreductase), the first enzyme respiratory chain, display various phenotypes depending on growth conditions. Here, we examined impact photoperiod, a major environmental factor controlling plant development, two Arabidopsis (Arabidopsis thaliana) CI mutants: new insertion mutant interrupted in both ndufs8.1 and ndufs8.2 NDUFS8 subunit previously characterized ndufs4 mutant. In long day (LD) condition,...
The protoplasts assay constitutes a powerful tool that allows an easy uptake of active agents and precise quantification cell death induction in different populations. Our study showed the basal level our controls is low stable throughout length experiments (Danon et al., 2005; Pineau 2013). In addition, data obtained from protoplast are applicable to intact seedlings, where it possible see differences intensity necrotic lesions 2006) even if those not as easily clearly quantifiable with assay.